Label-Free Optical Detection and Tracking of Single Virions Bound to Their Receptors in Supported Membrane Bilayers
We apply an interferometric optical detection scheme to image and track unlabeled single virions. Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound...
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| Veröffentlicht in: | Nano letters 2007-08, Vol.7 (8), p.2263-2266 |
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| container_end_page | 2266 |
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| container_issue | 8 |
| container_start_page | 2263 |
| container_title | Nano letters |
| container_volume | 7 |
| creator | Ewers, Helge Jacobsen, Volker Klotzsch, Enrico Smith, Alicia E Helenius, Ari Sandoghdar, Vahid |
| description | We apply an interferometric optical detection scheme to image and track unlabeled single virions. Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound to supported membrane bilayers via the viral receptor, the glycolipid GM1. The technology presented here promises to be generally applicable to studying the motion of unlabeled macromolecules on membranes. |
| doi_str_mv | 10.1021/nl070766y |
| format | Article |
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Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound to supported membrane bilayers via the viral receptor, the glycolipid GM1. The technology presented here promises to be generally applicable to studying the motion of unlabeled macromolecules on membranes.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl070766y</identifier><identifier>PMID: 17637017</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Binding Sites ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation technics, apparatus ; Lipid Bilayers - chemistry ; Microscopy, Polarization - methods ; Protein Binding ; Receptors, Virus - chemistry ; Receptors, Virus - ultrastructure ; Simian virus 40 ; Staining and Labeling ; Tissues, organs and organisms biophysics ; Virion - ultrastructure</subject><ispartof>Nano letters, 2007-08, Vol.7 (8), p.2263-2266</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a374t-8c773f8d3d74e169a635d32e4b8f5bc8c5919c8bb1776f7d22d2b0bd90f7fcc03</citedby><cites>FETCH-LOGICAL-a374t-8c773f8d3d74e169a635d32e4b8f5bc8c5919c8bb1776f7d22d2b0bd90f7fcc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nl070766y$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nl070766y$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18990773$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17637017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ewers, Helge</creatorcontrib><creatorcontrib>Jacobsen, Volker</creatorcontrib><creatorcontrib>Klotzsch, Enrico</creatorcontrib><creatorcontrib>Smith, Alicia E</creatorcontrib><creatorcontrib>Helenius, Ari</creatorcontrib><creatorcontrib>Sandoghdar, Vahid</creatorcontrib><title>Label-Free Optical Detection and Tracking of Single Virions Bound to Their Receptors in Supported Membrane Bilayers</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>We apply an interferometric optical detection scheme to image and track unlabeled single virions. 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Psychology</topic><topic>General aspects, investigation technics, apparatus</topic><topic>Lipid Bilayers - chemistry</topic><topic>Microscopy, Polarization - methods</topic><topic>Protein Binding</topic><topic>Receptors, Virus - chemistry</topic><topic>Receptors, Virus - ultrastructure</topic><topic>Simian virus 40</topic><topic>Staining and Labeling</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Virion - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ewers, Helge</creatorcontrib><creatorcontrib>Jacobsen, Volker</creatorcontrib><creatorcontrib>Klotzsch, Enrico</creatorcontrib><creatorcontrib>Smith, Alicia E</creatorcontrib><creatorcontrib>Helenius, Ari</creatorcontrib><creatorcontrib>Sandoghdar, Vahid</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ewers, Helge</au><au>Jacobsen, Volker</au><au>Klotzsch, Enrico</au><au>Smith, Alicia E</au><au>Helenius, Ari</au><au>Sandoghdar, Vahid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-Free Optical Detection and Tracking of Single Virions Bound to Their Receptors in Supported Membrane Bilayers</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>7</volume><issue>8</issue><spage>2263</spage><epage>2266</epage><pages>2263-2266</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We apply an interferometric optical detection scheme to image and track unlabeled single virions. 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| fulltext | fulltext |
| identifier | ISSN: 1530-6984 |
| ispartof | Nano letters, 2007-08, Vol.7 (8), p.2263-2266 |
| issn | 1530-6984 1530-6992 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Binding Sites Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Lipid Bilayers - chemistry Microscopy, Polarization - methods Protein Binding Receptors, Virus - chemistry Receptors, Virus - ultrastructure Simian virus 40 Staining and Labeling Tissues, organs and organisms biophysics Virion - ultrastructure |
| title | Label-Free Optical Detection and Tracking of Single Virions Bound to Their Receptors in Supported Membrane Bilayers |
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