Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules
We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vec...
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| Veröffentlicht in: | Plant cell reports 2006-12, Vol.25 (12), p.1325-1335 |
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| creator | HOLME, Inger Baeksted BRINCH-PEDERSEN, Henrik LANGE, Mette HOLM, Preben Bach |
| description | We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants. |
| doi_str_mv | 10.1007/s00299-006-0188-4 |
| format | Article |
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Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-006-0188-4</identifier><identifier>PMID: 16832622</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agrobacterium tumefaciens ; Agrobacterium tumefaciens - pathogenicity ; Agrobacterium tumefaciens - physiology ; Bacteria ; Barley ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; Cultivars ; DNA, Bacterial - metabolism ; DNA, Plant - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Dosage ; Genetic engineering ; Genetic technics ; Genetic Vectors ; Green Fluorescent Proteins - metabolism ; Hordeum - cytology ; Hordeum - genetics ; Hordeum - microbiology ; Hordeum vulgare ; Inheritance Patterns ; Methods. Procedures. Technologies ; Molecular Weight ; Pollination ; Regeneration ; Seeds - cytology ; Seeds - genetics ; Seeds - microbiology ; Transformation, Genetic ; Transgenes ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>Plant cell reports, 2006-12, Vol.25 (12), p.1325-1335</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-cb22c8b1805fd1204fcfe00ee27333264c2f4b6d03b1a4c1b6d132774274b4373</citedby><cites>FETCH-LOGICAL-c430t-cb22c8b1805fd1204fcfe00ee27333264c2f4b6d03b1a4c1b6d132774274b4373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18319754$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16832622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HOLME, Inger Baeksted</creatorcontrib><creatorcontrib>BRINCH-PEDERSEN, Henrik</creatorcontrib><creatorcontrib>LANGE, Mette</creatorcontrib><creatorcontrib>HOLM, Preben Bach</creatorcontrib><title>Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants.</description><subject>Agrobacterium tumefaciens</subject><subject>Agrobacterium tumefaciens - pathogenicity</subject><subject>Agrobacterium tumefaciens - physiology</subject><subject>Bacteria</subject><subject>Barley</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Cultivars</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Plant - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Dosage</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hordeum - cytology</subject><subject>Hordeum - genetics</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Inheritance Patterns</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Weight</subject><subject>Pollination</subject><subject>Regeneration</subject><subject>Seeds - cytology</subject><subject>Seeds - genetics</subject><subject>Seeds - microbiology</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUFrFTEQx4Mo9vn0A3iRICh62DqTZDe7x1JsKzzwUsFbSLJJSdndPJNN4X37prwnBS-eMjC_-ZOZHyHvEc4RQH7LAGwYGoCuAez7RrwgGxScNQz475dkA5JhIyWKM_Im53uA2pTda3KGXc9Zx9iG7G-TXrKPadZriAuNnhqdJnegX25iGl2Z6UOZ7nRydHf-lZoDvbhL0Wi7uhRqcy2z89oGt2QaFu_s35Sw0IewpkhtmdaS3EhjDXL5LXnl9ZTdu9O7Jb-uvt9e3jS7n9c_Li92jRUc1sYaxmxvsIfWj8hAeOsdgHNMcl7_LizzwnQjcINaWKwlcialYFKYuiXfks_H3H2Kf4rLq5pDtm6a9OJiyarr6xFYx_8L4tDKoasX3ZKP_4D3saSlLqF6kC0iiLZCeIRsijkn59U-hVmng0JQT9LUUZqq0tSTNCXqzIdTcDGzG58nTpYq8OkE6Gz15KsyG_Iz13McZCv4I4Kxntw</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>HOLME, Inger Baeksted</creator><creator>BRINCH-PEDERSEN, Henrik</creator><creator>LANGE, Mette</creator><creator>HOLM, Preben Bach</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules</title><author>HOLME, Inger Baeksted ; BRINCH-PEDERSEN, Henrik ; LANGE, Mette ; HOLM, Preben Bach</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-cb22c8b1805fd1204fcfe00ee27333264c2f4b6d03b1a4c1b6d132774274b4373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Agrobacterium tumefaciens</topic><topic>Agrobacterium tumefaciens - pathogenicity</topic><topic>Agrobacterium tumefaciens - physiology</topic><topic>Bacteria</topic><topic>Barley</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Cultivars</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Plant - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Dosage</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Hordeum - cytology</topic><topic>Hordeum - genetics</topic><topic>Hordeum - microbiology</topic><topic>Hordeum vulgare</topic><topic>Inheritance Patterns</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Weight</topic><topic>Pollination</topic><topic>Regeneration</topic><topic>Seeds - cytology</topic><topic>Seeds - genetics</topic><topic>Seeds - microbiology</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOLME, Inger Baeksted</creatorcontrib><creatorcontrib>BRINCH-PEDERSEN, Henrik</creatorcontrib><creatorcontrib>LANGE, Mette</creatorcontrib><creatorcontrib>HOLM, Preben Bach</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOLME, Inger Baeksted</au><au>BRINCH-PEDERSEN, Henrik</au><au>LANGE, Mette</au><au>HOLM, Preben Bach</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>25</volume><issue>12</issue><spage>1325</spage><epage>1335</epage><pages>1325-1335</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>16832622</pmid><doi>10.1007/s00299-006-0188-4</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agrobacterium tumefaciens Agrobacterium tumefaciens - pathogenicity Agrobacterium tumefaciens - physiology Bacteria Barley Biological and medical sciences Biotechnology Blotting, Southern Cultivars DNA, Bacterial - metabolism DNA, Plant - metabolism Fundamental and applied biological sciences. Psychology Gene Dosage Genetic engineering Genetic technics Genetic Vectors Green Fluorescent Proteins - metabolism Hordeum - cytology Hordeum - genetics Hordeum - microbiology Hordeum vulgare Inheritance Patterns Methods. Procedures. Technologies Molecular Weight Pollination Regeneration Seeds - cytology Seeds - genetics Seeds - microbiology Transformation, Genetic Transgenes Transgenic animals and transgenic plants Transgenic plants |
| title | Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules |
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