Multicenter Study on the Diagnostic Value of a New RIA for the Detection of Free Plasma Metanephrines in the Work-Up for Pheochromocytoma
: Available laboratory test methods for the detection of elevated concentrations of catecholamines and their metabolites in urine and/or plasma are not always sensitive enough for the detection of pheochromocytoma. High‐quality immunoassays for these compounds appear to be as accurate as high‐press...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2006-08, Vol.1073 (1), p.358-373 |
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| creator | LENZ, TOMAS ZORNER, JOACHIM KIRCHMAIER, CARL PILLITTERI, DANIELE BADENHOOP, KLAUS BARTEL, CHRISTINE GEIGER, HELMUT HASSELBACHER, KATRIN TUSCHY, ULRICH WESTERMANN, JÜRGEN SALEWSKI, LOTHAR |
| description | : Available laboratory test methods for the detection of elevated concentrations of catecholamines and their metabolites in urine and/or plasma are not always sensitive enough for the detection of pheochromocytoma. High‐quality immunoassays for these compounds appear to be as accurate as high‐pressure liquid chromatography (HPLC) or gas chromatography/mass spectrometry (GC‐MS). Therefore, the current project aims to establish a new sensitive radioimmunoassay (RIA) for the measurement of free metanephrines in the plasma of patients in the work‐up for pheochromocytoma. We report first results of an ongoing multicenter clinico–chemical evaluation study in hypertensive patients and normotensive volunteers. After an overnight fast plasma samples were collected on ice in EDTA‐ and heparin‐coated tubes after insertion of an indwelling venous line and resting in the supine (patients) or sitting position (normal volunteers) for 30 min. Plasma metanephrines were measured by a newly developed RIA from IBL, Hamburg, Germany. Good agreement of the assay with the tandem mass spectrometry (LC‐MS/MS) method for normetanephrine (r2= 0.975) and for metanephrine (r2= 0.985) could be demonstrated. Both specimens, EDTA and heparin plasma, can be used with the same results. The RIA has a good precision of |
| doi_str_mv | 10.1196/annals.1353.040 |
| format | Article |
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High‐quality immunoassays for these compounds appear to be as accurate as high‐pressure liquid chromatography (HPLC) or gas chromatography/mass spectrometry (GC‐MS). Therefore, the current project aims to establish a new sensitive radioimmunoassay (RIA) for the measurement of free metanephrines in the plasma of patients in the work‐up for pheochromocytoma. We report first results of an ongoing multicenter clinico–chemical evaluation study in hypertensive patients and normotensive volunteers. After an overnight fast plasma samples were collected on ice in EDTA‐ and heparin‐coated tubes after insertion of an indwelling venous line and resting in the supine (patients) or sitting position (normal volunteers) for 30 min. Plasma metanephrines were measured by a newly developed RIA from IBL, Hamburg, Germany. Good agreement of the assay with the tandem mass spectrometry (LC‐MS/MS) method for normetanephrine (r2= 0.975) and for metanephrine (r2= 0.985) could be demonstrated. Both specimens, EDTA and heparin plasma, can be used with the same results. The RIA has a good precision of <15% in the normal range and of <10% in the elevated concentration range. Our preliminary data suggest a high validity of the newly developed RIA for measuring free metanephrine and normetanephrine in hypertensive subjects in both EDTA and heparin plasma. Further work is required to determine the accuracy of the test in larger patient populations and in patients with pheochromocytoma.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1196/annals.1353.040</identifier><identifier>PMID: 17102105</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Adrenal Gland Neoplasms - blood ; Adrenal Gland Neoplasms - diagnosis ; Adult ; Aged ; Aged, 80 and over ; EDTA ; Female ; free plasma metanep- hrines ; heparin ; Humans ; immunoassay ; LC-MS/MS ; Male ; Metanephrine - blood ; Middle Aged ; pheochromocytoma ; Pheochromocytoma - blood ; Pheochromocytoma - diagnosis ; Radioimmunoassay - methods ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>Annals of the New York Academy of Sciences, 2006-08, Vol.1073 (1), p.358-373</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4080-ee514076ec340af869f65b37553a4bf9049755cfb3bbfdbf0b314e3630db3a033</citedby><cites>FETCH-LOGICAL-c4080-ee514076ec340af869f65b37553a4bf9049755cfb3bbfdbf0b314e3630db3a033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1353.040$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1353.040$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17102105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LENZ, TOMAS</creatorcontrib><creatorcontrib>ZORNER, JOACHIM</creatorcontrib><creatorcontrib>KIRCHMAIER, CARL</creatorcontrib><creatorcontrib>PILLITTERI, DANIELE</creatorcontrib><creatorcontrib>BADENHOOP, KLAUS</creatorcontrib><creatorcontrib>BARTEL, CHRISTINE</creatorcontrib><creatorcontrib>GEIGER, HELMUT</creatorcontrib><creatorcontrib>HASSELBACHER, KATRIN</creatorcontrib><creatorcontrib>TUSCHY, ULRICH</creatorcontrib><creatorcontrib>WESTERMANN, JÜRGEN</creatorcontrib><creatorcontrib>SALEWSKI, LOTHAR</creatorcontrib><title>Multicenter Study on the Diagnostic Value of a New RIA for the Detection of Free Plasma Metanephrines in the Work-Up for Pheochromocytoma</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: Available laboratory test methods for the detection of elevated concentrations of catecholamines and their metabolites in urine and/or plasma are not always sensitive enough for the detection of pheochromocytoma. High‐quality immunoassays for these compounds appear to be as accurate as high‐pressure liquid chromatography (HPLC) or gas chromatography/mass spectrometry (GC‐MS). Therefore, the current project aims to establish a new sensitive radioimmunoassay (RIA) for the measurement of free metanephrines in the plasma of patients in the work‐up for pheochromocytoma. We report first results of an ongoing multicenter clinico–chemical evaluation study in hypertensive patients and normotensive volunteers. After an overnight fast plasma samples were collected on ice in EDTA‐ and heparin‐coated tubes after insertion of an indwelling venous line and resting in the supine (patients) or sitting position (normal volunteers) for 30 min. Plasma metanephrines were measured by a newly developed RIA from IBL, Hamburg, Germany. Good agreement of the assay with the tandem mass spectrometry (LC‐MS/MS) method for normetanephrine (r2= 0.975) and for metanephrine (r2= 0.985) could be demonstrated. Both specimens, EDTA and heparin plasma, can be used with the same results. The RIA has a good precision of <15% in the normal range and of <10% in the elevated concentration range. Our preliminary data suggest a high validity of the newly developed RIA for measuring free metanephrine and normetanephrine in hypertensive subjects in both EDTA and heparin plasma. Further work is required to determine the accuracy of the test in larger patient populations and in patients with pheochromocytoma.</description><subject>Adrenal Gland Neoplasms - blood</subject><subject>Adrenal Gland Neoplasms - diagnosis</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>EDTA</subject><subject>Female</subject><subject>free plasma metanep- hrines</subject><subject>heparin</subject><subject>Humans</subject><subject>immunoassay</subject><subject>LC-MS/MS</subject><subject>Male</subject><subject>Metanephrine - blood</subject><subject>Middle Aged</subject><subject>pheochromocytoma</subject><subject>Pheochromocytoma - blood</subject><subject>Pheochromocytoma - diagnosis</subject><subject>Radioimmunoassay - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v0zAYhy0EYt3gzA35xC2dHTtOcqwK-6eumyhjgovluK-pWRIX29HWj8C3xlsqdtzJlt7n98ivfwh9oGRKaS2OVd-rNkwpK9iUcPIKTWjJ60wIlr9GE0LKMqvqnB2gwxB-E0Lzipdv0QEtKckpKSbo7-XQRquhj-DxKg7rHXY9jhvAn6361buQhvi7agfAzmCFl3CPv57PsHF-pCCCjjZl0vjEA-DrVoVO4UuIqoftxtseAraj89b5u-xm-5S-3oDTG-86p3fRdeodemPSLvB-fx6hm5Mv3-Zn2eLq9Hw-W2Sak4pkAAXlpBSgGSfKVKI2omhYWRRM8cbUhNfprk3DmsasG0MaRjkwwci6YYowdoQ-jd6td38GCFF2Nmho2_RcNwQpKsrLPC9eBGnNBRUFTeDxCGrvQvBg5NbbTvmdpEQ-1iTHmuRjTTLVlBIf9-qh6WD9zO97SQAbgXvbwu4ln1z-mK2etNmYsiHCw_-U8ndSlOmL5O3yVBbzxar-ebGSnP0DmAevRA</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>LENZ, TOMAS</creator><creator>ZORNER, JOACHIM</creator><creator>KIRCHMAIER, CARL</creator><creator>PILLITTERI, DANIELE</creator><creator>BADENHOOP, KLAUS</creator><creator>BARTEL, CHRISTINE</creator><creator>GEIGER, HELMUT</creator><creator>HASSELBACHER, KATRIN</creator><creator>TUSCHY, ULRICH</creator><creator>WESTERMANN, JÜRGEN</creator><creator>SALEWSKI, LOTHAR</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200608</creationdate><title>Multicenter Study on the Diagnostic Value of a New RIA for the Detection of Free Plasma Metanephrines in the Work-Up for Pheochromocytoma</title><author>LENZ, TOMAS ; ZORNER, JOACHIM ; KIRCHMAIER, CARL ; PILLITTERI, DANIELE ; BADENHOOP, KLAUS ; BARTEL, CHRISTINE ; GEIGER, HELMUT ; HASSELBACHER, KATRIN ; TUSCHY, ULRICH ; WESTERMANN, JÜRGEN ; SALEWSKI, LOTHAR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4080-ee514076ec340af869f65b37553a4bf9049755cfb3bbfdbf0b314e3630db3a033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adrenal Gland Neoplasms - blood</topic><topic>Adrenal Gland Neoplasms - diagnosis</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>EDTA</topic><topic>Female</topic><topic>free plasma metanep- hrines</topic><topic>heparin</topic><topic>Humans</topic><topic>immunoassay</topic><topic>LC-MS/MS</topic><topic>Male</topic><topic>Metanephrine - blood</topic><topic>Middle Aged</topic><topic>pheochromocytoma</topic><topic>Pheochromocytoma - blood</topic><topic>Pheochromocytoma - diagnosis</topic><topic>Radioimmunoassay - methods</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LENZ, TOMAS</creatorcontrib><creatorcontrib>ZORNER, JOACHIM</creatorcontrib><creatorcontrib>KIRCHMAIER, CARL</creatorcontrib><creatorcontrib>PILLITTERI, DANIELE</creatorcontrib><creatorcontrib>BADENHOOP, KLAUS</creatorcontrib><creatorcontrib>BARTEL, CHRISTINE</creatorcontrib><creatorcontrib>GEIGER, HELMUT</creatorcontrib><creatorcontrib>HASSELBACHER, KATRIN</creatorcontrib><creatorcontrib>TUSCHY, ULRICH</creatorcontrib><creatorcontrib>WESTERMANN, JÜRGEN</creatorcontrib><creatorcontrib>SALEWSKI, LOTHAR</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LENZ, TOMAS</au><au>ZORNER, JOACHIM</au><au>KIRCHMAIER, CARL</au><au>PILLITTERI, DANIELE</au><au>BADENHOOP, KLAUS</au><au>BARTEL, CHRISTINE</au><au>GEIGER, HELMUT</au><au>HASSELBACHER, KATRIN</au><au>TUSCHY, ULRICH</au><au>WESTERMANN, JÜRGEN</au><au>SALEWSKI, LOTHAR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multicenter Study on the Diagnostic Value of a New RIA for the Detection of Free Plasma Metanephrines in the Work-Up for Pheochromocytoma</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2006-08</date><risdate>2006</risdate><volume>1073</volume><issue>1</issue><spage>358</spage><epage>373</epage><pages>358-373</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: Available laboratory test methods for the detection of elevated concentrations of catecholamines and their metabolites in urine and/or plasma are not always sensitive enough for the detection of pheochromocytoma. High‐quality immunoassays for these compounds appear to be as accurate as high‐pressure liquid chromatography (HPLC) or gas chromatography/mass spectrometry (GC‐MS). Therefore, the current project aims to establish a new sensitive radioimmunoassay (RIA) for the measurement of free metanephrines in the plasma of patients in the work‐up for pheochromocytoma. We report first results of an ongoing multicenter clinico–chemical evaluation study in hypertensive patients and normotensive volunteers. After an overnight fast plasma samples were collected on ice in EDTA‐ and heparin‐coated tubes after insertion of an indwelling venous line and resting in the supine (patients) or sitting position (normal volunteers) for 30 min. Plasma metanephrines were measured by a newly developed RIA from IBL, Hamburg, Germany. Good agreement of the assay with the tandem mass spectrometry (LC‐MS/MS) method for normetanephrine (r2= 0.975) and for metanephrine (r2= 0.985) could be demonstrated. Both specimens, EDTA and heparin plasma, can be used with the same results. The RIA has a good precision of <15% in the normal range and of <10% in the elevated concentration range. Our preliminary data suggest a high validity of the newly developed RIA for measuring free metanephrine and normetanephrine in hypertensive subjects in both EDTA and heparin plasma. Further work is required to determine the accuracy of the test in larger patient populations and in patients with pheochromocytoma.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>17102105</pmid><doi>10.1196/annals.1353.040</doi><tpages>16</tpages></addata></record> |
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| ispartof | Annals of the New York Academy of Sciences, 2006-08, Vol.1073 (1), p.358-373 |
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| subjects | Adrenal Gland Neoplasms - blood Adrenal Gland Neoplasms - diagnosis Adult Aged Aged, 80 and over EDTA Female free plasma metanep- hrines heparin Humans immunoassay LC-MS/MS Male Metanephrine - blood Middle Aged pheochromocytoma Pheochromocytoma - blood Pheochromocytoma - diagnosis Radioimmunoassay - methods Reproducibility of Results Sensitivity and Specificity |
| title | Multicenter Study on the Diagnostic Value of a New RIA for the Detection of Free Plasma Metanephrines in the Work-Up for Pheochromocytoma |
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