Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen

Abstract Traditional methods for enumerating and identifying microbial populations within the rumen can be time consuming and cumbersome. Methods that involve culturing and microscopy can also be inconclusive, particularly when studying anaerobic rumen fungi. A real-time PCR SYBR Green assay, using...

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Veröffentlicht in:	FEMS microbiology ecology 2006-12, Vol.58 (3), p.572-582
Hauptverfasser:	Denman, Stuart E., McSweeney, Christopher S.
Format:	Artikel
Sprache:	eng
Schlagworte:	anaerobic fungi Anaerobiosis Animals Assaying Bacteria Bacteria - genetics Bacteria - isolation & purification Bacteriology Biological and medical sciences Calibration Cattle Cellulolytic bacteria Cellulose - metabolism Colony Count, Microbial Cross-reactivity Ecology Feeding Fibrobacter succinogenes Fundamental and applied biological sciences. Psychology Fungi Fungi - genetics Fungi - isolation & purification Identification methods Microbiology Microorganisms Miscellaneous Mycology Polymerase chain reaction Polymerase Chain Reaction - methods Population studies Populations Real time real‐time PCR Rumen Rumen - microbiology Ruminococcus flavefaciens
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