Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system
A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used as an expression standard. For expression comparisons, various permutations of a soybean polyubiquiti...
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| Veröffentlicht in: | Plant cell reports 2007-09, Vol.26 (9), p.1501-1509 |
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| creator | CHIERA, Joseph M BOUCHARD, Robert A DORSEY, Summer L PARK, Euiho BUENROSTRO-NAVA, Marco T LING, Peter P FINER, John J |
| description | A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used as an expression standard. For expression comparisons, various permutations of a soybean polyubiquitin (Gmubi) promoter, a soybean heat shock protein 90-like (GmHSP90L) promoter and the CaMV35S promoter were placed upstream of a green fluorescent protein (gfp) gene. DNA constructs were introduced via particle bombardment into excised cotyledons of germinating lima bean (Phaseolus lunatus L.) seeds, which were arranged in Petri dishes for automated image capture and image analysis. The automated system allowed monitoring and quantification of gfp gene expression in the same piece of tissue over time. The Gmubi promoter, with its intronic region intact, showed the highest expression that was over five times stronger than the CaMV35S promoter. When an intronic region was removed from the Gmubi promoter, GFP expression was reduced, but was still over two times greater than with the CaMV35S promoter. The full-length soybean GmHSP90L promoter was four times stronger than the CaMV35S promoter. Truncation of the GmHSP90L promoter resulted in stepwise decreases in promoter strength, which appear to correspond to removal of regulatory elements. Automated image capture and analysis allowed the rapid and efficient evaluation of these new promoters. |
| doi_str_mv | 10.1007/s00299-007-0359-y |
| format | Article |
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DNA constructs were introduced via particle bombardment into excised cotyledons of germinating lima bean (Phaseolus lunatus L.) seeds, which were arranged in Petri dishes for automated image capture and image analysis. The automated system allowed monitoring and quantification of gfp gene expression in the same piece of tissue over time. The Gmubi promoter, with its intronic region intact, showed the highest expression that was over five times stronger than the CaMV35S promoter. When an intronic region was removed from the Gmubi promoter, GFP expression was reduced, but was still over two times greater than with the CaMV35S promoter. The full-length soybean GmHSP90L promoter was four times stronger than the CaMV35S promoter. Truncation of the GmHSP90L promoter resulted in stepwise decreases in promoter strength, which appear to correspond to removal of regulatory elements. Automated image capture and analysis allowed the rapid and efficient evaluation of these new promoters.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-007-0359-y</identifier><identifier>PMID: 17503049</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; Cauliflower mosaic virus ; Caulimovirus ; Cotyledons ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Glycine max ; Glycine max - genetics ; Green fluorescent protein ; Heat shock proteins ; HSP90 Heat-Shock Proteins - genetics ; Hsp90 protein ; Image analysis ; Image processing ; Image Processing, Computer-Assisted - methods ; Lima ; Particle bombardment ; Permutations ; Phaseolus lunatus ; Promoter Regions, Genetic - genetics ; Promoters ; Proteins ; Regulatory sequences ; Seeds ; Soybeans ; Ubiquitin - genetics</subject><ispartof>Plant cell reports, 2007-09, Vol.26 (9), p.1501-1509</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-5648e4059d9e8a6b22ccefb8b9d2b643b6cbd0b37c3acedf0b945573f62d25283</citedby><cites>FETCH-LOGICAL-c387t-5648e4059d9e8a6b22ccefb8b9d2b643b6cbd0b37c3acedf0b945573f62d25283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19063204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17503049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHIERA, Joseph M</creatorcontrib><creatorcontrib>BOUCHARD, Robert A</creatorcontrib><creatorcontrib>DORSEY, Summer L</creatorcontrib><creatorcontrib>PARK, Euiho</creatorcontrib><creatorcontrib>BUENROSTRO-NAVA, Marco T</creatorcontrib><creatorcontrib>LING, Peter P</creatorcontrib><creatorcontrib>FINER, John J</creatorcontrib><title>Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used as an expression standard. For expression comparisons, various permutations of a soybean polyubiquitin (Gmubi) promoter, a soybean heat shock protein 90-like (GmHSP90L) promoter and the CaMV35S promoter were placed upstream of a green fluorescent protein (gfp) gene. DNA constructs were introduced via particle bombardment into excised cotyledons of germinating lima bean (Phaseolus lunatus L.) seeds, which were arranged in Petri dishes for automated image capture and image analysis. The automated system allowed monitoring and quantification of gfp gene expression in the same piece of tissue over time. The Gmubi promoter, with its intronic region intact, showed the highest expression that was over five times stronger than the CaMV35S promoter. When an intronic region was removed from the Gmubi promoter, GFP expression was reduced, but was still over two times greater than with the CaMV35S promoter. The full-length soybean GmHSP90L promoter was four times stronger than the CaMV35S promoter. Truncation of the GmHSP90L promoter resulted in stepwise decreases in promoter strength, which appear to correspond to removal of regulatory elements. Automated image capture and analysis allowed the rapid and efficient evaluation of these new promoters.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cauliflower mosaic virus</subject><subject>Caulimovirus</subject><subject>Cotyledons</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Glycine max</subject><subject>Glycine max - genetics</subject><subject>Green fluorescent protein</subject><subject>Heat shock proteins</subject><subject>HSP90 Heat-Shock Proteins - genetics</subject><subject>Hsp90 protein</subject><subject>Image analysis</subject><subject>Image processing</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Lima</subject><subject>Particle bombardment</subject><subject>Permutations</subject><subject>Phaseolus lunatus</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoters</subject><subject>Proteins</subject><subject>Regulatory sequences</subject><subject>Seeds</subject><subject>Soybeans</subject><subject>Ubiquitin - genetics</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU9rFTEUxYMo9ln9AG4kCEpdTM2_mSRLKVoLFTcK7oYkc-e9lMykJhnr-GX8qub5HhTcuLoH7u8cuPcg9JySc0qIfJsJYVo3VTaEt7pZH6ANFZw1jPBvD9GGSEYbKak4QU9yviGkLmX3GJ1Q2RJOhN6g31c5BlN8nHEccbmLeOe3u7Bi44r_ATjH1YKZ8dllWJ2fAU_mJz67Pn-DP0FKddymOMUCKWMzD7jswCfsdiZVPyT_6xC9ZD9vscEz3GGzlDiZAgP2k9kCdjEEcH-xfYKZTVizzzivucD0FD0aTcjw7DhP0dcP779cfGyuP19eXby7bhxXsjRtJxQI0upBgzKdZcw5GK2yemC2E9x2zg7Ecum4cTCMxGrRtpKPHRtYyxQ_Ra8PufWe7wvk0k8-OwjBzBCX3HeKSq5Y-1-QaqUo010FX_4D3sQl1etyr4gUmikpKkQPkEsx5wRjf5vqW9LaU9LvO-4PHfd7ue-4X6vnxTF4sRMM945jqRV4dQRMdiaMyczO53tOk44zIvgfthaxWg</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>CHIERA, Joseph M</creator><creator>BOUCHARD, Robert A</creator><creator>DORSEY, Summer L</creator><creator>PARK, Euiho</creator><creator>BUENROSTRO-NAVA, Marco T</creator><creator>LING, Peter P</creator><creator>FINER, John J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20070901</creationdate><title>Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system</title><author>CHIERA, Joseph M ; BOUCHARD, Robert A ; DORSEY, Summer L ; PARK, Euiho ; BUENROSTRO-NAVA, Marco T ; LING, Peter P ; FINER, John J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-5648e4059d9e8a6b22ccefb8b9d2b643b6cbd0b37c3acedf0b945573f62d25283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cauliflower mosaic virus</topic><topic>Caulimovirus</topic><topic>Cotyledons</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Glycine max</topic><topic>Glycine max - genetics</topic><topic>Green fluorescent protein</topic><topic>Heat shock proteins</topic><topic>HSP90 Heat-Shock Proteins - genetics</topic><topic>Hsp90 protein</topic><topic>Image analysis</topic><topic>Image processing</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Lima</topic><topic>Particle bombardment</topic><topic>Permutations</topic><topic>Phaseolus lunatus</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoters</topic><topic>Proteins</topic><topic>Regulatory sequences</topic><topic>Seeds</topic><topic>Soybeans</topic><topic>Ubiquitin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHIERA, Joseph M</creatorcontrib><creatorcontrib>BOUCHARD, Robert A</creatorcontrib><creatorcontrib>DORSEY, Summer L</creatorcontrib><creatorcontrib>PARK, Euiho</creatorcontrib><creatorcontrib>BUENROSTRO-NAVA, Marco T</creatorcontrib><creatorcontrib>LING, Peter P</creatorcontrib><creatorcontrib>FINER, John J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHIERA, Joseph M</au><au>BOUCHARD, Robert A</au><au>DORSEY, Summer L</au><au>PARK, Euiho</au><au>BUENROSTRO-NAVA, Marco T</au><au>LING, Peter P</au><au>FINER, John J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>26</volume><issue>9</issue><spage>1501</spage><epage>1509</epage><pages>1501-1509</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>A novel automated image collection and analysis system was used to compare two new soybean (Glycine max (L.) Merr.) promoters with the cauliflower mosaic virus 35S (CaMV35S) promoter, which was used as an expression standard. For expression comparisons, various permutations of a soybean polyubiquitin (Gmubi) promoter, a soybean heat shock protein 90-like (GmHSP90L) promoter and the CaMV35S promoter were placed upstream of a green fluorescent protein (gfp) gene. DNA constructs were introduced via particle bombardment into excised cotyledons of germinating lima bean (Phaseolus lunatus L.) seeds, which were arranged in Petri dishes for automated image capture and image analysis. The automated system allowed monitoring and quantification of gfp gene expression in the same piece of tissue over time. The Gmubi promoter, with its intronic region intact, showed the highest expression that was over five times stronger than the CaMV35S promoter. When an intronic region was removed from the Gmubi promoter, GFP expression was reduced, but was still over two times greater than with the CaMV35S promoter. The full-length soybean GmHSP90L promoter was four times stronger than the CaMV35S promoter. Truncation of the GmHSP90L promoter resulted in stepwise decreases in promoter strength, which appear to correspond to removal of regulatory elements. Automated image capture and analysis allowed the rapid and efficient evaluation of these new promoters.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>17503049</pmid><doi>10.1007/s00299-007-0359-y</doi><tpages>9</tpages></addata></record> |
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| subjects | Biological and medical sciences Biotechnology Cauliflower mosaic virus Caulimovirus Cotyledons Fluorescence Fundamental and applied biological sciences. Psychology Gene expression Glycine max Glycine max - genetics Green fluorescent protein Heat shock proteins HSP90 Heat-Shock Proteins - genetics Hsp90 protein Image analysis Image processing Image Processing, Computer-Assisted - methods Lima Particle bombardment Permutations Phaseolus lunatus Promoter Regions, Genetic - genetics Promoters Proteins Regulatory sequences Seeds Soybeans Ubiquitin - genetics |
| title | Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system |
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