Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules

We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vec...

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Veröffentlicht in:	Plant cell reports 2006-12, Vol.25 (12), p.1325-1335
Hauptverfasser:	HOLME, Inger Baeksted, BRINCH-PEDERSEN, Henrik, LANGE, Mette, HOLM, Preben Bach
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Agrobacterium tumefaciens - pathogenicity Agrobacterium tumefaciens - physiology Bacteria Barley Biological and medical sciences Biotechnology Blotting, Southern Cultivars DNA, Bacterial - metabolism DNA, Plant - metabolism Fundamental and applied biological sciences. Psychology Gene Dosage Genetic engineering Genetic technics Genetic Vectors Green Fluorescent Proteins - metabolism Hordeum - cytology Hordeum - genetics Hordeum - microbiology Hordeum vulgare Inheritance Patterns Methods. Procedures. Technologies Molecular Weight Pollination Regeneration Seeds - cytology Seeds - genetics Seeds - microbiology Transformation, Genetic Transgenes Transgenic animals and transgenic plants Transgenic plants
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