Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies
Polyplexes consisting of plasmid DNA and polycations have received much attention as promising vectors for gene transfer. For effective gene therapy, polycations with different polyamine structures in the side chain were developed to ensure their buffering capacity for endosomal escape, and their PE...
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| Veröffentlicht in: | Journal of controlled release 2007-08, Vol.121 (1), p.38-48 |
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| creator | Han, Muri Bae, Younsoo Nishiyama, Nobuhiro Miyata, Kanjiro Oba, Makoto Kataoka, Kazunori |
| description | Polyplexes consisting of plasmid DNA and polycations have received much attention as promising vectors for gene transfer. For effective gene therapy, polycations with different polyamine structures in the side chain were developed to ensure their buffering capacity for endosomal escape, and their PEGylated block copolymers were developed to increase their stability and biocompatibility. The effects of the chemical structures of polycations and their PEGylation on transfection and cytotoxicity were elucidated by use of a three-dimensional multicellular tumor spheroid of human hepatoma HuH-7 cells. Various features of transfection with polyplex micelles, which have been hard to observe in conventional monolayer cultures, were revealed by the multicellular tumor spheroid (MCTS) model in terms of cytotoxicity and time-dependent behaviors of transfected gene expression under three-dimensional microenvironments. By using this system, the polyplex micelle from poly(ethylene glycol)-
b-poly(
N-substituted asparagine) copolymers having the
N-(2-aminoethyl)-2-aminoethyl group in the side chain (PEG-
b-P[Asp(DET)] polyplex micelle) was proved to achieve high transfection efficiencies as well as low cytotoxicity, both of which are critical properties for successful in vivo gene delivery. |
| doi_str_mv | 10.1016/j.jconrel.2007.05.012 |
| format | Article |
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b-poly(
N-substituted asparagine) copolymers having the
N-(2-aminoethyl)-2-aminoethyl group in the side chain (PEG-
b-P[Asp(DET)] polyplex micelle) was proved to achieve high transfection efficiencies as well as low cytotoxicity, both of which are critical properties for successful in vivo gene delivery.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2007.05.012</identifier><identifier>PMID: 17582637</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biocompatible Materials ; Biological and medical sciences ; Block copolymer ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cell Survival ; Gene Expression ; General pharmacology ; Genes, Reporter ; Genetic Vectors ; Humans ; Hydrogen-Ion Concentration ; Liver Neoplasms - pathology ; Luciferases - metabolism ; Luminescent Measurements ; Medical sciences ; Micelles ; Nanotechnology ; Non-viral gene vector ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Plasmids - genetics ; Polyamines - chemistry ; Polyethylene Glycols - chemistry ; Polymers - chemical synthesis ; Polymers - chemistry ; Polyplex micelle ; Spheroid ; Spheroids, Cellular ; Transfection - methods</subject><ispartof>Journal of controlled release, 2007-08, Vol.121 (1), p.38-48</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-d80e419cd2a13e03748eb91894580a44a717213633ec51e6f9139350f14562ad3</citedby><cites>FETCH-LOGICAL-c424t-d80e419cd2a13e03748eb91894580a44a717213633ec51e6f9139350f14562ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jconrel.2007.05.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,777,781,786,787,3537,23911,23912,25121,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19005397$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17582637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Muri</creatorcontrib><creatorcontrib>Bae, Younsoo</creatorcontrib><creatorcontrib>Nishiyama, Nobuhiro</creatorcontrib><creatorcontrib>Miyata, Kanjiro</creatorcontrib><creatorcontrib>Oba, Makoto</creatorcontrib><creatorcontrib>Kataoka, Kazunori</creatorcontrib><title>Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>Polyplexes consisting of plasmid DNA and polycations have received much attention as promising vectors for gene transfer. For effective gene therapy, polycations with different polyamine structures in the side chain were developed to ensure their buffering capacity for endosomal escape, and their PEGylated block copolymers were developed to increase their stability and biocompatibility. The effects of the chemical structures of polycations and their PEGylation on transfection and cytotoxicity were elucidated by use of a three-dimensional multicellular tumor spheroid of human hepatoma HuH-7 cells. Various features of transfection with polyplex micelles, which have been hard to observe in conventional monolayer cultures, were revealed by the multicellular tumor spheroid (MCTS) model in terms of cytotoxicity and time-dependent behaviors of transfected gene expression under three-dimensional microenvironments. By using this system, the polyplex micelle from poly(ethylene glycol)-
b-poly(
N-substituted asparagine) copolymers having the
N-(2-aminoethyl)-2-aminoethyl group in the side chain (PEG-
b-P[Asp(DET)] polyplex micelle) was proved to achieve high transfection efficiencies as well as low cytotoxicity, both of which are critical properties for successful in vivo gene delivery.</description><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>Block copolymer</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Gene Expression</subject><subject>General pharmacology</subject><subject>Genes, Reporter</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Liver Neoplasms - pathology</subject><subject>Luciferases - metabolism</subject><subject>Luminescent Measurements</subject><subject>Medical sciences</subject><subject>Micelles</subject><subject>Nanotechnology</subject><subject>Non-viral gene vector</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids - genetics</subject><subject>Polyamines - chemistry</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polymers - chemical synthesis</subject><subject>Polymers - chemistry</subject><subject>Polyplex micelle</subject><subject>Spheroid</subject><subject>Spheroids, Cellular</subject><subject>Transfection - methods</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURiMEokPhEUDewC6DHf8kXiFUUUCqxKasLde5mfHIsQfbmTYvw7PidCKVXReWZfnc6-vvVNV7grcEE_H5sD2Y4CO4bYNxu8V8i0nzotqQrqU1k5K_rDaF62oquLyo3qR0wBhzytrX1QVpedcI2m6qv7dR-zSAyTZ4lPLUz2hK1u_QOLlsDTg3OR1RnsYQUTruIQbbJzQsJxMB_ML64OuTjdqhY3DzCNEatAMP6FQah5jQvc175MI9MnMOOTxYY_OMtO_R3u72KP8_BAxDuQZfVnpbvRq0S_Bu3S-r39ffbq9-1De_vv-8-npTG9awXPcdBkak6RtNKGDasg7uJOkk4x3WjOmWtA2hglIwnIAYJKGScjwQxkWje3pZfTr3PcbwZ4KU1WjT8nntIUxJiY4QiZl4FiRSEIFbVkB-Bk0MKUUY1DHaUcdZEawWg-qgVoNqMagwV8VgqfuwPjDdjdA_Va3KCvBxBXQy2g0lOmPTEycXy3Lhvpw5KLmdLESVHlOF3saStOqDfWaUf3jwwGk</recordid><startdate>20070816</startdate><enddate>20070816</enddate><creator>Han, Muri</creator><creator>Bae, Younsoo</creator><creator>Nishiyama, Nobuhiro</creator><creator>Miyata, Kanjiro</creator><creator>Oba, Makoto</creator><creator>Kataoka, Kazunori</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070816</creationdate><title>Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies</title><author>Han, Muri ; Bae, Younsoo ; Nishiyama, Nobuhiro ; Miyata, Kanjiro ; Oba, Makoto ; Kataoka, Kazunori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-d80e419cd2a13e03748eb91894580a44a717213633ec51e6f9139350f14562ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biocompatible Materials</topic><topic>Biological and medical sciences</topic><topic>Block copolymer</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Gene Expression</topic><topic>General pharmacology</topic><topic>Genes, Reporter</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Liver Neoplasms - pathology</topic><topic>Luciferases - metabolism</topic><topic>Luminescent Measurements</topic><topic>Medical sciences</topic><topic>Micelles</topic><topic>Nanotechnology</topic><topic>Non-viral gene vector</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids - genetics</topic><topic>Polyamines - chemistry</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polymers - chemical synthesis</topic><topic>Polymers - chemistry</topic><topic>Polyplex micelle</topic><topic>Spheroid</topic><topic>Spheroids, Cellular</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Muri</creatorcontrib><creatorcontrib>Bae, Younsoo</creatorcontrib><creatorcontrib>Nishiyama, Nobuhiro</creatorcontrib><creatorcontrib>Miyata, Kanjiro</creatorcontrib><creatorcontrib>Oba, Makoto</creatorcontrib><creatorcontrib>Kataoka, Kazunori</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Muri</au><au>Bae, Younsoo</au><au>Nishiyama, Nobuhiro</au><au>Miyata, Kanjiro</au><au>Oba, Makoto</au><au>Kataoka, Kazunori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2007-08-16</date><risdate>2007</risdate><volume>121</volume><issue>1</issue><spage>38</spage><epage>48</epage><pages>38-48</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>Polyplexes consisting of plasmid DNA and polycations have received much attention as promising vectors for gene transfer. For effective gene therapy, polycations with different polyamine structures in the side chain were developed to ensure their buffering capacity for endosomal escape, and their PEGylated block copolymers were developed to increase their stability and biocompatibility. The effects of the chemical structures of polycations and their PEGylation on transfection and cytotoxicity were elucidated by use of a three-dimensional multicellular tumor spheroid of human hepatoma HuH-7 cells. Various features of transfection with polyplex micelles, which have been hard to observe in conventional monolayer cultures, were revealed by the multicellular tumor spheroid (MCTS) model in terms of cytotoxicity and time-dependent behaviors of transfected gene expression under three-dimensional microenvironments. By using this system, the polyplex micelle from poly(ethylene glycol)-
b-poly(
N-substituted asparagine) copolymers having the
N-(2-aminoethyl)-2-aminoethyl group in the side chain (PEG-
b-P[Asp(DET)] polyplex micelle) was proved to achieve high transfection efficiencies as well as low cytotoxicity, both of which are critical properties for successful in vivo gene delivery.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17582637</pmid><doi>10.1016/j.jconrel.2007.05.012</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-3659 |
| ispartof | Journal of controlled release, 2007-08, Vol.121 (1), p.38-48 |
| issn | 0168-3659 1873-4995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68119046 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biocompatible Materials Biological and medical sciences Block copolymer Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell Survival Gene Expression General pharmacology Genes, Reporter Genetic Vectors Humans Hydrogen-Ion Concentration Liver Neoplasms - pathology Luciferases - metabolism Luminescent Measurements Medical sciences Micelles Nanotechnology Non-viral gene vector Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Plasmids - genetics Polyamines - chemistry Polyethylene Glycols - chemistry Polymers - chemical synthesis Polymers - chemistry Polyplex micelle Spheroid Spheroids, Cellular Transfection - methods |
| title | Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies |
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