Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection
We developed a rapid and sensitive diagnosis system for H5N1 highly pathogenic avian influenza (HPAI) virus infection using an unique gene amplification method, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The sensitivity of the system was found to be 100-fold higher than...
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| Veröffentlicht in: | Vaccine 2006-11, Vol.24 (44), p.6679-6682 |
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| container_issue | 44 |
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| container_title | Vaccine |
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| creator | Imai, Masaki Ninomiya, Ai Minekawa, Harumi Notomi, Tsugunori Ishizaki, Toru Tashiro, Masato Odagiri, Takato |
| description | We developed a rapid and sensitive diagnosis system for H5N1 highly pathogenic avian influenza (HPAI) virus infection using an unique gene amplification method, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The sensitivity of the system was found to be 100-fold higher than that of ordinary one-step RT-PCR. Moreover, by using viral RNAs extracted from influenza viruses of all 15 HA subtypes, the RT-LAMP system was confirmed to amplify only the RNA of H5 subtype virus. In the surveillance of H5N1 virus infection of wild birds, we detected two positive cases from dead crows found near the affected area with H5N1-HPAI by using RT-LAMP system, although one of two positive cases was missed by RT-PCR. These results suggested that our newly developed RT-LAMP system specific for H5 virus would be a beneficial diagnostic tool for surveillance of recent outbreaks caused by H5N1-HPAI viruses. |
| doi_str_mv | 10.1016/j.vaccine.2006.05.046 |
| format | Article |
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The sensitivity of the system was found to be 100-fold higher than that of ordinary one-step RT-PCR. Moreover, by using viral RNAs extracted from influenza viruses of all 15 HA subtypes, the RT-LAMP system was confirmed to amplify only the RNA of H5 subtype virus. In the surveillance of H5N1 virus infection of wild birds, we detected two positive cases from dead crows found near the affected area with H5N1-HPAI by using RT-LAMP system, although one of two positive cases was missed by RT-PCR. These results suggested that our newly developed RT-LAMP system specific for H5 virus would be a beneficial diagnostic tool for surveillance of recent outbreaks caused by H5N1-HPAI viruses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2006.05.046</identifier><identifier>PMID: 16797110</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Applied microbiology ; Avian influenza virus ; Biological and medical sciences ; Chickens ; DNA Primers ; Fundamental and applied biological sciences. Psychology ; Genes ; H5N1 highly pathogenic avian influenza viruses ; Humans ; Infections ; Influenza A Virus, H5N1 Subtype - classification ; Influenza A Virus, H5N1 Subtype - genetics ; Influenza A Virus, H5N1 Subtype - isolation & purification ; Influenza in Birds - diagnosis ; Influenza in Birds - virology ; Laboratories ; Methods ; Microbiology ; Miscellaneous ; Nucleic Acid Amplification Techniques - methods ; Poultry ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - analysis ; RNA, Viral - isolation & purification ; RT-LAMP ; RT-PCR ; Sensitivity and Specificity ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Virology ; Viruses</subject><ispartof>Vaccine, 2006-11, Vol.24 (44), p.6679-6682</ispartof><rights>2006 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Elsevier Limited Nov 10, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-f9f7bf33f43898b113789c5610e73268fbbe1c83187ac9a8fe4bfa49f28d01953</citedby><cites>FETCH-LOGICAL-c551t-f9f7bf33f43898b113789c5610e73268fbbe1c83187ac9a8fe4bfa49f28d01953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1559079231?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18284861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16797110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imai, Masaki</creatorcontrib><creatorcontrib>Ninomiya, Ai</creatorcontrib><creatorcontrib>Minekawa, Harumi</creatorcontrib><creatorcontrib>Notomi, Tsugunori</creatorcontrib><creatorcontrib>Ishizaki, Toru</creatorcontrib><creatorcontrib>Tashiro, Masato</creatorcontrib><creatorcontrib>Odagiri, Takato</creatorcontrib><title>Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>We developed a rapid and sensitive diagnosis system for H5N1 highly pathogenic avian influenza (HPAI) virus infection using an unique gene amplification method, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The sensitivity of the system was found to be 100-fold higher than that of ordinary one-step RT-PCR. Moreover, by using viral RNAs extracted from influenza viruses of all 15 HA subtypes, the RT-LAMP system was confirmed to amplify only the RNA of H5 subtype virus. In the surveillance of H5N1 virus infection of wild birds, we detected two positive cases from dead crows found near the affected area with H5N1-HPAI by using RT-LAMP system, although one of two positive cases was missed by RT-PCR. These results suggested that our newly developed RT-LAMP system specific for H5 virus would be a beneficial diagnostic tool for surveillance of recent outbreaks caused by H5N1-HPAI viruses.</description><subject>Animals</subject><subject>Applied microbiology</subject><subject>Avian influenza virus</subject><subject>Biological and medical sciences</subject><subject>Chickens</subject><subject>DNA Primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>H5N1 highly pathogenic avian influenza viruses</subject><subject>Humans</subject><subject>Infections</subject><subject>Influenza A Virus, H5N1 Subtype - classification</subject><subject>Influenza A Virus, H5N1 Subtype - genetics</subject><subject>Influenza A Virus, H5N1 Subtype - isolation & purification</subject><subject>Influenza in Birds - diagnosis</subject><subject>Influenza in Birds - virology</subject><subject>Laboratories</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Poultry</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - isolation & purification</subject><subject>RT-LAMP</subject><subject>RT-PCR</subject><subject>Sensitivity and Specificity</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Virology</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkkGL1DAYhoso7rj6E5SAKOuhY76maZOTLOvqCiOKrOAtpJkvmqFNatIOrFf_-KbMwIIH9xQCz_vmS54UxXOga6DQvN2t99oY53FdUdqsKV_TunlQrEC0rKw4iIfFilZNXdZAf5wUT1LaUUo5A_m4OIGmlS0AXRV_3-Me-zAO6CcSLLni5bfrcnP--Ss560MYywG3Tk-4JS6F6RfGQfdED2PvrDN6csG_IekmTTgQGyKJenRbkhM_fUguHRqJ3jvtifO2n9H_0WTv4pyWPZql4WnxyOo-4bPjelp8_3B5fXFVbr58_HRxvikN5zCVVtq2s4zZmgkpOgDWCml4AxRbVjXCdh2CESy_gDZSC4t1Z3UtbSW2FCRnp8XrQ-8Yw-8Z06QGlwz2vfYY5qQaATVwWd0LgmSStZJl8Oz_IKe0FaJmC_ryH3QX5ujzfTPFJW3zuZApfqBMDClFtGqMbtDxRgFVi3e1U0fvavGuKFfZe869OLbPXTZ2lzqKzsCrI6CT0b2N2huX7jhRiVo0ywDvDhxmEXuHUSXj0Jv8C2K2pbbB3TPKLezpzXo</recordid><startdate>20061110</startdate><enddate>20061110</enddate><creator>Imai, Masaki</creator><creator>Ninomiya, Ai</creator><creator>Minekawa, Harumi</creator><creator>Notomi, Tsugunori</creator><creator>Ishizaki, Toru</creator><creator>Tashiro, Masato</creator><creator>Odagiri, Takato</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7U2</scope><scope>7X8</scope></search><sort><creationdate>20061110</creationdate><title>Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection</title><author>Imai, Masaki ; Ninomiya, Ai ; Minekawa, Harumi ; Notomi, Tsugunori ; Ishizaki, Toru ; Tashiro, Masato ; Odagiri, Takato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-f9f7bf33f43898b113789c5610e73268fbbe1c83187ac9a8fe4bfa49f28d01953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Applied microbiology</topic><topic>Avian influenza virus</topic><topic>Biological and medical sciences</topic><topic>Chickens</topic><topic>DNA Primers</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imai, Masaki</au><au>Ninomiya, Ai</au><au>Minekawa, Harumi</au><au>Notomi, Tsugunori</au><au>Ishizaki, Toru</au><au>Tashiro, Masato</au><au>Odagiri, Takato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-11-10</date><risdate>2006</risdate><volume>24</volume><issue>44</issue><spage>6679</spage><epage>6682</epage><pages>6679-6682</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>We developed a rapid and sensitive diagnosis system for H5N1 highly pathogenic avian influenza (HPAI) virus infection using an unique gene amplification method, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). The sensitivity of the system was found to be 100-fold higher than that of ordinary one-step RT-PCR. Moreover, by using viral RNAs extracted from influenza viruses of all 15 HA subtypes, the RT-LAMP system was confirmed to amplify only the RNA of H5 subtype virus. In the surveillance of H5N1 virus infection of wild birds, we detected two positive cases from dead crows found near the affected area with H5N1-HPAI by using RT-LAMP system, although one of two positive cases was missed by RT-PCR. These results suggested that our newly developed RT-LAMP system specific for H5 virus would be a beneficial diagnostic tool for surveillance of recent outbreaks caused by H5N1-HPAI viruses.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16797110</pmid><doi>10.1016/j.vaccine.2006.05.046</doi><tpages>4</tpages></addata></record> |
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| ispartof | Vaccine, 2006-11, Vol.24 (44), p.6679-6682 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Animals Applied microbiology Avian influenza virus Biological and medical sciences Chickens DNA Primers Fundamental and applied biological sciences. Psychology Genes H5N1 highly pathogenic avian influenza viruses Humans Infections Influenza A Virus, H5N1 Subtype - classification Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - isolation & purification Influenza in Birds - diagnosis Influenza in Birds - virology Laboratories Methods Microbiology Miscellaneous Nucleic Acid Amplification Techniques - methods Poultry Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis RNA, Viral - isolation & purification RT-LAMP RT-PCR Sensitivity and Specificity Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology Viruses |
| title | Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection |
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