Essential Requirement of Toll-like Receptor 4 Expression on CD11c+ Cells for Locoregional Immunotherapy of Malignant Ascites Using a Streptococcal Preparation OK-432
Toll-like receptors (TLRs) are important molecules that stimulate the innate immunity in order to eradicate microbial pathogens, after which the adaptive immunity emerges. The involvement of TLRs in the action mechanism of OK-432, a bacterial preparation, was investigated in the locoregional treatme...
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| Veröffentlicht in: | Anticancer research 2006-09, Vol.26 (5B), p.3701-3707 |
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| creator | HIRONAKA, Katsuji YAMAGUCHI, Yoshiyuki OKITA, Riki OKAWAKI, Makoto NAGAMINE, Ichiro |
| description | Toll-like receptors (TLRs) are important molecules that stimulate the innate immunity in order to eradicate microbial pathogens,
after which the adaptive immunity emerges. The involvement of TLRs in the action mechanism of OK-432, a bacterial preparation,
was investigated in the locoregional treatment of malignant ascites from gastric cancer. The expression of TLRs in ascites
cells was analyzed using reverse-transcription polymerase chain reaction specific for TLRs and by flow cytometry using anti-TLR2,
-TLR4, -CD4, -CD8, and -CD11c antibodies. These measurements were compared with the locoregional response of OK-432 immunotherapy
for malignant ascites, as well as TNF-α producing potential, which was measured by ELISA, of ascites cells stimulated in vitro
with OK-432. It was observed that OK-432 immunotherapy for malignant ascites showed 8 positive (67%) and 4 negative responses
with the tolerable adverse effects of fever elevation and abdominal pain. The TNF-α production of ascites cells by in vitro
OK-432 stimulation was significantly higher in responder patients than in non-responders. The clinical responses were correlated
with the expression of the TLR4 gene of ascites cells. The TNF-α-producing potential of ascites cells by in vitro OK-432 stimulation
was dependent on the existence of a CD11c+TLR-4+ cell population in ascites cells. OK-432 was highly stimulatory for TNF-α
production of ascites cells compared with other biological response modifiers of PSK and LEM. These results suggest that TLR-4
expression on ascites cells of a macrophage lineage is essential for ascites cells to produce TNF-α in relation to OK-432
stimulation and for subsequent positive clinical responses in locoregional immunotherapy using OK-432 for malignant ascites
from gastric cancer. |
| format | Article |
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after which the adaptive immunity emerges. The involvement of TLRs in the action mechanism of OK-432, a bacterial preparation,
was investigated in the locoregional treatment of malignant ascites from gastric cancer. The expression of TLRs in ascites
cells was analyzed using reverse-transcription polymerase chain reaction specific for TLRs and by flow cytometry using anti-TLR2,
-TLR4, -CD4, -CD8, and -CD11c antibodies. These measurements were compared with the locoregional response of OK-432 immunotherapy
for malignant ascites, as well as TNF-α producing potential, which was measured by ELISA, of ascites cells stimulated in vitro
with OK-432. It was observed that OK-432 immunotherapy for malignant ascites showed 8 positive (67%) and 4 negative responses
with the tolerable adverse effects of fever elevation and abdominal pain. The TNF-α production of ascites cells by in vitro
OK-432 stimulation was significantly higher in responder patients than in non-responders. The clinical responses were correlated
with the expression of the TLR4 gene of ascites cells. The TNF-α-producing potential of ascites cells by in vitro OK-432 stimulation
was dependent on the existence of a CD11c+TLR-4+ cell population in ascites cells. OK-432 was highly stimulatory for TNF-α
production of ascites cells compared with other biological response modifiers of PSK and LEM. These results suggest that TLR-4
expression on ascites cells of a macrophage lineage is essential for ascites cells to produce TNF-α in relation to OK-432
stimulation and for subsequent positive clinical responses in locoregional immunotherapy using OK-432 for malignant ascites
from gastric cancer.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 17094388</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Ascites - metabolism ; Ascites - therapy ; Base Sequence ; Biological and medical sciences ; CD11c Antigen - metabolism ; DNA Primers ; Flow Cytometry ; Humans ; Immunotherapy ; Medical sciences ; Picibanil - therapeutic use ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - therapy ; Toll-Like Receptor 4 - genetics ; Toll-Like Receptor 4 - metabolism ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumors</subject><ispartof>Anticancer research, 2006-09, Vol.26 (5B), p.3701-3707</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18245400$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17094388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HIRONAKA, Katsuji</creatorcontrib><creatorcontrib>YAMAGUCHI, Yoshiyuki</creatorcontrib><creatorcontrib>OKITA, Riki</creatorcontrib><creatorcontrib>OKAWAKI, Makoto</creatorcontrib><creatorcontrib>NAGAMINE, Ichiro</creatorcontrib><title>Essential Requirement of Toll-like Receptor 4 Expression on CD11c+ Cells for Locoregional Immunotherapy of Malignant Ascites Using a Streptococcal Preparation OK-432</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Toll-like receptors (TLRs) are important molecules that stimulate the innate immunity in order to eradicate microbial pathogens,
after which the adaptive immunity emerges. The involvement of TLRs in the action mechanism of OK-432, a bacterial preparation,
was investigated in the locoregional treatment of malignant ascites from gastric cancer. The expression of TLRs in ascites
cells was analyzed using reverse-transcription polymerase chain reaction specific for TLRs and by flow cytometry using anti-TLR2,
-TLR4, -CD4, -CD8, and -CD11c antibodies. These measurements were compared with the locoregional response of OK-432 immunotherapy
for malignant ascites, as well as TNF-α producing potential, which was measured by ELISA, of ascites cells stimulated in vitro
with OK-432. It was observed that OK-432 immunotherapy for malignant ascites showed 8 positive (67%) and 4 negative responses
with the tolerable adverse effects of fever elevation and abdominal pain. The TNF-α production of ascites cells by in vitro
OK-432 stimulation was significantly higher in responder patients than in non-responders. The clinical responses were correlated
with the expression of the TLR4 gene of ascites cells. The TNF-α-producing potential of ascites cells by in vitro OK-432 stimulation
was dependent on the existence of a CD11c+TLR-4+ cell population in ascites cells. OK-432 was highly stimulatory for TNF-α
production of ascites cells compared with other biological response modifiers of PSK and LEM. These results suggest that TLR-4
expression on ascites cells of a macrophage lineage is essential for ascites cells to produce TNF-α in relation to OK-432
stimulation and for subsequent positive clinical responses in locoregional immunotherapy using OK-432 for malignant ascites
from gastric cancer.</description><subject>Ascites - metabolism</subject><subject>Ascites - therapy</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CD11c Antigen - metabolism</subject><subject>DNA Primers</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Medical sciences</subject><subject>Picibanil - therapeutic use</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - therapy</subject><subject>Toll-Like Receptor 4 - genetics</subject><subject>Toll-Like Receptor 4 - metabolism</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkN1u1EAMhSMEotvCK6C5gRsUaX4zyWVZtlCxqAja62gy6-wOTDLpOBH0gXjPesWiSpYty5_Pkf2sWAnbiNIaxZ8XKy4NLy3n5qw4R_zJeVU1tXpZnAnLG63qelX83SDCOAcX2Xe4X0KGgVqWenabYixj-AU08DDNKTPNNn-mDIghjYxi_VEI_56tIUZkPQHb5FOGPY1J73oYljHNB8huejgqfnUx7EdH8pfowwzI7jCMe-bYjzkfHXzynha_UeOym48uN19KreSr4kXvIsLrU70o7q42t-vP5fbm0_X6clsepOVzacHpHkB3ja5604NVvhNGdYLbprLcSMrQWcEbo420VklhtVBa73a6k-DVRfHun-6U0_0COLdDQE_nuRHSgm1VCyVoh8A3J3DpBti1Uw6Dyw_t_8cS8PYEOKSb-uxGH_CJq6U2mvMnx0PYH37T-1scXIwkq1qXZdWaD62yXKhHo9iPvw</recordid><startdate>20060901</startdate><enddate>20060901</enddate><creator>HIRONAKA, Katsuji</creator><creator>YAMAGUCHI, Yoshiyuki</creator><creator>OKITA, Riki</creator><creator>OKAWAKI, Makoto</creator><creator>NAGAMINE, Ichiro</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20060901</creationdate><title>Essential Requirement of Toll-like Receptor 4 Expression on CD11c+ Cells for Locoregional Immunotherapy of Malignant Ascites Using a Streptococcal Preparation OK-432</title><author>HIRONAKA, Katsuji ; YAMAGUCHI, Yoshiyuki ; OKITA, Riki ; OKAWAKI, Makoto ; NAGAMINE, Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h270t-7ea4fee4b946f5fe73cb153b107967052967eb7109545277321741344dd4b2ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Ascites - metabolism</topic><topic>Ascites - therapy</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CD11c Antigen - metabolism</topic><topic>DNA Primers</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunotherapy</topic><topic>Medical sciences</topic><topic>Picibanil - therapeutic use</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - therapy</topic><topic>Toll-Like Receptor 4 - genetics</topic><topic>Toll-Like Receptor 4 - metabolism</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HIRONAKA, Katsuji</creatorcontrib><creatorcontrib>YAMAGUCHI, Yoshiyuki</creatorcontrib><creatorcontrib>OKITA, Riki</creatorcontrib><creatorcontrib>OKAWAKI, Makoto</creatorcontrib><creatorcontrib>NAGAMINE, Ichiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIRONAKA, Katsuji</au><au>YAMAGUCHI, Yoshiyuki</au><au>OKITA, Riki</au><au>OKAWAKI, Makoto</au><au>NAGAMINE, Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Essential Requirement of Toll-like Receptor 4 Expression on CD11c+ Cells for Locoregional Immunotherapy of Malignant Ascites Using a Streptococcal Preparation OK-432</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2006-09-01</date><risdate>2006</risdate><volume>26</volume><issue>5B</issue><spage>3701</spage><epage>3707</epage><pages>3701-3707</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Toll-like receptors (TLRs) are important molecules that stimulate the innate immunity in order to eradicate microbial pathogens,
after which the adaptive immunity emerges. The involvement of TLRs in the action mechanism of OK-432, a bacterial preparation,
was investigated in the locoregional treatment of malignant ascites from gastric cancer. The expression of TLRs in ascites
cells was analyzed using reverse-transcription polymerase chain reaction specific for TLRs and by flow cytometry using anti-TLR2,
-TLR4, -CD4, -CD8, and -CD11c antibodies. These measurements were compared with the locoregional response of OK-432 immunotherapy
for malignant ascites, as well as TNF-α producing potential, which was measured by ELISA, of ascites cells stimulated in vitro
with OK-432. It was observed that OK-432 immunotherapy for malignant ascites showed 8 positive (67%) and 4 negative responses
with the tolerable adverse effects of fever elevation and abdominal pain. The TNF-α production of ascites cells by in vitro
OK-432 stimulation was significantly higher in responder patients than in non-responders. The clinical responses were correlated
with the expression of the TLR4 gene of ascites cells. The TNF-α-producing potential of ascites cells by in vitro OK-432 stimulation
was dependent on the existence of a CD11c+TLR-4+ cell population in ascites cells. OK-432 was highly stimulatory for TNF-α
production of ascites cells compared with other biological response modifiers of PSK and LEM. These results suggest that TLR-4
expression on ascites cells of a macrophage lineage is essential for ascites cells to produce TNF-α in relation to OK-432
stimulation and for subsequent positive clinical responses in locoregional immunotherapy using OK-432 for malignant ascites
from gastric cancer.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>17094388</pmid><tpages>7</tpages></addata></record> |
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| subjects | Ascites - metabolism Ascites - therapy Base Sequence Biological and medical sciences CD11c Antigen - metabolism DNA Primers Flow Cytometry Humans Immunotherapy Medical sciences Picibanil - therapeutic use Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - therapy Toll-Like Receptor 4 - genetics Toll-Like Receptor 4 - metabolism Tumor Necrosis Factor-alpha - biosynthesis Tumors |
| title | Essential Requirement of Toll-like Receptor 4 Expression on CD11c+ Cells for Locoregional Immunotherapy of Malignant Ascites Using a Streptococcal Preparation OK-432 |
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