Transactivation of protein expression by rice [Oryza sativa] HSP101 in planta and using Hsp101 as a selection marker for transformation
Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene in...
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| Veröffentlicht in: | Plant and cell physiology 2007-08, Vol.48 (8), p.1098-1107 |
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| creator | Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan)) Huang, P.S Lin, H.R Lu,C.H |
| description | Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines. |
| doi_str_mv | 10.1093/pcp/pcm080 |
| format | Article |
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In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcm080</identifier><identifier>PMID: 17597080</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>Agrobacterium ; Blotting, Southern ; Blotting, Western ; Gene Expression Regulation, Plant ; GENETIC ENGINEERING ; GENETIC MARKERS ; Genetic Vectors - genetics ; GENIE GENETIQUE ; HEAT TOLERANCE ; Hot Temperature ; HSP101 ; IMMUNOLOGICAL TECHNIQUES ; INGENIERIA GENETICA ; Luciferases - genetics ; Luciferases - metabolism ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; Nicotiana - genetics ; Nicotiana - metabolism ; NICOTIANA TABACUM ; NUCLEOTIDE SEQUENCE ; Oryza - genetics ; Oryza - metabolism ; ORYZA SATIVA ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; SECUENCIA NUCLEOTIDICA ; SELECCION ; SELECTION ; Selection marker ; SEQUENCE NUCLEOTIDIQUE ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS ; Temperature ; Thermotolerance ; TOLERANCE A LA CHALEUR ; TOLERANCIA AL CALOR ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transformation, Genetic ; Transgenic tobacco ; Translational activator</subject><ispartof>Plant and cell physiology, 2007-08, Vol.48 (8), p.1098-1107</ispartof><rights>The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org 2007</rights><rights>The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-b1d3fd91b4e32988ffeb77af153d79f9199ebb7636b609fa3d84da364134f8903</citedby><cites>FETCH-LOGICAL-c518t-b1d3fd91b4e32988ffeb77af153d79f9199ebb7636b609fa3d84da364134f8903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17597080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan))</creatorcontrib><creatorcontrib>Huang, P.S</creatorcontrib><creatorcontrib>Lin, H.R</creatorcontrib><creatorcontrib>Lu,C.H</creatorcontrib><title>Transactivation of protein expression by rice [Oryza sativa] HSP101 in planta and using Hsp101 as a selection marker for transformation</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.</description><subject>Agrobacterium</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Gene Expression Regulation, Plant</subject><subject>GENETIC ENGINEERING</subject><subject>GENETIC MARKERS</subject><subject>Genetic Vectors - genetics</subject><subject>GENIE GENETIQUE</subject><subject>HEAT TOLERANCE</subject><subject>Hot Temperature</subject><subject>HSP101</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>INGENIERIA GENETICA</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>NICOTIANA TABACUM</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>ORYZA SATIVA</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SELECCION</subject><subject>SELECTION</subject><subject>Selection marker</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><subject>Temperature</subject><subject>Thermotolerance</subject><subject>TOLERANCE A LA CHALEUR</subject><subject>TOLERANCIA AL CALOR</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transformation, Genetic</subject><subject>Transgenic tobacco</subject><subject>Translational activator</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0k1v1DAQBmALgehSuHAHLCQ4IAVm1kkcH9EWWNqKVmwrVSBkOYldpWw-aieoyx_gbzPZrEDqAQ6RrfjRjCdvGHuM8BpBiTdd0dFTQwZ32AxjiZGCRNxlMwAxj0BmuMcehHAFQHsB99keykRJ8jP268ybJpiir36Yvmob3jre-ba3VcPtTedtCOPbfMN9VVj-9cRvfhoezOi_8eXqFAE52W5tmt5w05R8CFVzyZehG49M4MTt2hbb6rXx363nrvW8HxvTpt72fcjuObMO9tFu3Wfn79-dLZbR8cmHj4u3x1GRYNZHOZbClQrz2Iq5yjLnbC6lcZiIUiqnUCmb5zIVaZ6CckaUWVwakcYoYpcpEPvs5VSXhrwebOh1XYXCrun6th2CTjOUkIL8L5yDVKnYwue34FU7-IaGIINJQl0VoVcTKnwbgrdOd76ij7HRCHoMUVOIegqR8NNdxSGvbfmX7lIj8GIC7dD9u1A0uSr09uaPpAx0KoVM9PLii_60OlxcHHw-0kfkn03emVabS18Ffb6iIQT9OEoBpiSe3BaHp3OgZoAynovfGCnGnQ</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan))</creator><creator>Huang, P.S</creator><creator>Lin, H.R</creator><creator>Lu,C.H</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>Transactivation of protein expression by rice [Oryza sativa] HSP101 in planta and using Hsp101 as a selection marker for transformation</title><author>Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan)) ; Huang, P.S ; Lin, H.R ; Lu,C.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-b1d3fd91b4e32988ffeb77af153d79f9199ebb7636b609fa3d84da364134f8903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agrobacterium</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Gene Expression Regulation, Plant</topic><topic>GENETIC ENGINEERING</topic><topic>GENETIC MARKERS</topic><topic>Genetic Vectors - genetics</topic><topic>GENIE GENETIQUE</topic><topic>HEAT TOLERANCE</topic><topic>Hot Temperature</topic><topic>HSP101</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>INGENIERIA GENETICA</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>NICOTIANA TABACUM</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>ORYZA SATIVA</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SELECCION</topic><topic>SELECTION</topic><topic>Selection marker</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><topic>Temperature</topic><topic>Thermotolerance</topic><topic>TOLERANCE A LA CHALEUR</topic><topic>TOLERANCIA AL CALOR</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transformation, Genetic</topic><topic>Transgenic tobacco</topic><topic>Translational activator</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan))</creatorcontrib><creatorcontrib>Huang, P.S</creatorcontrib><creatorcontrib>Lin, H.R</creatorcontrib><creatorcontrib>Lu,C.H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, C.C.(National Cheng Kung Univ., Tainan (Taiwan))</au><au>Huang, P.S</au><au>Lin, H.R</au><au>Lu,C.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transactivation of protein expression by rice [Oryza sativa] HSP101 in planta and using Hsp101 as a selection marker for transformation</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>48</volume><issue>8</issue><spage>1098</spage><epage>1107</epage><pages>1098-1107</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Plant HSP101 has dual activities, first, in conferring thermotolerance, and secondly, in serving as a translational activator. In this study, we introduced Oryza sativa Hsp101 (osHsp101) cDNA into tobacco by Agrobacterium-mediated transformation. Stable integration and expression of the transgene into the tobacco genome was demonstrated by Southern and Western blot analysis. Overexpression of osHSP101 had no noticeable effect on growth or development of the transgenic plants. Homozygous T2 transgenic plants with overexpressed osHSP101 survived heat treatment better than untransformed control plants. In addition, taking advantage of conferring basal thermotolerance by plant HSP101, we were able to demonstrate the feasibility of using osHsp101 as a selection marker and select the transformants under high temperature in tobacco leaf disc transformation mediated by Agrobacterium. Furthermore, transgenic tobacco plants with overexpressed osHSP101 were able to enhance luciferase expression up to 2.9-fold more than untransformed plants in the progeny of reciprocally crossed with omega-luciferase reporter lines.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>17597080</pmid><doi>10.1093/pcp/pcm080</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 2007-08, Vol.48 (8), p.1098-1107 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Agrobacterium Blotting, Southern Blotting, Western Gene Expression Regulation, Plant GENETIC ENGINEERING GENETIC MARKERS Genetic Vectors - genetics GENIE GENETIQUE HEAT TOLERANCE Hot Temperature HSP101 IMMUNOLOGICAL TECHNIQUES INGENIERIA GENETICA Luciferases - genetics Luciferases - metabolism MARCADORES GENETICOS MARQUEUR GENETIQUE Nicotiana - genetics Nicotiana - metabolism NICOTIANA TABACUM NUCLEOTIDE SEQUENCE Oryza - genetics Oryza - metabolism ORYZA SATIVA Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism SECUENCIA NUCLEOTIDICA SELECCION SELECTION Selection marker SEQUENCE NUCLEOTIDIQUE TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS Temperature Thermotolerance TOLERANCE A LA CHALEUR TOLERANCIA AL CALOR Transcription Factors - genetics Transcription Factors - metabolism Transformation, Genetic Transgenic tobacco Translational activator |
| title | Transactivation of protein expression by rice [Oryza sativa] HSP101 in planta and using Hsp101 as a selection marker for transformation |
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