Isolation of transcripts over-expressed in human pathogen Trichophyton rubrum during growth in keratin
Trichophyton rubrum is a cosmopolitan and anthropophilic fungus able to invade keratinized tissue, causing infection in human skin and nails. This work evaluated the changes in the extracellular pH during its growth in keratin (after 6, 12, 24, 48, 72 h and 7 days) at initial pH 5.0. We observed a g...
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| Veröffentlicht in: | Microbial pathogenesis 2007-10, Vol.43 (4), p.166-172 |
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| creator | Maranhao, FCA Paiao, F G Martinez-Rossi, N M |
| description | Trichophyton rubrum is a cosmopolitan and anthropophilic fungus able to invade keratinized tissue, causing infection in human skin and nails. This work evaluated the changes in the extracellular pH during its growth in keratin (after 6, 12, 24, 48, 72
h and 7 days) at initial pH 5.0. We observed a gradual increase of basal pH under keratin exposure when compared to glucose condition. Also, we identified 576
T. rubrum transcripts differentially expressed by subtractive suppression hybridization (SSH) using conidia cultivated for 72
h in keratin as tester, and cultivated in glucose as driver. The over-expression of 238 transcripts obtained under keratin condition was confirmed by macro-array dot-blot, revealing 28 unigenes. Putative proteins encoded by these genes showed similarity to fungi proteins involved in basic metabolism, growth and virulence, i.e., transporters ABC-MDR, MFS and ATPase of copper, NIMA interactive protein, Gag-Pol polyprotein, virulence factors serine-protease subtilisin and metalloprotease, cytochrome P450, GlcN-6-phosphate deaminase and Hsp30. The upregulation of
T. rubrum genes encoding subtilisin, metalloprotease and Gag-Pol polyprotein was also validated by northern blot. The results of this study provide the first insight into genes differentially expressed during
T. rubrum grown in keratin that may be involved in fungal pathogenesis. |
| doi_str_mv | 10.1016/j.micpath.2007.05.006 |
| format | Article |
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h and 7 days) at initial pH 5.0. We observed a gradual increase of basal pH under keratin exposure when compared to glucose condition. Also, we identified 576
T. rubrum transcripts differentially expressed by subtractive suppression hybridization (SSH) using conidia cultivated for 72
h in keratin as tester, and cultivated in glucose as driver. The over-expression of 238 transcripts obtained under keratin condition was confirmed by macro-array dot-blot, revealing 28 unigenes. Putative proteins encoded by these genes showed similarity to fungi proteins involved in basic metabolism, growth and virulence, i.e., transporters ABC-MDR, MFS and ATPase of copper, NIMA interactive protein, Gag-Pol polyprotein, virulence factors serine-protease subtilisin and metalloprotease, cytochrome P450, GlcN-6-phosphate deaminase and Hsp30. The upregulation of
T. rubrum genes encoding subtilisin, metalloprotease and Gag-Pol polyprotein was also validated by northern blot. The results of this study provide the first insight into genes differentially expressed during
T. rubrum grown in keratin that may be involved in fungal pathogenesis.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2007.05.006</identifier><identifier>PMID: 17590307</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Base Sequence ; Biological and medical sciences ; Blotting, Northern - methods ; Dermatophyte ; Female ; Fundamental and applied biological sciences. Psychology ; Human mycoses ; Humans ; Hydrogen-Ion Concentration ; Infectious diseases ; Keratin ; Keratins - metabolism ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Mycology ; Mycoses ; Mycoses of the skin ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; RNA, Messenger - isolation & purification ; SSH ; Tinea - microbiology ; Trichophyton - genetics ; Trichophyton - growth & development ; Trichophyton - isolation & purification ; Trichophyton - metabolism ; Trichophyton rubrum ; Virulence</subject><ispartof>Microbial pathogenesis, 2007-10, Vol.43 (4), p.166-172</ispartof><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-c19bb5bb6d3fabc13bf54b615128871979927a4f283cbabb799f16b8a2786ec63</citedby><cites>FETCH-LOGICAL-c490t-c19bb5bb6d3fabc13bf54b615128871979927a4f283cbabb799f16b8a2786ec63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2007.05.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19002203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17590307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maranhao, FCA</creatorcontrib><creatorcontrib>Paiao, F G</creatorcontrib><creatorcontrib>Martinez-Rossi, N M</creatorcontrib><title>Isolation of transcripts over-expressed in human pathogen Trichophyton rubrum during growth in keratin</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Trichophyton rubrum is a cosmopolitan and anthropophilic fungus able to invade keratinized tissue, causing infection in human skin and nails. This work evaluated the changes in the extracellular pH during its growth in keratin (after 6, 12, 24, 48, 72
h and 7 days) at initial pH 5.0. We observed a gradual increase of basal pH under keratin exposure when compared to glucose condition. Also, we identified 576
T. rubrum transcripts differentially expressed by subtractive suppression hybridization (SSH) using conidia cultivated for 72
h in keratin as tester, and cultivated in glucose as driver. The over-expression of 238 transcripts obtained under keratin condition was confirmed by macro-array dot-blot, revealing 28 unigenes. Putative proteins encoded by these genes showed similarity to fungi proteins involved in basic metabolism, growth and virulence, i.e., transporters ABC-MDR, MFS and ATPase of copper, NIMA interactive protein, Gag-Pol polyprotein, virulence factors serine-protease subtilisin and metalloprotease, cytochrome P450, GlcN-6-phosphate deaminase and Hsp30. The upregulation of
T. rubrum genes encoding subtilisin, metalloprotease and Gag-Pol polyprotein was also validated by northern blot. The results of this study provide the first insight into genes differentially expressed during
T. rubrum grown in keratin that may be involved in fungal pathogenesis.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern - methods</subject><subject>Dermatophyte</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human mycoses</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infectious diseases</subject><subject>Keratin</subject><subject>Keratins - metabolism</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mycology</subject><subject>Mycoses</subject><subject>Mycoses of the skin</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - isolation & purification</subject><subject>SSH</subject><subject>Tinea - microbiology</subject><subject>Trichophyton - genetics</subject><subject>Trichophyton - growth & development</subject><subject>Trichophyton - isolation & purification</subject><subject>Trichophyton - metabolism</subject><subject>Trichophyton rubrum</subject><subject>Virulence</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQhS0EopfCTwBlA7uEcR5-rBCqaKlUiU1ZW7Zj3_iS2MFOWvrvcXQjddnVaKTvzBmdg9BHDBUGTL6eqsnpWS5DVQPQCroKgLxCBwyclLgG9hodgLG6bAHDBXqX0gkAeNvwt-gC045DA_SA7G0Ko1xc8EWwxRKlTzq6eUlFeDCxNP_maFIyfeF8MayT9MXmGY7GF_fR6SHMw9OSxXFVcZ2Kfo3OH4tjDI_LsGn-mJiv-_fojZVjMh_2eYl-X_-4v_pZ3v26ub36flfqlsNSasyV6pQifWOl0rhRtmsVwR2uGaOYU85rKltbs0YrqVTeLSaKyZoyYjRpLtGX8905hr-rSYuYXNJmHKU3YU2CMNwyTtmLYPZqKG-aDHZnUMeQUjRWzNFNMj4JDGJrQpzE3oTYmhDQidxE1n3aDVY1mf5ZtUefgc87IJOWo83Ra5eeOQ5Q17A98O3MmZzbgzNRJO2M16Z30ehF9MG98Mp_dWyrvA</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Maranhao, FCA</creator><creator>Paiao, F G</creator><creator>Martinez-Rossi, N M</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Isolation of transcripts over-expressed in human pathogen Trichophyton rubrum during growth in keratin</title><author>Maranhao, FCA ; Paiao, F G ; Martinez-Rossi, N M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-c19bb5bb6d3fabc13bf54b615128871979927a4f283cbabb799f16b8a2786ec63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern - methods</topic><topic>Dermatophyte</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human mycoses</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infectious diseases</topic><topic>Keratin</topic><topic>Keratins - metabolism</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mycology</topic><topic>Mycoses</topic><topic>Mycoses of the skin</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - isolation & purification</topic><topic>SSH</topic><topic>Tinea - microbiology</topic><topic>Trichophyton - genetics</topic><topic>Trichophyton - growth & development</topic><topic>Trichophyton - isolation & purification</topic><topic>Trichophyton - metabolism</topic><topic>Trichophyton rubrum</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maranhao, FCA</creatorcontrib><creatorcontrib>Paiao, F G</creatorcontrib><creatorcontrib>Martinez-Rossi, N M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maranhao, FCA</au><au>Paiao, F G</au><au>Martinez-Rossi, N M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of transcripts over-expressed in human pathogen Trichophyton rubrum during growth in keratin</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>43</volume><issue>4</issue><spage>166</spage><epage>172</epage><pages>166-172</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>Trichophyton rubrum is a cosmopolitan and anthropophilic fungus able to invade keratinized tissue, causing infection in human skin and nails. This work evaluated the changes in the extracellular pH during its growth in keratin (after 6, 12, 24, 48, 72
h and 7 days) at initial pH 5.0. We observed a gradual increase of basal pH under keratin exposure when compared to glucose condition. Also, we identified 576
T. rubrum transcripts differentially expressed by subtractive suppression hybridization (SSH) using conidia cultivated for 72
h in keratin as tester, and cultivated in glucose as driver. The over-expression of 238 transcripts obtained under keratin condition was confirmed by macro-array dot-blot, revealing 28 unigenes. Putative proteins encoded by these genes showed similarity to fungi proteins involved in basic metabolism, growth and virulence, i.e., transporters ABC-MDR, MFS and ATPase of copper, NIMA interactive protein, Gag-Pol polyprotein, virulence factors serine-protease subtilisin and metalloprotease, cytochrome P450, GlcN-6-phosphate deaminase and Hsp30. The upregulation of
T. rubrum genes encoding subtilisin, metalloprotease and Gag-Pol polyprotein was also validated by northern blot. The results of this study provide the first insight into genes differentially expressed during
T. rubrum grown in keratin that may be involved in fungal pathogenesis.</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>17590307</pmid><doi>10.1016/j.micpath.2007.05.006</doi><tpages>7</tpages></addata></record> |
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| subjects | Base Sequence Biological and medical sciences Blotting, Northern - methods Dermatophyte Female Fundamental and applied biological sciences. Psychology Human mycoses Humans Hydrogen-Ion Concentration Infectious diseases Keratin Keratins - metabolism Medical sciences Microbiology Miscellaneous Molecular Sequence Data Mycology Mycoses Mycoses of the skin RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Messenger - isolation & purification SSH Tinea - microbiology Trichophyton - genetics Trichophyton - growth & development Trichophyton - isolation & purification Trichophyton - metabolism Trichophyton rubrum Virulence |
| title | Isolation of transcripts over-expressed in human pathogen Trichophyton rubrum during growth in keratin |
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