A Cellular FRET-Based Sensor for β-O-GlcNAc, A Dynamic Carbohydrate Modification Involved in Signaling

β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and...

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Veröffentlicht in:	Journal of the American Chemical Society 2006-11, Vol.128 (46), p.14768-14769
Hauptverfasser:	Carrillo, Luz D, Krishnamoorthy, Lakshmipriya, Mahal, Lara K
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - analysis Blotting, Western Carbohydrates - chemical synthesis Carbohydrates with 4, 5, 6, ... C atoms, dissacharides and oligosaccharides Carbohydrates. Nucleosides and nucleotides Chemistry Exact sciences and technology Fluorescence Resonance Energy Transfer - instrumentation General and physical chemistry HeLa Cells Humans Organic chemistry Preparations and properties Signal Transduction
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container_issue	46
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container_title	Journal of the American Chemical Society
container_volume	128
creator	Carrillo, Luz D Krishnamoorthy, Lakshmipriya Mahal, Lara K
description	β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.
doi_str_mv	10.1021/ja065835+
format	Article
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subjects	Acetylglucosamine - analysis Blotting, Western Carbohydrates - chemical synthesis Carbohydrates with 4, 5, 6, ... C atoms, dissacharides and oligosaccharides Carbohydrates. Nucleosides and nucleotides Chemistry Exact sciences and technology Fluorescence Resonance Energy Transfer - instrumentation General and physical chemistry HeLa Cells Humans Organic chemistry Preparations and properties Signal Transduction
title	A Cellular FRET-Based Sensor for β-O-GlcNAc, A Dynamic Carbohydrate Modification Involved in Signaling
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