Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation

We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the constr...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2007-07, Vol.104 (1), p.34-41
Hauptverfasser:	Nakagawa, Tsuyoshi, Kurose, Takayuki, Hino, Takeshi, Tanaka, Katsunori, Kawamukai, Makoto, Niwa, Yasuo, Toyooka, Kiminori, Matsuoka, Ken, Jinbo, Tetsuro, Kimura, Tetsuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Artificial Gene Fusion Base Sequence binary vector Biological and medical sciences Biotechnology Cauliflower mosaic virus Caulimovirus - genetics Cell Line CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular - methods Fundamental and applied biological sciences. Psychology gateway cloning GENE Gene Fusion GENES Genes, Reporter GENETIC ENGINEERING GENETIC TRANSFORMATION GENETIC VECTORS Genetic Vectors - genetics GENIE GENETIQUE Glucuronidase - analysis Glucuronidase - genetics Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics INGENIERIA GENETICA MOLECULAR CLONING Molecular Sequence Data Phosphotransferases (Alcohol Group Acceptor) - analysis Phosphotransferases (Alcohol Group Acceptor) - genetics plant PLANTE PLANTS Plants - chemistry Plants - genetics Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics tag TRANSFORMACION GENETICA transformation TRANSFORMATION GENETIQUE Transformation, Genetic VECTEUR GENETIQUE VECTORES GENETICOS
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