Folding and assembly of co-chaperonin heptamer probed by forster resonance energy transfer

The ring-shaped heptameric co-chaperonin protein 10 (cpn10) is one of few oligomeric β-sheet proteins that unfold and disassemble reversibly in vitro. Here, we labeled human mitochondrial cpn10 with donor and acceptor dyes to obtain FRET signals. Cpn10 mixed in a 1:1:5 ratio of donor:acceptor:unlabe...

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