Two-photon excited fluorescence energy transfer: a study based on oligonucleotide rulers

The use of two-photon excitation of fluorescence for detection of fluorescence resonance energy transfer (FRET) was studied for a selected fluorescent donor-acceptor pair. A method based on labeled DNA was developed for controlling the distance between the donor and the acceptor molecules. The metho...

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Veröffentlicht in:Journal of fluorescence 2006-05, Vol.16 (3), p.379-386
Hauptverfasser: Wahlroos, Rina, Toivonen, Juha, Tirri, Marko, Hänninen, Pekka
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Sprache:eng
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