Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA
UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound...
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| Veröffentlicht in: | FEBS letters 2006-11, Vol.580 (27), p.6423-6427 |
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| container_title | FEBS letters |
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| creator | Waters, Timothy R. Eryilmaz, Jitka Geddes, Stella Barrett, Tracey E. |
| description | UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide. |
| doi_str_mv | 10.1016/j.febslet.2006.10.051 |
| format | Article |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Crystallography, X-Ray - methods DNA Adducts - chemistry DNA Adducts - metabolism DNA Damage DNA Helicases - chemistry DNA Helicases - metabolism Endodeoxyribonucleases - chemistry Endodeoxyribonucleases - metabolism Escherichia coli - enzymology Escherichia coli Proteins - chemistry Escherichia coli Proteins - metabolism Models, Molecular Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - metabolism Protein crystallography Protein Structure, Secondary Protein Structure, Tertiary T-fluorescein UvrABC |
| title | Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA |
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