Isolation and Comparative Study of Cell-Free Nucleic Acids from Human Urine
: Cell‐free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cel...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2006-09, Vol.1075 (1), p.334-340 |
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| creator | BRYZGUNOVA, OLGA E. SKVORTSOVA, TATYANA E. KOLESNIKOVA, ELENA V. STARIKOV, ANDREY V. RYKOVA, ELENA YU VLASSOV, VALENTIN V. LAKTIONOV, PAVEL P. |
| description | : Cell‐free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cell‐free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell‐free DNA isolated from their urine by methylation‐specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARβ2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell‐free urine DNA in cancer diagnostics. |
| doi_str_mv | 10.1196/annals.1368.045 |
| format | Article |
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Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cell‐free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell‐free DNA isolated from their urine by methylation‐specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARβ2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell‐free urine DNA in cancer diagnostics.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1196/annals.1368.045</identifier><identifier>PMID: 17108229</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - urine ; blood ; breast cancer ; Breast Neoplasms - blood ; Breast Neoplasms - diagnosis ; Breast Neoplasms - genetics ; Breast Neoplasms - urine ; cell-free DNA ; cell-free RNA ; DNA Fragmentation ; DNA Methylation ; Female ; Humans ; Male ; methylation-specific PCR ; Molecular Diagnostic Techniques ; Nucleic Acids - blood ; Nucleic Acids - isolation & purification ; Nucleic Acids - urine ; Promoter Regions, Genetic ; Receptors, Retinoic Acid - genetics ; Receptors, Retinoic Acid - metabolism ; Tumor Suppressor Proteins - blood ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - urine ; urine</subject><ispartof>Annals of the New York Academy of Sciences, 2006-09, Vol.1075 (1), p.334-340</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5055-4e608aad5e907b5f43977b6ded2d74b3225cf55eb47bdcbcfdacbd19cdcc3c2d3</citedby><cites>FETCH-LOGICAL-c5055-4e608aad5e907b5f43977b6ded2d74b3225cf55eb47bdcbcfdacbd19cdcc3c2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1368.045$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1368.045$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17108229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRYZGUNOVA, OLGA E.</creatorcontrib><creatorcontrib>SKVORTSOVA, TATYANA E.</creatorcontrib><creatorcontrib>KOLESNIKOVA, ELENA V.</creatorcontrib><creatorcontrib>STARIKOV, ANDREY V.</creatorcontrib><creatorcontrib>RYKOVA, ELENA YU</creatorcontrib><creatorcontrib>VLASSOV, VALENTIN V.</creatorcontrib><creatorcontrib>LAKTIONOV, PAVEL P.</creatorcontrib><title>Isolation and Comparative Study of Cell-Free Nucleic Acids from Human Urine</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: Cell‐free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cell‐free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell‐free DNA isolated from their urine by methylation‐specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARβ2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell‐free urine DNA in cancer diagnostics.</description><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - urine</subject><subject>blood</subject><subject>breast cancer</subject><subject>Breast Neoplasms - blood</subject><subject>Breast Neoplasms - diagnosis</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - urine</subject><subject>cell-free DNA</subject><subject>cell-free RNA</subject><subject>DNA Fragmentation</subject><subject>DNA Methylation</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>methylation-specific PCR</subject><subject>Molecular Diagnostic Techniques</subject><subject>Nucleic Acids - blood</subject><subject>Nucleic Acids - isolation & purification</subject><subject>Nucleic Acids - urine</subject><subject>Promoter Regions, Genetic</subject><subject>Receptors, Retinoic Acid - genetics</subject><subject>Receptors, Retinoic Acid - metabolism</subject><subject>Tumor Suppressor Proteins - blood</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - urine</subject><subject>urine</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPGzEUha2qqISUdXeVV91N4rfHyygqAQFBCChiZXnsO9K080jtDG3-PQMTwTKrqyt95-hIH0LfKJlRatTcta2r04xylc-IkJ_QhGphMqU4-4wmhGid5YbxY3SS0m9CKMuF_oKOqaYkZ8xM0OVF6mq3rboWuzbgZddsXBz-Z8B32z7scFfiJdR1dhYB8Lr3NVQeL3wVEi5j1-DzvnEtfohVC1_RUTmsgdP9naKHs5_3y_Ps6mZ1sVxcZV4SKTMBiuTOBQmG6EKWghutCxUgsKBFwRmTvpQSCqGL4AtfBueLQI0P3nPPAp-iH2PvJnZ_e0hb21TJDyNdC12frMqppESQgyAjmnFDxUGQGqEYZ_kAzkfQxy6lCKXdxKpxcWcpsa9G7GjEvhqxg5Eh8X1f3RcNhA9-r2AA-Aj8q2rYHeqz66fF3VttNqaqtIX_7ykX_1iluZb2cb2yt78ofbzO7-2KvwCZ7qf5</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>BRYZGUNOVA, OLGA E.</creator><creator>SKVORTSOVA, TATYANA E.</creator><creator>KOLESNIKOVA, ELENA V.</creator><creator>STARIKOV, ANDREY V.</creator><creator>RYKOVA, ELENA YU</creator><creator>VLASSOV, VALENTIN V.</creator><creator>LAKTIONOV, PAVEL P.</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Isolation and Comparative Study of Cell-Free Nucleic Acids from Human Urine</title><author>BRYZGUNOVA, OLGA E. ; SKVORTSOVA, TATYANA E. ; KOLESNIKOVA, ELENA V. ; STARIKOV, ANDREY V. ; RYKOVA, ELENA YU ; VLASSOV, VALENTIN V. ; LAKTIONOV, PAVEL P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5055-4e608aad5e907b5f43977b6ded2d74b3225cf55eb47bdcbcfdacbd19cdcc3c2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - urine</topic><topic>blood</topic><topic>breast cancer</topic><topic>Breast Neoplasms - blood</topic><topic>Breast Neoplasms - diagnosis</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - urine</topic><topic>cell-free DNA</topic><topic>cell-free RNA</topic><topic>DNA Fragmentation</topic><topic>DNA Methylation</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>methylation-specific PCR</topic><topic>Molecular Diagnostic Techniques</topic><topic>Nucleic Acids - blood</topic><topic>Nucleic Acids - isolation & purification</topic><topic>Nucleic Acids - urine</topic><topic>Promoter Regions, Genetic</topic><topic>Receptors, Retinoic Acid - genetics</topic><topic>Receptors, Retinoic Acid - metabolism</topic><topic>Tumor Suppressor Proteins - blood</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - urine</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRYZGUNOVA, OLGA E.</creatorcontrib><creatorcontrib>SKVORTSOVA, TATYANA E.</creatorcontrib><creatorcontrib>KOLESNIKOVA, ELENA V.</creatorcontrib><creatorcontrib>STARIKOV, ANDREY V.</creatorcontrib><creatorcontrib>RYKOVA, ELENA YU</creatorcontrib><creatorcontrib>VLASSOV, VALENTIN V.</creatorcontrib><creatorcontrib>LAKTIONOV, PAVEL P.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRYZGUNOVA, OLGA E.</au><au>SKVORTSOVA, TATYANA E.</au><au>KOLESNIKOVA, ELENA V.</au><au>STARIKOV, ANDREY V.</au><au>RYKOVA, ELENA YU</au><au>VLASSOV, VALENTIN V.</au><au>LAKTIONOV, PAVEL P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Comparative Study of Cell-Free Nucleic Acids from Human Urine</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2006-09</date><risdate>2006</risdate><volume>1075</volume><issue>1</issue><spage>334</spage><epage>340</epage><pages>334-340</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: Cell‐free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cell‐free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell‐free DNA isolated from their urine by methylation‐specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARβ2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell‐free urine DNA in cancer diagnostics.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>17108229</pmid><doi>10.1196/annals.1368.045</doi><tpages>7</tpages></addata></record> |
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| subjects | Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Biomarkers, Tumor - urine blood breast cancer Breast Neoplasms - blood Breast Neoplasms - diagnosis Breast Neoplasms - genetics Breast Neoplasms - urine cell-free DNA cell-free RNA DNA Fragmentation DNA Methylation Female Humans Male methylation-specific PCR Molecular Diagnostic Techniques Nucleic Acids - blood Nucleic Acids - isolation & purification Nucleic Acids - urine Promoter Regions, Genetic Receptors, Retinoic Acid - genetics Receptors, Retinoic Acid - metabolism Tumor Suppressor Proteins - blood Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - urine urine |
| title | Isolation and Comparative Study of Cell-Free Nucleic Acids from Human Urine |
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