Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay
Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2006-11, Vol.386 (6), p.1727-1733 |
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| container_issue | 6 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Liu, Nan Wen, Xingbing Liu, Jihua Liang, Ming Zeng, Huajin Lin, Yining Yu, Boyang |
| description | Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin. |
| doi_str_mv | 10.1007/s00216-006-0767-9 |
| format | Article |
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We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-006-0767-9</identifier><identifier>PMID: 16957914</identifier><language>eng</language><publisher>Germany: Springer-Verlag</publisher><subject>Animals ; Antibodies - blood ; Antibodies - immunology ; Antigens - immunology ; bioactive properties ; bovine serum albumin ; Chromatography, High Pressure Liquid ; cross reaction ; diosgenin ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Male ; Medicine, Chinese Traditional ; Molecular Structure ; Ophiopogon - chemistry ; Ophiopogon - immunology ; Ophiopogon japonicus ; Oriental traditional medicine ; pharmacokinetics ; Plant Extracts - chemistry ; Plant Extracts - immunology ; polyclonal antibodies ; quantitative analysis ; Rabbits ; Rats ; Sensitivity and Specificity ; serum albumin ; Spirostans - analysis ; Spirostans - chemistry ; Spirostans - immunology ; Spirostans - pharmacokinetics</subject><ispartof>Analytical and bioanalytical chemistry, 2006-11, Vol.386 (6), p.1727-1733</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-150e50b2b918598613f871a1c689aea8ede95bceea4819323af697aada96dd6e3</citedby><cites>FETCH-LOGICAL-c323t-150e50b2b918598613f871a1c689aea8ede95bceea4819323af697aada96dd6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16957914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Nan</creatorcontrib><creatorcontrib>Wen, Xingbing</creatorcontrib><creatorcontrib>Liu, Jihua</creatorcontrib><creatorcontrib>Liang, Ming</creatorcontrib><creatorcontrib>Zeng, Huajin</creatorcontrib><creatorcontrib>Lin, Yining</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><title>Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><description>Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.</description><subject>Animals</subject><subject>Antibodies - blood</subject><subject>Antibodies - immunology</subject><subject>Antigens - immunology</subject><subject>bioactive properties</subject><subject>bovine serum albumin</subject><subject>Chromatography, High Pressure Liquid</subject><subject>cross reaction</subject><subject>diosgenin</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Male</subject><subject>Medicine, Chinese Traditional</subject><subject>Molecular Structure</subject><subject>Ophiopogon - chemistry</subject><subject>Ophiopogon - immunology</subject><subject>Ophiopogon japonicus</subject><subject>Oriental traditional medicine</subject><subject>pharmacokinetics</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - immunology</subject><subject>polyclonal antibodies</subject><subject>quantitative analysis</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><subject>serum albumin</subject><subject>Spirostans - analysis</subject><subject>Spirostans - chemistry</subject><subject>Spirostans - immunology</subject><subject>Spirostans - pharmacokinetics</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU9PwzAMxSMEYmPwAbhATtwKcbumzRGNv9IkDrAbUuSm7ghqm5Gsh317MnUCKZYt670n-RfGLkHcghDFXRAiBZkIEauQRaKO2BQklEkqc3H8N8_TCTsL4VsIyEuQp2wCUuWFgvmUfT7Qlnxne9xa13PXcD8E49bU257HZ_xQE1982Z4C8Y5qa_Yjx77mlXWtW1uDLQ_Ybdq4rnbcdt3QOwwBd-fspME20MWhz9jq6fFj8ZIs355fF_fLxGRptk0gF5SLKq0UlLkqJWRNWQCCkaVCwpJqUnlliHBegooWbKQqEGtUsq4lZTN2M-ZuvPsZKGx1Z4OhtsWe3BC0jFdnEtIohFFovAvBU6M33nbodxqE3iPVI1Idkeo9Uq2i5-oQPlTx_n_HgWEUXI-CBp3GtbdBr95TAVLErPgDKvsFPkR8TQ</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Liu, Nan</creator><creator>Wen, Xingbing</creator><creator>Liu, Jihua</creator><creator>Liang, Ming</creator><creator>Zeng, Huajin</creator><creator>Lin, Yining</creator><creator>Yu, Boyang</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061101</creationdate><title>Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay</title><author>Liu, Nan ; Wen, Xingbing ; Liu, Jihua ; Liang, Ming ; Zeng, Huajin ; Lin, Yining ; Yu, Boyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-150e50b2b918598613f871a1c689aea8ede95bceea4819323af697aada96dd6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antibodies - blood</topic><topic>Antibodies - immunology</topic><topic>Antigens - immunology</topic><topic>bioactive properties</topic><topic>bovine serum albumin</topic><topic>Chromatography, High Pressure Liquid</topic><topic>cross reaction</topic><topic>diosgenin</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Male</topic><topic>Medicine, Chinese Traditional</topic><topic>Molecular Structure</topic><topic>Ophiopogon - chemistry</topic><topic>Ophiopogon - immunology</topic><topic>Ophiopogon japonicus</topic><topic>Oriental traditional medicine</topic><topic>pharmacokinetics</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - immunology</topic><topic>polyclonal antibodies</topic><topic>quantitative analysis</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><topic>serum albumin</topic><topic>Spirostans - analysis</topic><topic>Spirostans - chemistry</topic><topic>Spirostans - immunology</topic><topic>Spirostans - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Nan</creatorcontrib><creatorcontrib>Wen, Xingbing</creatorcontrib><creatorcontrib>Liu, Jihua</creatorcontrib><creatorcontrib>Liang, Ming</creatorcontrib><creatorcontrib>Zeng, Huajin</creatorcontrib><creatorcontrib>Lin, Yining</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Nan</au><au>Wen, Xingbing</au><au>Liu, Jihua</au><au>Liang, Ming</au><au>Zeng, Huajin</au><au>Lin, Yining</au><au>Yu, Boyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><addtitle>Anal Bioanal Chem</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>386</volume><issue>6</issue><spage>1727</spage><epage>1733</epage><pages>1727-1733</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.</abstract><cop>Germany</cop><pub>Springer-Verlag</pub><pmid>16957914</pmid><doi>10.1007/s00216-006-0767-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2006-11, Vol.386 (6), p.1727-1733 |
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| subjects | Animals Antibodies - blood Antibodies - immunology Antigens - immunology bioactive properties bovine serum albumin Chromatography, High Pressure Liquid cross reaction diosgenin enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Female Male Medicine, Chinese Traditional Molecular Structure Ophiopogon - chemistry Ophiopogon - immunology Ophiopogon japonicus Oriental traditional medicine pharmacokinetics Plant Extracts - chemistry Plant Extracts - immunology polyclonal antibodies quantitative analysis Rabbits Rats Sensitivity and Specificity serum albumin Spirostans - analysis Spirostans - chemistry Spirostans - immunology Spirostans - pharmacokinetics |
| title | Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay |
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