Research of cardiomyocyte precursors in adult rat heart
Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populat...
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| Veröffentlicht in: | Tissue & cell 2006-12, Vol.38 (6), p.345-351 |
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| creator | Bellafiore, M. Sivverini, G. Cappello, F. David, S. Palma, A. Farina, F. Zummo, G. |
| description | Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability. |
| doi_str_mv | 10.1016/j.tice.2006.08.003 |
| format | Article |
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However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability.</description><identifier>ISSN: 0040-8166</identifier><identifier>EISSN: 1532-3072</identifier><identifier>DOI: 10.1016/j.tice.2006.08.003</identifier><identifier>PMID: 17101162</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Animals ; Antigens - metabolism ; Antigens, CD34 - metabolism ; Cardiac progenitor markers ; Cardiac resident stem cells ; Cell Differentiation ; Cell Lineage ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; GATA4 Transcription Factor - metabolism ; Intermediate Filament Proteins - metabolism ; Male ; Myocardium - cytology ; Myocardium - metabolism ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Nerve Tissue Proteins - metabolism ; Nestin ; Proto-Oncogene Proteins c-kit - metabolism ; Rat myocardium ; Rats ; Rats, Wistar ; Stem Cells - cytology</subject><ispartof>Tissue & cell, 2006-12, Vol.38 (6), p.345-351</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-3b6a1454603fa3e42639fa382f55b17d4452fca7562c67dab3731651920398353</citedby><cites>FETCH-LOGICAL-c398t-3b6a1454603fa3e42639fa382f55b17d4452fca7562c67dab3731651920398353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tice.2006.08.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17101162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bellafiore, M.</creatorcontrib><creatorcontrib>Sivverini, G.</creatorcontrib><creatorcontrib>Cappello, F.</creatorcontrib><creatorcontrib>David, S.</creatorcontrib><creatorcontrib>Palma, A.</creatorcontrib><creatorcontrib>Farina, F.</creatorcontrib><creatorcontrib>Zummo, G.</creatorcontrib><title>Research of cardiomyocyte precursors in adult rat heart</title><title>Tissue & cell</title><addtitle>Tissue Cell</addtitle><description>Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability.</description><subject>Animals</subject><subject>Antigens - metabolism</subject><subject>Antigens, CD34 - metabolism</subject><subject>Cardiac progenitor markers</subject><subject>Cardiac resident stem cells</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>GATA4 Transcription Factor - metabolism</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Male</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Nestin</subject><subject>Proto-Oncogene Proteins c-kit - metabolism</subject><subject>Rat myocardium</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stem Cells - cytology</subject><issn>0040-8166</issn><issn>1532-3072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEURYMotlb_gAuZlbsZX5JJMgU3In5BQRBdh0zmDU2ZNjXJCP33prTgztV7i3Mv3EPINYWKApV3qyo5ixUDkBU0FQA_IVMqOCs5KHZKpgA1lA2VckIuYlwBgKqpOicTqnIBlWxK1AdGNMEuC98X1oTO-fXO213CYhvQjiH6EAu3KUw3DqkIJhXLzKdLctabIeLV8c7I1_PT5-NruXh_eXt8WJSWz5tU8lYaWotaAu8Nx5pJPs9Pw3ohWqq6uhast0YJyaxUnWm54lQKOmeQ81zwGbk99G6D_x4xJr120eIwmA36MWqZ5zVcygyyA2iDjzFgr7fBrU3YaQp6r0uv9F6X3uvS0OisK4duju1ju8buL3L0k4H7A4B544_DoKN1uLHYuWwn6c67__p_AQWMebM</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Bellafiore, M.</creator><creator>Sivverini, G.</creator><creator>Cappello, F.</creator><creator>David, S.</creator><creator>Palma, A.</creator><creator>Farina, F.</creator><creator>Zummo, G.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Research of cardiomyocyte precursors in adult rat heart</title><author>Bellafiore, M. ; Sivverini, G. ; Cappello, F. ; David, S. ; Palma, A. ; Farina, F. ; Zummo, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-3b6a1454603fa3e42639fa382f55b17d4452fca7562c67dab3731651920398353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens - metabolism</topic><topic>Antigens, CD34 - metabolism</topic><topic>Cardiac progenitor markers</topic><topic>Cardiac resident stem cells</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - metabolism</topic><topic>GATA4 Transcription Factor - metabolism</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Male</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Nestin</topic><topic>Proto-Oncogene Proteins c-kit - metabolism</topic><topic>Rat myocardium</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stem Cells - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bellafiore, M.</creatorcontrib><creatorcontrib>Sivverini, G.</creatorcontrib><creatorcontrib>Cappello, F.</creatorcontrib><creatorcontrib>David, S.</creatorcontrib><creatorcontrib>Palma, A.</creatorcontrib><creatorcontrib>Farina, F.</creatorcontrib><creatorcontrib>Zummo, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue & cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bellafiore, M.</au><au>Sivverini, G.</au><au>Cappello, F.</au><au>David, S.</au><au>Palma, A.</au><au>Farina, F.</au><au>Zummo, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Research of cardiomyocyte precursors in adult rat heart</atitle><jtitle>Tissue & cell</jtitle><addtitle>Tissue Cell</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>38</volume><issue>6</issue><spage>345</spage><epage>351</epage><pages>345-351</pages><issn>0040-8166</issn><eissn>1532-3072</eissn><abstract>Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>17101162</pmid><doi>10.1016/j.tice.2006.08.003</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens - metabolism Antigens, CD34 - metabolism Cardiac progenitor markers Cardiac resident stem cells Cell Differentiation Cell Lineage Endothelial Cells - cytology Endothelial Cells - metabolism GATA4 Transcription Factor - metabolism Intermediate Filament Proteins - metabolism Male Myocardium - cytology Myocardium - metabolism Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Nerve Tissue Proteins - metabolism Nestin Proto-Oncogene Proteins c-kit - metabolism Rat myocardium Rats Rats, Wistar Stem Cells - cytology |
| title | Research of cardiomyocyte precursors in adult rat heart |
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