Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection
We evaluated the cell wall binding (CWB) domain of Staphylococcus aureus autolysin as an affinity reagent for bacteria. A fusion of CWB domain and green fluorescent protein (CWB-GFP) bound to S. aureus with a dissociation constant of 15 nM. CWB-GFP bound to a wide range of gram-positive bacteria, bu...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2007-07, Vol.104 (1), p.55-61 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Ahmed, Abo Bakr F. Noguchi, Kanako Asami, Yasuo Nomura, Kazutaka Fujii, Hiroya Sakata, Minoru Tokita, Akihiko Noda, Kenichi Kuroda, Akio |
| description | We evaluated the cell wall binding (CWB) domain of
Staphylococcus aureus autolysin as an affinity reagent for bacteria. A fusion of CWB domain and green fluorescent protein (CWB-GFP) bound to
S. aureus with a dissociation constant of 15 nM. CWB-GFP bound to a wide range of gram-positive bacteria, but not to most gram-negative bacteria. We suspected that the outer membrane of gram-negative bacteria inhibits the access of CWB-GFP to peptidoglycan layer. Indeed, CWB-GFP bound to gram-negative bacteria when they were treated with benzalkonium chloride. Because CWB-GFP bound to the bacterial peptidoglycan layer, it appeared to be an effective affinity reagent for bacteria and CWB fusion with reporter proteins could be applied to detect bacteria. We also constructed a fusion of CWB and luciferase, which can be used for the rapid detection of bacteria. |
| doi_str_mv | 10.1263/jbb.104.55 |
| format | Article |
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Staphylococcus aureus autolysin as an affinity reagent for bacteria. A fusion of CWB domain and green fluorescent protein (CWB-GFP) bound to
S. aureus with a dissociation constant of 15 nM. CWB-GFP bound to a wide range of gram-positive bacteria, but not to most gram-negative bacteria. We suspected that the outer membrane of gram-negative bacteria inhibits the access of CWB-GFP to peptidoglycan layer. Indeed, CWB-GFP bound to gram-negative bacteria when they were treated with benzalkonium chloride. Because CWB-GFP bound to the bacterial peptidoglycan layer, it appeared to be an effective affinity reagent for bacteria and CWB fusion with reporter proteins could be applied to detect bacteria. We also constructed a fusion of CWB and luciferase, which can be used for the rapid detection of bacteria.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1263/jbb.104.55</identifier><identifier>PMID: 17697984</identifier><identifier>CODEN: JFBIEX</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>affinity reagent ; Bacteria - chemistry ; Bacteria - isolation & purification ; BACTERIA GRAM NEGATIVA ; BACTERIA GRAM POSITIVA ; bacterial probe ; BACTERIE GRAM NEGATIF ; BACTERIE GRAM POSITIF ; Bacteriological Techniques - methods ; BINDING PROTEINS ; Biological and medical sciences ; Biotechnology ; Cell Wall - chemistry ; cell wall binding ; CELL WALLS ; Fundamental and applied biological sciences. Psychology ; GRAM NEGATIVE BACTERIA ; GRAM POSITIVE BACTERIA ; Green Fluorescent Proteins - analysis ; Green Fluorescent Proteins - genetics ; Lactobacillus - chemistry ; Lactobacillus - isolation & purification ; Luciferases - analysis ; Luciferases - genetics ; microbe counting ; N-Acetylmuramoyl-L-alanine Amidase - analysis ; N-Acetylmuramoyl-L-alanine Amidase - chemistry ; N-Acetylmuramoyl-L-alanine Amidase - genetics ; PARED CELULAR ; PAROI CELLULAIRE ; Protein Structure, Tertiary ; PROTEINAS AGLUTINANTES ; PROTEINE DE LIAISON ; Recombinant Fusion Proteins - analysis ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Spores, Bacterial - chemistry ; Spores, Bacterial - isolation & purification ; STAPHYLOCOCCUS AUREUS ; Staphylococcus aureus - enzymology</subject><ispartof>Journal of bioscience and bioengineering, 2007-07, Vol.104 (1), p.55-61</ispartof><rights>2007 The Society for Biotechnology, Japan</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-f82c5b2001f0df34383d127210a99873d358eefdd191e6810b3b39e5cb38019c3</citedby><cites>FETCH-LOGICAL-c556t-f82c5b2001f0df34383d127210a99873d358eefdd191e6810b3b39e5cb38019c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172307701196$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18956978$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17697984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmed, Abo Bakr F.</creatorcontrib><creatorcontrib>Noguchi, Kanako</creatorcontrib><creatorcontrib>Asami, Yasuo</creatorcontrib><creatorcontrib>Nomura, Kazutaka</creatorcontrib><creatorcontrib>Fujii, Hiroya</creatorcontrib><creatorcontrib>Sakata, Minoru</creatorcontrib><creatorcontrib>Tokita, Akihiko</creatorcontrib><creatorcontrib>Noda, Kenichi</creatorcontrib><creatorcontrib>Kuroda, Akio</creatorcontrib><title>Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>We evaluated the cell wall binding (CWB) domain of
Staphylococcus aureus autolysin as an affinity reagent for bacteria. A fusion of CWB domain and green fluorescent protein (CWB-GFP) bound to
S. aureus with a dissociation constant of 15 nM. CWB-GFP bound to a wide range of gram-positive bacteria, but not to most gram-negative bacteria. We suspected that the outer membrane of gram-negative bacteria inhibits the access of CWB-GFP to peptidoglycan layer. Indeed, CWB-GFP bound to gram-negative bacteria when they were treated with benzalkonium chloride. Because CWB-GFP bound to the bacterial peptidoglycan layer, it appeared to be an effective affinity reagent for bacteria and CWB fusion with reporter proteins could be applied to detect bacteria. We also constructed a fusion of CWB and luciferase, which can be used for the rapid detection of bacteria.</description><subject>affinity reagent</subject><subject>Bacteria - chemistry</subject><subject>Bacteria - isolation & purification</subject><subject>BACTERIA GRAM NEGATIVA</subject><subject>BACTERIA GRAM POSITIVA</subject><subject>bacterial probe</subject><subject>BACTERIE GRAM NEGATIF</subject><subject>BACTERIE GRAM POSITIF</subject><subject>Bacteriological Techniques - methods</subject><subject>BINDING PROTEINS</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Wall - chemistry</subject><subject>cell wall binding</subject><subject>CELL WALLS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GRAM NEGATIVE BACTERIA</subject><subject>GRAM POSITIVE BACTERIA</subject><subject>Green Fluorescent Proteins - analysis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Lactobacillus - chemistry</subject><subject>Lactobacillus - isolation & purification</subject><subject>Luciferases - analysis</subject><subject>Luciferases - genetics</subject><subject>microbe counting</subject><subject>N-Acetylmuramoyl-L-alanine Amidase - analysis</subject><subject>N-Acetylmuramoyl-L-alanine Amidase - chemistry</subject><subject>N-Acetylmuramoyl-L-alanine Amidase - genetics</subject><subject>PARED CELULAR</subject><subject>PAROI CELLULAIRE</subject><subject>Protein Structure, Tertiary</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE DE LIAISON</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Spores, Bacterial - chemistry</subject><subject>Spores, Bacterial - isolation & purification</subject><subject>STAPHYLOCOCCUS AUREUS</subject><subject>Staphylococcus aureus - enzymology</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhhtR3A-9eFcCogdhxlQn6U6Oy7LrBwsK6jmk8zFm6OmMSXplfoj_15qdwQURvKSSvE8qVfU2zTOgS2g79nY9DEugfCnEg-YUGO8XnLfwcL-XagF9y06as1LWlEJPe3jcnEDfqV5Jftr8uro142xqTBNJgVg_juSnwWWIk4vTiri0MfFO-1LN9vtuTDZZOxdi5uzvQk3jriBi8BBCnGLdkezNyk-VhJTJYGz1ORpiJkdiRWq7HaM9_FnTH30kzldv99dPmkfBjMU_Pcbz5tv11dfL94ubT-8-XF7cLKwQXV0E2VoxtNhXoC4wziRz0PYtUKOU7JljQnofnAMFvpNABzYw5YUdmKSgLDtvXh_ybnP6MftS9SaW_QzM5NNcNL7peA_wXxBroExAh-DLv8B1mvOETWjgHFirVE-RenOgbE6lZB_0NseNyTsNVO891egp7rkWAuEXx5TzsPHuHj2aiMCrI2CKNWPIZrKx3HNSCSQlcs8PXDBJm1VG5uNnrFziAHnHUOcH3ePIb6PPutjoJ-tdzOiLdin-q77fnzfFqA</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Ahmed, Abo Bakr F.</creator><creator>Noguchi, Kanako</creator><creator>Asami, Yasuo</creator><creator>Nomura, Kazutaka</creator><creator>Fujii, Hiroya</creator><creator>Sakata, Minoru</creator><creator>Tokita, Akihiko</creator><creator>Noda, Kenichi</creator><creator>Kuroda, Akio</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection</title><author>Ahmed, Abo Bakr F. ; Noguchi, Kanako ; Asami, Yasuo ; Nomura, Kazutaka ; Fujii, Hiroya ; Sakata, Minoru ; Tokita, Akihiko ; Noda, Kenichi ; Kuroda, Akio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-f82c5b2001f0df34383d127210a99873d358eefdd191e6810b3b39e5cb38019c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>affinity reagent</topic><topic>Bacteria - chemistry</topic><topic>Bacteria - isolation & purification</topic><topic>BACTERIA GRAM NEGATIVA</topic><topic>BACTERIA GRAM POSITIVA</topic><topic>bacterial probe</topic><topic>BACTERIE GRAM NEGATIF</topic><topic>BACTERIE GRAM POSITIF</topic><topic>Bacteriological Techniques - methods</topic><topic>BINDING PROTEINS</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Wall - chemistry</topic><topic>cell wall binding</topic><topic>CELL WALLS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GRAM NEGATIVE BACTERIA</topic><topic>GRAM POSITIVE BACTERIA</topic><topic>Green Fluorescent Proteins - analysis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Lactobacillus - chemistry</topic><topic>Lactobacillus - isolation & purification</topic><topic>Luciferases - analysis</topic><topic>Luciferases - genetics</topic><topic>microbe counting</topic><topic>N-Acetylmuramoyl-L-alanine Amidase - analysis</topic><topic>N-Acetylmuramoyl-L-alanine Amidase - chemistry</topic><topic>N-Acetylmuramoyl-L-alanine Amidase - genetics</topic><topic>PARED CELULAR</topic><topic>PAROI CELLULAIRE</topic><topic>Protein Structure, Tertiary</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINE DE LIAISON</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Spores, Bacterial - chemistry</topic><topic>Spores, Bacterial - isolation & purification</topic><topic>STAPHYLOCOCCUS AUREUS</topic><topic>Staphylococcus aureus - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmed, Abo Bakr F.</creatorcontrib><creatorcontrib>Noguchi, Kanako</creatorcontrib><creatorcontrib>Asami, Yasuo</creatorcontrib><creatorcontrib>Nomura, Kazutaka</creatorcontrib><creatorcontrib>Fujii, Hiroya</creatorcontrib><creatorcontrib>Sakata, Minoru</creatorcontrib><creatorcontrib>Tokita, Akihiko</creatorcontrib><creatorcontrib>Noda, Kenichi</creatorcontrib><creatorcontrib>Kuroda, Akio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmed, Abo Bakr F.</au><au>Noguchi, Kanako</au><au>Asami, Yasuo</au><au>Nomura, Kazutaka</au><au>Fujii, Hiroya</au><au>Sakata, Minoru</au><au>Tokita, Akihiko</au><au>Noda, Kenichi</au><au>Kuroda, Akio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>104</volume><issue>1</issue><spage>55</spage><epage>61</epage><pages>55-61</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><coden>JFBIEX</coden><abstract>We evaluated the cell wall binding (CWB) domain of
Staphylococcus aureus autolysin as an affinity reagent for bacteria. A fusion of CWB domain and green fluorescent protein (CWB-GFP) bound to
S. aureus with a dissociation constant of 15 nM. CWB-GFP bound to a wide range of gram-positive bacteria, but not to most gram-negative bacteria. We suspected that the outer membrane of gram-negative bacteria inhibits the access of CWB-GFP to peptidoglycan layer. Indeed, CWB-GFP bound to gram-negative bacteria when they were treated with benzalkonium chloride. Because CWB-GFP bound to the bacterial peptidoglycan layer, it appeared to be an effective affinity reagent for bacteria and CWB fusion with reporter proteins could be applied to detect bacteria. We also constructed a fusion of CWB and luciferase, which can be used for the rapid detection of bacteria.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>17697984</pmid><doi>10.1263/jbb.104.55</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-1723 |
| ispartof | Journal of bioscience and bioengineering, 2007-07, Vol.104 (1), p.55-61 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | affinity reagent Bacteria - chemistry Bacteria - isolation & purification BACTERIA GRAM NEGATIVA BACTERIA GRAM POSITIVA bacterial probe BACTERIE GRAM NEGATIF BACTERIE GRAM POSITIF Bacteriological Techniques - methods BINDING PROTEINS Biological and medical sciences Biotechnology Cell Wall - chemistry cell wall binding CELL WALLS Fundamental and applied biological sciences. Psychology GRAM NEGATIVE BACTERIA GRAM POSITIVE BACTERIA Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Lactobacillus - chemistry Lactobacillus - isolation & purification Luciferases - analysis Luciferases - genetics microbe counting N-Acetylmuramoyl-L-alanine Amidase - analysis N-Acetylmuramoyl-L-alanine Amidase - chemistry N-Acetylmuramoyl-L-alanine Amidase - genetics PARED CELULAR PAROI CELLULAIRE Protein Structure, Tertiary PROTEINAS AGLUTINANTES PROTEINE DE LIAISON Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Spores, Bacterial - chemistry Spores, Bacterial - isolation & purification STAPHYLOCOCCUS AUREUS Staphylococcus aureus - enzymology |
| title | Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection |
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