Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd
In a preliminary study, we commonly identified Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for G. duodenalis infections for students and s...
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| Veröffentlicht in: | Veterinary parasitology 2006-12, Vol.142 (3), p.231-237 |
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| creator | Uehlinger, Fabienne D. Barkema, Herman W. Dixon, Brent R. Coklin, Tatjana O’Handley, Ryan M. |
| description | In a preliminary study, we commonly identified
Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for
G. duodenalis infections for students and staff at the veterinary college. Fecal samples were collected bi-weekly from this herd of adult cattle (
n
=
30) over an 8-month period to determine the prevalence of
G. duodenalis and
Cryptosporidium spp. within the herd. Nested PCR followed by DNA sequencing was then performed on a subset of positive samples in order to better understand the zoonotic potential of these infections. Every cow was sampled between 11 and 18 times, depending on the date the animal joined the teaching herd. In total, 507 fecal samples were collected from 30 different cows and examined for cysts and oocysts using epifluorescence microscopy.
G. duodenalis prevalence during the course of the study ranged from 37% (11/30) to 64% (18/28), with a mean of 49%. Cumulative
G. duodenalis prevalence was 73% (22/30). Zoonotic
G. duodenalis assemblage A genotype was identified in 43% (6/14) of the
G. duodenalis-positive samples on which PCR and genetic sequencing were successfully performed.
G. duodenalis assemblage E was identified in 57% (8/14) of these samples.
Cryptosporidium spp. oocysts were not detected in the feces of any cows during the study period. The presence of the zoonotic
G. duodenalis assemblage A in 43% of the sequenced samples indicates that there is a potential risk of infection for students and staff at this research and teaching facility, although the roles of cows as sources of giardiasis in humans remain uncertain. Furthermore, due to the large amount of feces they produce, adult cattle may serve as important sources for
G. duodenalis infections in young cattle, or other animals in the facility, despite relatively low numbers of cysts excreted per gram of feces. In contrast, the results of this study indicate that this herd posed a negligible risk of transmitting
Cryptosporidium parvum infections to humans. |
| doi_str_mv | 10.1016/j.vetpar.2006.07.007 |
| format | Article |
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Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for
G. duodenalis infections for students and staff at the veterinary college. Fecal samples were collected bi-weekly from this herd of adult cattle (
n
=
30) over an 8-month period to determine the prevalence of
G. duodenalis and
Cryptosporidium spp. within the herd. Nested PCR followed by DNA sequencing was then performed on a subset of positive samples in order to better understand the zoonotic potential of these infections. Every cow was sampled between 11 and 18 times, depending on the date the animal joined the teaching herd. In total, 507 fecal samples were collected from 30 different cows and examined for cysts and oocysts using epifluorescence microscopy.
G. duodenalis prevalence during the course of the study ranged from 37% (11/30) to 64% (18/28), with a mean of 49%. Cumulative
G. duodenalis prevalence was 73% (22/30). Zoonotic
G. duodenalis assemblage A genotype was identified in 43% (6/14) of the
G. duodenalis-positive samples on which PCR and genetic sequencing were successfully performed.
G. duodenalis assemblage E was identified in 57% (8/14) of these samples.
Cryptosporidium spp. oocysts were not detected in the feces of any cows during the study period. The presence of the zoonotic
G. duodenalis assemblage A in 43% of the sequenced samples indicates that there is a potential risk of infection for students and staff at this research and teaching facility, although the roles of cows as sources of giardiasis in humans remain uncertain. Furthermore, due to the large amount of feces they produce, adult cattle may serve as important sources for
G. duodenalis infections in young cattle, or other animals in the facility, despite relatively low numbers of cysts excreted per gram of feces. In contrast, the results of this study indicate that this herd posed a negligible risk of transmitting
Cryptosporidium parvum infections to humans.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2006.07.007</identifier><identifier>PMID: 16905259</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - parasitology ; cryptosporidiosis ; Cryptosporidiosis - epidemiology ; Cryptosporidium ; Cryptosporidium - isolation & purification ; Cryptosporidium parvum ; dairy cows ; dairy herds ; disease prevalence ; feces ; Feces - parasitology ; Female ; genotype ; Genotyping ; Giardia - isolation & purification ; Giardia duodenalis ; Giardia lamblia ; giardiasis ; Giardiasis - epidemiology ; molecular systematics ; pathogen identification ; Polymerase Chain Reaction - veterinary ; Prevalence ; risk assessment ; Schools, Veterinary ; Time Factors ; veterinary education ; zoonoses ; Zoonoses - epidemiology ; Zoonoses - parasitology ; Zoonotic risk</subject><ispartof>Veterinary parasitology, 2006-12, Vol.142 (3), p.231-237</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-a5db13fd1d39610035c107e156f8d027db2641130a06fc102001af288fb2c7423</citedby><cites>FETCH-LOGICAL-c415t-a5db13fd1d39610035c107e156f8d027db2641130a06fc102001af288fb2c7423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2006.07.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16905259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uehlinger, Fabienne D.</creatorcontrib><creatorcontrib>Barkema, Herman W.</creatorcontrib><creatorcontrib>Dixon, Brent R.</creatorcontrib><creatorcontrib>Coklin, Tatjana</creatorcontrib><creatorcontrib>O’Handley, Ryan M.</creatorcontrib><title>Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>In a preliminary study, we commonly identified
Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for
G. duodenalis infections for students and staff at the veterinary college. Fecal samples were collected bi-weekly from this herd of adult cattle (
n
=
30) over an 8-month period to determine the prevalence of
G. duodenalis and
Cryptosporidium spp. within the herd. Nested PCR followed by DNA sequencing was then performed on a subset of positive samples in order to better understand the zoonotic potential of these infections. Every cow was sampled between 11 and 18 times, depending on the date the animal joined the teaching herd. In total, 507 fecal samples were collected from 30 different cows and examined for cysts and oocysts using epifluorescence microscopy.
G. duodenalis prevalence during the course of the study ranged from 37% (11/30) to 64% (18/28), with a mean of 49%. Cumulative
G. duodenalis prevalence was 73% (22/30). Zoonotic
G. duodenalis assemblage A genotype was identified in 43% (6/14) of the
G. duodenalis-positive samples on which PCR and genetic sequencing were successfully performed.
G. duodenalis assemblage E was identified in 57% (8/14) of these samples.
Cryptosporidium spp. oocysts were not detected in the feces of any cows during the study period. The presence of the zoonotic
G. duodenalis assemblage A in 43% of the sequenced samples indicates that there is a potential risk of infection for students and staff at this research and teaching facility, although the roles of cows as sources of giardiasis in humans remain uncertain. Furthermore, due to the large amount of feces they produce, adult cattle may serve as important sources for
G. duodenalis infections in young cattle, or other animals in the facility, despite relatively low numbers of cysts excreted per gram of feces. In contrast, the results of this study indicate that this herd posed a negligible risk of transmitting
Cryptosporidium parvum infections to humans.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - parasitology</subject><subject>cryptosporidiosis</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium parvum</subject><subject>dairy cows</subject><subject>dairy herds</subject><subject>disease prevalence</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>genotype</subject><subject>Genotyping</subject><subject>Giardia - isolation & purification</subject><subject>Giardia duodenalis</subject><subject>Giardia lamblia</subject><subject>giardiasis</subject><subject>Giardiasis - epidemiology</subject><subject>molecular systematics</subject><subject>pathogen identification</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Prevalence</subject><subject>risk assessment</subject><subject>Schools, Veterinary</subject><subject>Time Factors</subject><subject>veterinary education</subject><subject>zoonoses</subject><subject>Zoonoses - epidemiology</subject><subject>Zoonoses - parasitology</subject><subject>Zoonotic risk</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhHyDwiVvCTBLbyQUJrUpBqsQBKnGzHHuy9SobBztZqf8eV1mJG5zmMM98vA9jbxFKBJQfj-WZltnEsgKQJagSQD1jO2xVXVRCwHO2gxqaogFUV-xVSkcAaECql-wKZQeiEt2O_br1JjpvuFuDo8mMPnEzOb6Pj_MS0hyid3498TTPJfcTNzxfpegnEx-5DeNIB-J9OPuJ-ELGPvjpwB8outfsxWDGRG8u9Zrdf7n5uf9a3H2__bb_fFfYBsVSGOF6rAeHru4kAtTCIihCIYfWQaVcX8kGsQYDcsitHBbNULXt0FdWNVV9zT5se-cYfq-UFn3yydI4monCmrRsUUqQ3X9B7BrVCaUy2GygjSGlSIOeoz_lvBpBP6nXR72p10_qNSid1eexd5f9a38i93fo4joD7zdgMEGbQ_RJ3_-oIGdDxBZQZOLTRlAWdvYUdbKeJkvOR7KLdsH_-4c_Hjefiw</recordid><startdate>20061220</startdate><enddate>20061220</enddate><creator>Uehlinger, Fabienne D.</creator><creator>Barkema, Herman W.</creator><creator>Dixon, Brent R.</creator><creator>Coklin, Tatjana</creator><creator>O’Handley, Ryan M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20061220</creationdate><title>Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd</title><author>Uehlinger, Fabienne D. ; Barkema, Herman W. ; Dixon, Brent R. ; Coklin, Tatjana ; O’Handley, Ryan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-a5db13fd1d39610035c107e156f8d027db2641130a06fc102001af288fb2c7423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - parasitology</topic><topic>cryptosporidiosis</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium parvum</topic><topic>dairy cows</topic><topic>dairy herds</topic><topic>disease prevalence</topic><topic>feces</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>genotype</topic><topic>Genotyping</topic><topic>Giardia - isolation & purification</topic><topic>Giardia duodenalis</topic><topic>Giardia lamblia</topic><topic>giardiasis</topic><topic>Giardiasis - epidemiology</topic><topic>molecular systematics</topic><topic>pathogen identification</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Prevalence</topic><topic>risk assessment</topic><topic>Schools, Veterinary</topic><topic>Time Factors</topic><topic>veterinary education</topic><topic>zoonoses</topic><topic>Zoonoses - epidemiology</topic><topic>Zoonoses - parasitology</topic><topic>Zoonotic risk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uehlinger, Fabienne D.</creatorcontrib><creatorcontrib>Barkema, Herman W.</creatorcontrib><creatorcontrib>Dixon, Brent R.</creatorcontrib><creatorcontrib>Coklin, Tatjana</creatorcontrib><creatorcontrib>O’Handley, Ryan M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uehlinger, Fabienne D.</au><au>Barkema, Herman W.</au><au>Dixon, Brent R.</au><au>Coklin, Tatjana</au><au>O’Handley, Ryan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2006-12-20</date><risdate>2006</risdate><volume>142</volume><issue>3</issue><spage>231</spage><epage>237</epage><pages>231-237</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>In a preliminary study, we commonly identified
Giardia duodenalis in adult dairy cattle from a veterinary college teaching herd. Therefore, the present study was carried out in order to better understand the potential of adult cattle to act as a source for
G. duodenalis infections for students and staff at the veterinary college. Fecal samples were collected bi-weekly from this herd of adult cattle (
n
=
30) over an 8-month period to determine the prevalence of
G. duodenalis and
Cryptosporidium spp. within the herd. Nested PCR followed by DNA sequencing was then performed on a subset of positive samples in order to better understand the zoonotic potential of these infections. Every cow was sampled between 11 and 18 times, depending on the date the animal joined the teaching herd. In total, 507 fecal samples were collected from 30 different cows and examined for cysts and oocysts using epifluorescence microscopy.
G. duodenalis prevalence during the course of the study ranged from 37% (11/30) to 64% (18/28), with a mean of 49%. Cumulative
G. duodenalis prevalence was 73% (22/30). Zoonotic
G. duodenalis assemblage A genotype was identified in 43% (6/14) of the
G. duodenalis-positive samples on which PCR and genetic sequencing were successfully performed.
G. duodenalis assemblage E was identified in 57% (8/14) of these samples.
Cryptosporidium spp. oocysts were not detected in the feces of any cows during the study period. The presence of the zoonotic
G. duodenalis assemblage A in 43% of the sequenced samples indicates that there is a potential risk of infection for students and staff at this research and teaching facility, although the roles of cows as sources of giardiasis in humans remain uncertain. Furthermore, due to the large amount of feces they produce, adult cattle may serve as important sources for
G. duodenalis infections in young cattle, or other animals in the facility, despite relatively low numbers of cysts excreted per gram of feces. In contrast, the results of this study indicate that this herd posed a negligible risk of transmitting
Cryptosporidium parvum infections to humans.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16905259</pmid><doi>10.1016/j.vetpar.2006.07.007</doi><tpages>7</tpages></addata></record> |
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| ispartof | Veterinary parasitology, 2006-12, Vol.142 (3), p.231-237 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Cattle Cattle Diseases - epidemiology Cattle Diseases - parasitology cryptosporidiosis Cryptosporidiosis - epidemiology Cryptosporidium Cryptosporidium - isolation & purification Cryptosporidium parvum dairy cows dairy herds disease prevalence feces Feces - parasitology Female genotype Genotyping Giardia - isolation & purification Giardia duodenalis Giardia lamblia giardiasis Giardiasis - epidemiology molecular systematics pathogen identification Polymerase Chain Reaction - veterinary Prevalence risk assessment Schools, Veterinary Time Factors veterinary education zoonoses Zoonoses - epidemiology Zoonoses - parasitology Zoonotic risk |
| title | Giardia duodenalis and Cryptosporidium spp. in a veterinary college bovine teaching herd |
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