A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer

Bioluminescence resonance energy transfer (BRET), which relies on nonradiative energy transfer between luciferase-coupled donors and GFP-coupled acceptors, is emerging as a useful tool for analyzing the quaternary structures of cell-surface molecules. Conventional BRET analyses are generally done at...

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Veröffentlicht in:	Nature methods 2006-12, Vol.3 (12), p.1001-1006
Hauptverfasser:	James, John R, Oliveira, Marta I, Carmo, Alexandre M, Iaboni, Andrea, Davis, Simon J
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Bioinformatics Biological assay Biological Microscopy Biological Techniques Bioluminescence Biomedical and Life Sciences Biomedical Engineering/Biotechnology Cell Membrane - chemistry Cell Membrane - metabolism Cellular biology Dimerization Energy transfer Fluorescence Resonance Energy Transfer - methods Health aspects Life Sciences Luminescent Measurements - methods Luminescent Proteins - metabolism Methods Molecular structure Physiological aspects Protein Binding Protein Interaction Mapping - methods Proteins Proteomics Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - metabolism Research methodology Resonance
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