Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma
: Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methyl...
Gespeichert in:
| Veröffentlicht in: | Annals of the New York Academy of Sciences 2006-09, Vol.1075 (1), p.179-184 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 184 |
|---|---|
| container_issue | 1 |
| container_start_page | 179 |
| container_title | Annals of the New York Academy of Sciences |
| container_volume | 1075 |
| creator | WANG, YI-CHING HSU, HAN-SHUI CHEN, TSZ-PEI CHEN, JUNG-TA |
| description | : Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation‐specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection. |
| doi_str_mv | 10.1196/annals.1368.024 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68151006</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68151006</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3774-820c51d256f190ed5d9ab6beda92e38e5db499de10412c45532303a5eec989c13</originalsourceid><addsrcrecordid>eNqFkEtPAjEURhujEUTX7kxX7gb7mLbTJaKiCaAJGnTVlM7FjM4DWybKv3dwiC5ddXO-c5uD0CklfUq1vLBlafPQp1wmfcLiPdSlKtaRlJztoy4hSkWJZryDjkJ4I4SyJFaHqEMVJQkjuosGkyoHV-fW46vMvpZVWGcOT6x_Bx_wsvJ4XJeveGhLBx5nJZ6t6nVdYFum-CG3obDH6GDZ_AFOdm8PPd1cPw5vo_H96G44GEeOKxVHzTknaMqEXFJNIBWptgu5gNRqBjwBkS5irVOgJKbMxUJwxgm3AsDpRDvKe-i89a589VFDWJsiCw7y3JZQ1cHIhApKiGzAixZ0vgrBw9KsfFZYvzGUmG0101Yz22qmqdYsznbqelFA-sfvMjUAb4HPLIfNfz4zfRnMfrRRu8rCGr5-V01bIxVXwsynIzMa3kwup88jM-ffcH2INw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68151006</pqid></control><display><type>article</type><title>Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma</title><source>MEDLINE</source><source>Wiley Journals</source><creator>WANG, YI-CHING ; HSU, HAN-SHUI ; CHEN, TSZ-PEI ; CHEN, JUNG-TA</creator><creatorcontrib>WANG, YI-CHING ; HSU, HAN-SHUI ; CHEN, TSZ-PEI ; CHEN, JUNG-TA</creatorcontrib><description>: Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation‐specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1196/annals.1368.024</identifier><identifier>PMID: 17108209</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>biomarker ; Biomarkers, Tumor - analysis ; DNA Methylation ; DNA, Neoplasm - analysis ; Humans ; LOH ; Loss of Heterozygosity ; Lung Neoplasms - diagnosis ; Lung Neoplasms - genetics ; Microsatellite Instability ; Microsatellite Repeats ; MSI ; NSCLC ; plasma ; Plasma - chemistry ; promoter hypermethylation ; Sensitivity and Specificity ; sputum ; Sputum - chemistry</subject><ispartof>Annals of the New York Academy of Sciences, 2006-09, Vol.1075 (1), p.179-184</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3774-820c51d256f190ed5d9ab6beda92e38e5db499de10412c45532303a5eec989c13</citedby><cites>FETCH-LOGICAL-c3774-820c51d256f190ed5d9ab6beda92e38e5db499de10412c45532303a5eec989c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1368.024$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1368.024$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17108209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WANG, YI-CHING</creatorcontrib><creatorcontrib>HSU, HAN-SHUI</creatorcontrib><creatorcontrib>CHEN, TSZ-PEI</creatorcontrib><creatorcontrib>CHEN, JUNG-TA</creatorcontrib><title>Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation‐specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.</description><subject>biomarker</subject><subject>Biomarkers, Tumor - analysis</subject><subject>DNA Methylation</subject><subject>DNA, Neoplasm - analysis</subject><subject>Humans</subject><subject>LOH</subject><subject>Loss of Heterozygosity</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lung Neoplasms - genetics</subject><subject>Microsatellite Instability</subject><subject>Microsatellite Repeats</subject><subject>MSI</subject><subject>NSCLC</subject><subject>plasma</subject><subject>Plasma - chemistry</subject><subject>promoter hypermethylation</subject><subject>Sensitivity and Specificity</subject><subject>sputum</subject><subject>Sputum - chemistry</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPAjEURhujEUTX7kxX7gb7mLbTJaKiCaAJGnTVlM7FjM4DWybKv3dwiC5ddXO-c5uD0CklfUq1vLBlafPQp1wmfcLiPdSlKtaRlJztoy4hSkWJZryDjkJ4I4SyJFaHqEMVJQkjuosGkyoHV-fW46vMvpZVWGcOT6x_Bx_wsvJ4XJeveGhLBx5nJZ6t6nVdYFum-CG3obDH6GDZ_AFOdm8PPd1cPw5vo_H96G44GEeOKxVHzTknaMqEXFJNIBWptgu5gNRqBjwBkS5irVOgJKbMxUJwxgm3AsDpRDvKe-i89a589VFDWJsiCw7y3JZQ1cHIhApKiGzAixZ0vgrBw9KsfFZYvzGUmG0101Yz22qmqdYsznbqelFA-sfvMjUAb4HPLIfNfz4zfRnMfrRRu8rCGr5-V01bIxVXwsynIzMa3kwup88jM-ffcH2INw</recordid><startdate>200609</startdate><enddate>200609</enddate><creator>WANG, YI-CHING</creator><creator>HSU, HAN-SHUI</creator><creator>CHEN, TSZ-PEI</creator><creator>CHEN, JUNG-TA</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200609</creationdate><title>Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma</title><author>WANG, YI-CHING ; HSU, HAN-SHUI ; CHEN, TSZ-PEI ; CHEN, JUNG-TA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3774-820c51d256f190ed5d9ab6beda92e38e5db499de10412c45532303a5eec989c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>biomarker</topic><topic>Biomarkers, Tumor - analysis</topic><topic>DNA Methylation</topic><topic>DNA, Neoplasm - analysis</topic><topic>Humans</topic><topic>LOH</topic><topic>Loss of Heterozygosity</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Lung Neoplasms - genetics</topic><topic>Microsatellite Instability</topic><topic>Microsatellite Repeats</topic><topic>MSI</topic><topic>NSCLC</topic><topic>plasma</topic><topic>Plasma - chemistry</topic><topic>promoter hypermethylation</topic><topic>Sensitivity and Specificity</topic><topic>sputum</topic><topic>Sputum - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, YI-CHING</creatorcontrib><creatorcontrib>HSU, HAN-SHUI</creatorcontrib><creatorcontrib>CHEN, TSZ-PEI</creatorcontrib><creatorcontrib>CHEN, JUNG-TA</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, YI-CHING</au><au>HSU, HAN-SHUI</au><au>CHEN, TSZ-PEI</au><au>CHEN, JUNG-TA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2006-09</date><risdate>2006</risdate><volume>1075</volume><issue>1</issue><spage>179</spage><epage>184</epage><pages>179-184</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation‐specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>17108209</pmid><doi>10.1196/annals.1368.024</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0077-8923 |
| ispartof | Annals of the New York Academy of Sciences, 2006-09, Vol.1075 (1), p.179-184 |
| issn | 0077-8923 1749-6632 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68151006 |
| source | MEDLINE; Wiley Journals |
| subjects | biomarker Biomarkers, Tumor - analysis DNA Methylation DNA, Neoplasm - analysis Humans LOH Loss of Heterozygosity Lung Neoplasms - diagnosis Lung Neoplasms - genetics Microsatellite Instability Microsatellite Repeats MSI NSCLC plasma Plasma - chemistry promoter hypermethylation Sensitivity and Specificity sputum Sputum - chemistry |
| title | Molecular Diagnostic Markers for Lung Cancer in Sputum and Plasma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T12%3A41%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Diagnostic%20Markers%20for%20Lung%20Cancer%20in%20Sputum%20and%20Plasma&rft.jtitle=Annals%20of%20the%20New%20York%20Academy%20of%20Sciences&rft.au=WANG,%20YI-CHING&rft.date=2006-09&rft.volume=1075&rft.issue=1&rft.spage=179&rft.epage=184&rft.pages=179-184&rft.issn=0077-8923&rft.eissn=1749-6632&rft_id=info:doi/10.1196/annals.1368.024&rft_dat=%3Cproquest_cross%3E68151006%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68151006&rft_id=info:pmid/17108209&rfr_iscdi=true |