Induction of immunosuppressive functions of dendritic cells in vivo by CD4+CD25+ regulatory T cells: Role of B7‐H3 expression and antigen presentation
Suppressive functions of CD4+CD25+ regulatory T cells (Treg) are mainly studied by their interaction with conventional T cells. However, there is evidence that Treg also interact with antigen‐presenting cells (APC), leading to suppression of APC function in in vitro coculture systems. Studying the i...
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| Veröffentlicht in: | European Journal of Immunology 2007-08, Vol.37 (8), p.2117-2126 |
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| container_title | European Journal of Immunology |
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| creator | Mahnke, Karsten Ring, Sabine Johnson, Theron S. Schallenberg, Sonja Schönfeld, Kurt Storn, Volker Bedke, Tanja Enk, Alexander H. |
| description | Suppressive functions of CD4+CD25+ regulatory T cells (Treg) are mainly studied by their interaction with conventional T cells. However, there is evidence that Treg also interact with antigen‐presenting cells (APC), leading to suppression of APC function in in vitro coculture systems. Studying the in vivo distribution of Treg after injection, we found that Treg are located in direct proximity to dendritic cells (DC) and affect their functional maturation status. After contact to Treg, DC up‐regulate the inhibitory B7‐H3 molecule and display reduced numbers of MHC–peptide complexes, leading to impaired T cell stimulatory function. When Treg‐exposed DC were used to immunize animals against antigens, the DC failed to produce a robust immune response as compared to control DC. Thus, these data indicate that Treg are able to inhibit DC activation and produce an inhibitory phenotype of DC. Accordingly, Treg may recruit DC for the amplification of immunosuppression by restraining their maturation in vivo and inducing an immunosuppressive phenotype of DC. |
| doi_str_mv | 10.1002/eji.200636841 |
| format | Article |
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However, there is evidence that Treg also interact with antigen‐presenting cells (APC), leading to suppression of APC function in in vitro coculture systems. Studying the in vivo distribution of Treg after injection, we found that Treg are located in direct proximity to dendritic cells (DC) and affect their functional maturation status. After contact to Treg, DC up‐regulate the inhibitory B7‐H3 molecule and display reduced numbers of MHC–peptide complexes, leading to impaired T cell stimulatory function. When Treg‐exposed DC were used to immunize animals against antigens, the DC failed to produce a robust immune response as compared to control DC. Thus, these data indicate that Treg are able to inhibit DC activation and produce an inhibitory phenotype of DC. 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| subjects | Animals Antigen presentation Antigen Presentation - immunology B7 Antigens B7-1 Antigen - immunology CD4 Antigens - immunology Cell Communication - immunology Coculture Techniques Dendritic cells Dendritic Cells - immunology Flow Cytometry Interleukin-10 - immunology Interleukin-2 Receptor alpha Subunit - immunology Lymphocyte Activation - immunology Mice T cells T-Lymphocytes, Regulatory - immunology |
| title | Induction of immunosuppressive functions of dendritic cells in vivo by CD4+CD25+ regulatory T cells: Role of B7‐H3 expression and antigen presentation |
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