Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons...
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| Veröffentlicht in: | Proteomics (Weinheim) 2006-09, Vol.6 (S2), p.61-64 |
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| container_title | Proteomics (Weinheim) |
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| creator | Westermeier, Reiner |
| description | In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons:
Low price
Visible with the eye
Desk top scanners can be employed for image acquisition
Better for quantitative analysis than silver staining
Possible modifications for fast or highly sensitive staining
Mass spectrometry compatible |
| doi_str_mv | 10.1002/pmic.200690121 |
| format | Article |
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Low price
Visible with the eye
Desk top scanners can be employed for image acquisition
Better for quantitative analysis than silver staining
Possible modifications for fast or highly sensitive staining
Mass spectrometry compatible</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.200690121</identifier><identifier>PMID: 17031800</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Colloids ; Electrophoresis, Gel, Two-Dimensional - methods ; Proteins - analysis ; Rosaniline Dyes ; Sensitivity and Specificity ; Silver Staining - methods ; Staining and Labeling - methods</subject><ispartof>Proteomics (Weinheim), 2006-09, Vol.6 (S2), p.61-64</ispartof><rights>Copyright © 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4121-7a93b67dac23482058bdf8314fbbde7c1e9b55e72942736329afafef6b9a5363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.200690121$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.200690121$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17031800$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Westermeier, Reiner</creatorcontrib><title>Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons:
Low price
Visible with the eye
Desk top scanners can be employed for image acquisition
Better for quantitative analysis than silver staining
Possible modifications for fast or highly sensitive staining
Mass spectrometry compatible</description><subject>Colloids</subject><subject>Electrophoresis, Gel, Two-Dimensional - methods</subject><subject>Proteins - analysis</subject><subject>Rosaniline Dyes</subject><subject>Sensitivity and Specificity</subject><subject>Silver Staining - methods</subject><subject>Staining and Labeling - methods</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP3DAUha2KqtCh2y4rr1iRqR-JHS_LCAYkoDxGQmJj3STXlWkSD3FCO_--QRkN3aG7uA995-jqEPKVszlnTHxfN76cC8aUYVzwD-SAK54lJld8bzdncp98jvGJMa5zoz-Rfa6Z5DljB6S4xzb63r_gMb0doO19D9MGbUXPIPb0KlTe-XI8hzZSFzq6CKGBGD3Sk3pAet-Db337iwZHb0K9gbLb1ND4CukS63hIPjqoI37Z9hlZnZ2uFufJ5c_lxeLHZVKm4-uJBiMLpSsohUxzwbK8qFwueeqKokJdcjRFlqEWJhVaKikMOHDoVGEgG_cZOZps1114HjD2tvGxxLqGFsMQrcq5FpnJ3wW5MVqmY83IfALLLsTYobPrzjfQbSxn9jV9-5q-3aU_Cr5tnYeiweoN38Y9AmYC_vgaN-_Y2Zuri8X_5smk9bHHvzstdL-t0lJn9uF6aR9PVuqR353ba_kPzGWhGA</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>Westermeier, Reiner</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels</title><author>Westermeier, Reiner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4121-7a93b67dac23482058bdf8314fbbde7c1e9b55e72942736329afafef6b9a5363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Colloids</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Proteins - analysis</topic><topic>Rosaniline Dyes</topic><topic>Sensitivity and Specificity</topic><topic>Silver Staining - methods</topic><topic>Staining and Labeling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Westermeier, Reiner</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Westermeier, Reiner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2006-09</date><risdate>2006</risdate><volume>6</volume><issue>S2</issue><spage>61</spage><epage>64</epage><pages>61-64</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons:
Low price
Visible with the eye
Desk top scanners can be employed for image acquisition
Better for quantitative analysis than silver staining
Possible modifications for fast or highly sensitive staining
Mass spectrometry compatible</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>17031800</pmid><doi>10.1002/pmic.200690121</doi><tpages>4</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Colloids Electrophoresis, Gel, Two-Dimensional - methods Proteins - analysis Rosaniline Dyes Sensitivity and Specificity Silver Staining - methods Staining and Labeling - methods |
| title | Sensitive, Quantitative, and Fast Modifications for Coomassie Blue Staining of Polyacrylamide Gels |
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