Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma
Abstract In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1 ....
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| Veröffentlicht in: | Cancer letters 2007-09, Vol.254 (2), p.298-307 |
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| creator | Scintu, Marina Vitale, Rita Prencipe, Maria Pia Gallo, Antonietta Bonghi, Loriana Valori, Vanna Maria Maiello, Evaristo Rinaldi, Monica Signori, Emanuela Rabitti, Carla Carella, Massimo Dallapiccola, Bruno Altomare, Vittorio Fazio, Vito M Parrella, Paola |
| description | Abstract In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1 . All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24–76.78%) than in controls (average: 11.54%, range: 9.91–14.84%) ( P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34–35.8%) as compared with controls (average: 4.18% range: 3.47–4.81%) ( P < 0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability ( r = 0.722, P = 0.018). |
| doi_str_mv | 10.1016/j.canlet.2007.03.021 |
| format | Article |
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All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24–76.78%) than in controls (average: 11.54%, range: 9.91–14.84%) ( P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34–35.8%) as compared with controls (average: 4.18% range: 3.47–4.81%) ( P < 0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability ( r = 0.722, P = 0.018).</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2007.03.021</identifier><identifier>PMID: 17498870</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adult ; Aged ; Breast cancer ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Breast Neoplasms - surgery ; BUB1B ; Calcium-Binding Proteins - genetics ; Carcinoma, Ductal - genetics ; Carcinoma, Ductal - pathology ; Carcinoma, Ductal - surgery ; Cell Cycle Proteins - genetics ; Chromosomal Instability ; Chromosomes ; Chromosomes, Human, Pair 13 ; Chromosomes, Human, Pair 17 ; Chromosomes, Human, Pair 8 ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genes ; Genomic Instability ; Hematology, Oncology and Palliative Medicine ; Humans ; In Situ Hybridization, Fluorescence ; Mad2 Proteins ; MAD2L1 ; Middle Aged ; Mutation ; Protein-Serine-Threonine Kinases - genetics ; Repressor Proteins - genetics ; RNA, Messenger - genetics</subject><ispartof>Cancer letters, 2007-09, Vol.254 (2), p.298-307</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2007 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Sep 8, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-afdf5a9f5d1870ae9712ac3dd8d5c97717bec7504e6e8f8938873279e17481a83</citedby><cites>FETCH-LOGICAL-c540t-afdf5a9f5d1870ae9712ac3dd8d5c97717bec7504e6e8f8938873279e17481a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.canlet.2007.03.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17498870$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scintu, Marina</creatorcontrib><creatorcontrib>Vitale, Rita</creatorcontrib><creatorcontrib>Prencipe, Maria</creatorcontrib><creatorcontrib>Pia Gallo, Antonietta</creatorcontrib><creatorcontrib>Bonghi, Loriana</creatorcontrib><creatorcontrib>Valori, Vanna Maria</creatorcontrib><creatorcontrib>Maiello, Evaristo</creatorcontrib><creatorcontrib>Rinaldi, Monica</creatorcontrib><creatorcontrib>Signori, Emanuela</creatorcontrib><creatorcontrib>Rabitti, Carla</creatorcontrib><creatorcontrib>Carella, Massimo</creatorcontrib><creatorcontrib>Dallapiccola, Bruno</creatorcontrib><creatorcontrib>Altomare, Vittorio</creatorcontrib><creatorcontrib>Fazio, Vito M</creatorcontrib><creatorcontrib>Parrella, Paola</creatorcontrib><title>Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1 . All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24–76.78%) than in controls (average: 11.54%, range: 9.91–14.84%) ( P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34–35.8%) as compared with controls (average: 4.18% range: 3.47–4.81%) ( P < 0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability ( r = 0.722, P = 0.018).</description><subject>Adult</subject><subject>Aged</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - surgery</subject><subject>BUB1B</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Carcinoma, Ductal - genetics</subject><subject>Carcinoma, Ductal - pathology</subject><subject>Carcinoma, Ductal - surgery</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Chromosomal Instability</subject><subject>Chromosomes</subject><subject>Chromosomes, Human, Pair 13</subject><subject>Chromosomes, Human, Pair 17</subject><subject>Chromosomes, Human, Pair 8</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Genomic Instability</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Mad2 Proteins</subject><subject>MAD2L1</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>RNA, Messenger - genetics</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFklGL1DAUhYMo7rj6D0QCgm-tN0nTpC_CzqqrMOKD7nPIJLeSsZOOSbs4_97UGVjYl30Kge-c5NxzCXnNoGbA2ve72tk44FRzAFWDqIGzJ2TFtOKV6jQ8JSsQ0FRCC3lBXuS8AwDZKPmcXDDVdForWBF7g3HcB0dDzJPdhiFMR2qjL3eX0Gb0FP8eEuYcxkjHnq5v12z9n_h29ZFvGP2FEXPBqZ_dZAe6XWQTdTa5UKztS_Kst0PGV-fzktx-_vTz-ku1-X7z9fpqUznZwFTZ3vfSdr30JQFY7BTj1gnvtZeuU4qpLTolocEWda87Uf4vuOqwZNHManFJ3p18D2n8M2OezD5kh8NgI45zNq1mAphoHwU5g4ZxvoBvH4C7cU6xhDBMllG2HROiUM2JcmnMOWFvDinsbToaBmZpyuzMqSmzNGVAmNJUkb05m8_bPfp70bmaAnw4AViGdhcwmewCRoc-JHST8WN47IWHBm4IMTg7_MYj5vssJnMD5seyLcuygAJgkmvxD5BjuQc</recordid><startdate>20070908</startdate><enddate>20070908</enddate><creator>Scintu, Marina</creator><creator>Vitale, Rita</creator><creator>Prencipe, Maria</creator><creator>Pia Gallo, Antonietta</creator><creator>Bonghi, Loriana</creator><creator>Valori, Vanna Maria</creator><creator>Maiello, Evaristo</creator><creator>Rinaldi, Monica</creator><creator>Signori, Emanuela</creator><creator>Rabitti, Carla</creator><creator>Carella, Massimo</creator><creator>Dallapiccola, Bruno</creator><creator>Altomare, Vittorio</creator><creator>Fazio, Vito M</creator><creator>Parrella, Paola</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070908</creationdate><title>Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma</title><author>Scintu, Marina ; 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All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24–76.78%) than in controls (average: 11.54%, range: 9.91–14.84%) ( P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34–35.8%) as compared with controls (average: 4.18% range: 3.47–4.81%) ( P < 0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability ( r = 0.722, P = 0.018).</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>17498870</pmid><doi>10.1016/j.canlet.2007.03.021</doi><tpages>10</tpages></addata></record> |
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| subjects | Adult Aged Breast cancer Breast Neoplasms - genetics Breast Neoplasms - pathology Breast Neoplasms - surgery BUB1B Calcium-Binding Proteins - genetics Carcinoma, Ductal - genetics Carcinoma, Ductal - pathology Carcinoma, Ductal - surgery Cell Cycle Proteins - genetics Chromosomal Instability Chromosomes Chromosomes, Human, Pair 13 Chromosomes, Human, Pair 17 Chromosomes, Human, Pair 8 Female Gene expression Gene Expression Regulation, Neoplastic Genes Genomic Instability Hematology, Oncology and Palliative Medicine Humans In Situ Hybridization, Fluorescence Mad2 Proteins MAD2L1 Middle Aged Mutation Protein-Serine-Threonine Kinases - genetics Repressor Proteins - genetics RNA, Messenger - genetics |
| title | Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma |
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