Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile
Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 ge...
Gespeichert in:
| Veröffentlicht in: | International journal of oncology 2006-12, Vol.29 (6), p.1445-1451 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1451 |
|---|---|
| container_issue | 6 |
| container_start_page | 1445 |
| container_title | International journal of oncology |
| container_volume | 29 |
| creator | TSUNEDOMI, Ryouichi IIZUKA, Norio HAMAMOTO, Yoshihiko YAMADA, Mamoru OKA, Masaaki YAMADA-OKABE, Hisafumi TAMESA, Takao OKADA, Toshimasa SAKAMOTO, Kazuhiko TAKASHIMA, Motonari HAMAGUCHI, Takeshi MIYAMOTO, Takanobu UCHIMURA, Shunji |
| description | Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 genes in 35 moderately differentiated HCV-related HCCs by DNA microarray. Our supervised learning method identified 35 genes involved in PVI. Among the 35 identified genes, we focused on the inhibitor of DNA binding 2 (ID2), because it encodes a liver-rich dominant-negative helix-loop-helix protein. The microarray results for ID2 were reproduced by quantitative real-time reverse transcription (QRT)-PCR and Western blot analyses. In an independent set of HCV-related HCCs (n=28) and HCV-unrelated HCCs (n=14), our QRT-PCR showed that decrease in ID2 mRNA levels were associated with PVI in HCV-related HCC but not HCV-unrelated HCC. In conclusion, our results strongly suggest that ID2 plays an important role in PVI process of HCV-related HCC. |
| doi_str_mv | 10.3892/ijo.29.6.1445 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68120236</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68120236</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-bdd9b6ca4dc1db683bd5f681a1c5be86890fed276e7f02d14a75c019cb527d0b3</originalsourceid><addsrcrecordid>eNqF0TtvFDEUBWALgUhIKGmRG9LN4td47BItAVaKRENqy49r4mh2vNgzgVT8dbybkVJS2dL9dOyrg9A7SjZcafYx3ecN0xu5oUL0L9A5HTTtmGD8ZbsTqjspuD5Db2q9J4T1PaGv0RkdiFJa8XP0dxdgmlNM3s4pTzhHvPvMsK01-2RnCPh3mu9wmh5sXed3cGh2ThVv8UMqS-0KjCd6mmQP47iMtmBvi09T3lvsHvFPmADDn0OBego6lBzTCJfoVbRjhbfreYFuv1z_2H7rbr5_3W0_3XSeazp3LgTtpLcieBqcVNyFPkpFLfW9AyWVJhECGyQMkbBAhR1637b3rmdDII5foKun3PburwXqbPapHn9qJ8hLNS2LEcblfyHVSgxC0wa7J-hLrrVANIeS9rY8GkrMsRrTqjFMG2mO1TT_fg1e3B7Cs167aODDCmz1dozFTj7VZ6c4kYIp_g9xL5ok</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19847491</pqid></control><display><type>article</type><title>Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>TSUNEDOMI, Ryouichi ; IIZUKA, Norio ; HAMAMOTO, Yoshihiko ; YAMADA, Mamoru ; OKA, Masaaki ; YAMADA-OKABE, Hisafumi ; TAMESA, Takao ; OKADA, Toshimasa ; SAKAMOTO, Kazuhiko ; TAKASHIMA, Motonari ; HAMAGUCHI, Takeshi ; MIYAMOTO, Takanobu ; UCHIMURA, Shunji</creator><creatorcontrib>TSUNEDOMI, Ryouichi ; IIZUKA, Norio ; HAMAMOTO, Yoshihiko ; YAMADA, Mamoru ; OKA, Masaaki ; YAMADA-OKABE, Hisafumi ; TAMESA, Takao ; OKADA, Toshimasa ; SAKAMOTO, Kazuhiko ; TAKASHIMA, Motonari ; HAMAGUCHI, Takeshi ; MIYAMOTO, Takanobu ; UCHIMURA, Shunji</creatorcontrib><description>Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 genes in 35 moderately differentiated HCV-related HCCs by DNA microarray. Our supervised learning method identified 35 genes involved in PVI. Among the 35 identified genes, we focused on the inhibitor of DNA binding 2 (ID2), because it encodes a liver-rich dominant-negative helix-loop-helix protein. The microarray results for ID2 were reproduced by quantitative real-time reverse transcription (QRT)-PCR and Western blot analyses. In an independent set of HCV-related HCCs (n=28) and HCV-unrelated HCCs (n=14), our QRT-PCR showed that decrease in ID2 mRNA levels were associated with PVI in HCV-related HCC but not HCV-unrelated HCC. In conclusion, our results strongly suggest that ID2 plays an important role in PVI process of HCV-related HCC.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.29.6.1445</identifier><identifier>PMID: 17088983</identifier><language>eng</language><publisher>Athens: Editorial Academy of the International Journal of Oncology</publisher><subject>Biological and medical sciences ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - virology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Profiling ; Hepatitis C - complications ; Hepatitis C - genetics ; Hepatitis C virus ; Humans ; Inhibitor of Differentiation Protein 2 - biosynthesis ; Inhibitor of Differentiation Protein 2 - genetics ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - virology ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Middle Aged ; Neoplasm Metastasis ; Oligonucleotide Array Sequence Analysis - methods ; Tumors</subject><ispartof>International journal of oncology, 2006-12, Vol.29 (6), p.1445-1451</ispartof><rights>2007 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-bdd9b6ca4dc1db683bd5f681a1c5be86890fed276e7f02d14a75c019cb527d0b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18306428$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17088983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TSUNEDOMI, Ryouichi</creatorcontrib><creatorcontrib>IIZUKA, Norio</creatorcontrib><creatorcontrib>HAMAMOTO, Yoshihiko</creatorcontrib><creatorcontrib>YAMADA, Mamoru</creatorcontrib><creatorcontrib>OKA, Masaaki</creatorcontrib><creatorcontrib>YAMADA-OKABE, Hisafumi</creatorcontrib><creatorcontrib>TAMESA, Takao</creatorcontrib><creatorcontrib>OKADA, Toshimasa</creatorcontrib><creatorcontrib>SAKAMOTO, Kazuhiko</creatorcontrib><creatorcontrib>TAKASHIMA, Motonari</creatorcontrib><creatorcontrib>HAMAGUCHI, Takeshi</creatorcontrib><creatorcontrib>MIYAMOTO, Takanobu</creatorcontrib><creatorcontrib>UCHIMURA, Shunji</creatorcontrib><title>Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 genes in 35 moderately differentiated HCV-related HCCs by DNA microarray. Our supervised learning method identified 35 genes involved in PVI. Among the 35 identified genes, we focused on the inhibitor of DNA binding 2 (ID2), because it encodes a liver-rich dominant-negative helix-loop-helix protein. The microarray results for ID2 were reproduced by quantitative real-time reverse transcription (QRT)-PCR and Western blot analyses. In an independent set of HCV-related HCCs (n=28) and HCV-unrelated HCCs (n=14), our QRT-PCR showed that decrease in ID2 mRNA levels were associated with PVI in HCV-related HCC but not HCV-unrelated HCC. In conclusion, our results strongly suggest that ID2 plays an important role in PVI process of HCV-related HCC.</description><subject>Biological and medical sciences</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Profiling</subject><subject>Hepatitis C - complications</subject><subject>Hepatitis C - genetics</subject><subject>Hepatitis C virus</subject><subject>Humans</subject><subject>Inhibitor of Differentiation Protein 2 - biosynthesis</subject><subject>Inhibitor of Differentiation Protein 2 - genetics</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - virology</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Metastasis</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Tumors</subject><issn>1019-6439</issn><issn>1791-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0TtvFDEUBWALgUhIKGmRG9LN4td47BItAVaKRENqy49r4mh2vNgzgVT8dbybkVJS2dL9dOyrg9A7SjZcafYx3ecN0xu5oUL0L9A5HTTtmGD8ZbsTqjspuD5Db2q9J4T1PaGv0RkdiFJa8XP0dxdgmlNM3s4pTzhHvPvMsK01-2RnCPh3mu9wmh5sXed3cGh2ThVv8UMqS-0KjCd6mmQP47iMtmBvi09T3lvsHvFPmADDn0OBego6lBzTCJfoVbRjhbfreYFuv1z_2H7rbr5_3W0_3XSeazp3LgTtpLcieBqcVNyFPkpFLfW9AyWVJhECGyQMkbBAhR1637b3rmdDII5foKun3PburwXqbPapHn9qJ8hLNS2LEcblfyHVSgxC0wa7J-hLrrVANIeS9rY8GkrMsRrTqjFMG2mO1TT_fg1e3B7Cs167aODDCmz1dozFTj7VZ6c4kYIp_g9xL5ok</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>TSUNEDOMI, Ryouichi</creator><creator>IIZUKA, Norio</creator><creator>HAMAMOTO, Yoshihiko</creator><creator>YAMADA, Mamoru</creator><creator>OKA, Masaaki</creator><creator>YAMADA-OKABE, Hisafumi</creator><creator>TAMESA, Takao</creator><creator>OKADA, Toshimasa</creator><creator>SAKAMOTO, Kazuhiko</creator><creator>TAKASHIMA, Motonari</creator><creator>HAMAGUCHI, Takeshi</creator><creator>MIYAMOTO, Takanobu</creator><creator>UCHIMURA, Shunji</creator><general>Editorial Academy of the International Journal of Oncology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20061201</creationdate><title>Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile</title><author>TSUNEDOMI, Ryouichi ; IIZUKA, Norio ; HAMAMOTO, Yoshihiko ; YAMADA, Mamoru ; OKA, Masaaki ; YAMADA-OKABE, Hisafumi ; TAMESA, Takao ; OKADA, Toshimasa ; SAKAMOTO, Kazuhiko ; TAKASHIMA, Motonari ; HAMAGUCHI, Takeshi ; MIYAMOTO, Takanobu ; UCHIMURA, Shunji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-bdd9b6ca4dc1db683bd5f681a1c5be86890fed276e7f02d14a75c019cb527d0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - virology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Profiling</topic><topic>Hepatitis C - complications</topic><topic>Hepatitis C - genetics</topic><topic>Hepatitis C virus</topic><topic>Humans</topic><topic>Inhibitor of Differentiation Protein 2 - biosynthesis</topic><topic>Inhibitor of Differentiation Protein 2 - genetics</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - virology</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Metastasis</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>TSUNEDOMI, Ryouichi</creatorcontrib><creatorcontrib>IIZUKA, Norio</creatorcontrib><creatorcontrib>HAMAMOTO, Yoshihiko</creatorcontrib><creatorcontrib>YAMADA, Mamoru</creatorcontrib><creatorcontrib>OKA, Masaaki</creatorcontrib><creatorcontrib>YAMADA-OKABE, Hisafumi</creatorcontrib><creatorcontrib>TAMESA, Takao</creatorcontrib><creatorcontrib>OKADA, Toshimasa</creatorcontrib><creatorcontrib>SAKAMOTO, Kazuhiko</creatorcontrib><creatorcontrib>TAKASHIMA, Motonari</creatorcontrib><creatorcontrib>HAMAGUCHI, Takeshi</creatorcontrib><creatorcontrib>MIYAMOTO, Takanobu</creatorcontrib><creatorcontrib>UCHIMURA, Shunji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TSUNEDOMI, Ryouichi</au><au>IIZUKA, Norio</au><au>HAMAMOTO, Yoshihiko</au><au>YAMADA, Mamoru</au><au>OKA, Masaaki</au><au>YAMADA-OKABE, Hisafumi</au><au>TAMESA, Takao</au><au>OKADA, Toshimasa</au><au>SAKAMOTO, Kazuhiko</au><au>TAKASHIMA, Motonari</au><au>HAMAGUCHI, Takeshi</au><au>MIYAMOTO, Takanobu</au><au>UCHIMURA, Shunji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile</atitle><jtitle>International journal of oncology</jtitle><addtitle>Int J Oncol</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>29</volume><issue>6</issue><spage>1445</spage><epage>1451</epage><pages>1445-1451</pages><issn>1019-6439</issn><eissn>1791-2423</eissn><abstract>Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 genes in 35 moderately differentiated HCV-related HCCs by DNA microarray. Our supervised learning method identified 35 genes involved in PVI. Among the 35 identified genes, we focused on the inhibitor of DNA binding 2 (ID2), because it encodes a liver-rich dominant-negative helix-loop-helix protein. The microarray results for ID2 were reproduced by quantitative real-time reverse transcription (QRT)-PCR and Western blot analyses. In an independent set of HCV-related HCCs (n=28) and HCV-unrelated HCCs (n=14), our QRT-PCR showed that decrease in ID2 mRNA levels were associated with PVI in HCV-related HCC but not HCV-unrelated HCC. In conclusion, our results strongly suggest that ID2 plays an important role in PVI process of HCV-related HCC.</abstract><cop>Athens</cop><pub>Editorial Academy of the International Journal of Oncology</pub><pmid>17088983</pmid><doi>10.3892/ijo.29.6.1445</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1019-6439 |
| ispartof | International journal of oncology, 2006-12, Vol.29 (6), p.1445-1451 |
| issn | 1019-6439 1791-2423 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68120236 |
| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - virology Female Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Profiling Hepatitis C - complications Hepatitis C - genetics Hepatitis C virus Humans Inhibitor of Differentiation Protein 2 - biosynthesis Inhibitor of Differentiation Protein 2 - genetics Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - virology Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Neoplasm Metastasis Oligonucleotide Array Sequence Analysis - methods Tumors |
| title | Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T07%3A42%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20ID2%20associated%20with%20invasion%20of%20hepatitis%20C%20virus-related%20hepatocellular%20carcinoma%20by%20gene%20expression%20profile&rft.jtitle=International%20journal%20of%20oncology&rft.au=TSUNEDOMI,%20Ryouichi&rft.date=2006-12-01&rft.volume=29&rft.issue=6&rft.spage=1445&rft.epage=1451&rft.pages=1445-1451&rft.issn=1019-6439&rft.eissn=1791-2423&rft_id=info:doi/10.3892/ijo.29.6.1445&rft_dat=%3Cproquest_cross%3E68120236%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19847491&rft_id=info:pmid/17088983&rfr_iscdi=true |