Chaplet Column Chromatography: Isolation of a Large Set of Individual RNAs in a Single Step
RNA molecules mature through various posttranscriptional modifications and editing. To characterize base modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. Howe...
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| Veröffentlicht in: | Methods in Enzymology 2007, Vol.425, p.231-239 |
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| container_title | Methods in Enzymology |
| container_volume | 425 |
| creator | Suzuki, Takeo Suzuki, Tsutomu |
| description | RNA molecules mature through various posttranscriptional modifications and editing. To characterize base modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. However, there have been no general and convenient strategies for isolation of individual RNAs. We describe a simple and practical method for effective isolation of multiple individual RNA species named “Chaplet Column Chromatography (CCC).” We successfully isolated all species of mammalian mitochondrial tRNAs with this method. |
| doi_str_mv | 10.1016/S0076-6879(07)25010-4 |
| format | Article |
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To characterize base modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. However, there have been no general and convenient strategies for isolation of individual RNAs. 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| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 2007, Vol.425, p.231-239 |
| issn | 0076-6879 1557-7988 |
| language | eng |
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| source | MEDLINE; ScienceDirect eBooks; Access via ScienceDirect (Elsevier) |
| subjects | Animals Cattle Chromatography, Affinity - methods Molecular Probe Techniques RNA - isolation & purification |
| title | Chaplet Column Chromatography: Isolation of a Large Set of Individual RNAs in a Single Step |
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