Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942
ABSTRACT Carotenoid biosynthesis is up‐regulated by strong light in the cyanobacterium Synechococcus. By blocking off the pathway at the level of phytoene conversion, light‐dependent accumulation of phytoene was observed. Real‐time PCR studies demonstrated that four genes of the carotenogenic pathwa...
Gespeichert in:
| Veröffentlicht in: | Plant, cell and environment cell and environment, 2006-07, Vol.29 (7), p.1349-1356 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1356 |
|---|---|
| container_issue | 7 |
| container_start_page | 1349 |
| container_title | Plant, cell and environment |
| container_volume | 29 |
| creator | SCHÄFER, LUTZ SANDMANN, MANUELA WOITSCH, SONJA SANDMANN, GERHARD |
| description | ABSTRACT
Carotenoid biosynthesis is up‐regulated by strong light in the cyanobacterium Synechococcus. By blocking off the pathway at the level of phytoene conversion, light‐dependent accumulation of phytoene was observed. Real‐time PCR studies demonstrated that four genes of the carotenogenic pathway are under transcriptional control. These were the genes encoding phytoene synthase, phytoene desaturase, ζ‐carotene desaturase and β‐carotene hydroxylase. The transcript of the first three follow a similar kinetics, whereas the transcript of β‐carotene hydroxylase increased much faster upon transfer to high light. Promoter activities were determined with transcriptional fusions to chloramphenicol acyltransferase as reporter enzyme. The activity of the promoter of the phytoene desaturase/synthase operon was higher under strong light. |
| doi_str_mv | 10.1111/j.1365-3040.2006.01515.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68105293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68105293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5445-6f0f9065add5d20adfbd476192e161f80d38a47cd2371d725cb701d68285f6243</originalsourceid><addsrcrecordid>eNqNkc1u1DAQxy1ERZfCKyBf4JYwTvyVAwcUFYpUiUrA2fL6o-tVNl7sRO3eeASekSepw66AWxlZmtH4NzP2_BHCBGpS7O22Ji1nVQsU6gaA10AYYfX9E7T6c_EUrYBQqIToyDl6nvMWoCRE9wydEwESOsZXaOhjTDaMenJ43v_68TO523nQU4gjjh4bneLkxhgsXoeYD-O0cTlkrMvByeV9HLPDU8RDuN1MOE8ll3EY8ZfD6MwmmmjMnPFN34uONi_QmddDdi9P_gJ9-3D5tb-qrj9__NS_v64Mo5RV3IPvgDNtLbMNaOvXlgpOusYRTrwE20pNhbFNK4gVDTNrAcRy2UjmeUPbC_Tm2Hef4vfZ5UntQjZuGPTo4pwVlwRY07WPgmWk5LLtCiiPoEkx5-S82qew0-mgCKhFErVVy-bVsnm1SKJ-S6LuS-mr04x5vXP2b-FJgwK8PgE6Gz34pEcT8j9c-RftROHeHbm7MLjDfz9A3fSXS9Q-AHgYp9c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19286839</pqid></control><display><type>article</type><title>Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942</title><source>MEDLINE</source><source>Wiley Online Library</source><source>Wiley Online Library Journals</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>SCHÄFER, LUTZ ; SANDMANN, MANUELA ; WOITSCH, SONJA ; SANDMANN, GERHARD</creator><creatorcontrib>SCHÄFER, LUTZ ; SANDMANN, MANUELA ; WOITSCH, SONJA ; SANDMANN, GERHARD</creatorcontrib><description>ABSTRACT
Carotenoid biosynthesis is up‐regulated by strong light in the cyanobacterium Synechococcus. By blocking off the pathway at the level of phytoene conversion, light‐dependent accumulation of phytoene was observed. Real‐time PCR studies demonstrated that four genes of the carotenogenic pathway are under transcriptional control. These were the genes encoding phytoene synthase, phytoene desaturase, ζ‐carotene desaturase and β‐carotene hydroxylase. The transcript of the first three follow a similar kinetics, whereas the transcript of β‐carotene hydroxylase increased much faster upon transfer to high light. Promoter activities were determined with transcriptional fusions to chloramphenicol acyltransferase as reporter enzyme. The activity of the promoter of the phytoene desaturase/synthase operon was higher under strong light.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/j.1365-3040.2006.01515.x</identifier><identifier>PMID: 17080956</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - genetics ; Biological and medical sciences ; carotenogenic genes ; Carotenoids - biosynthesis ; Cell physiology ; Cyanophyta ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial - radiation effects ; Genes, Bacterial - genetics ; Light ; light regulation ; Molecular and cellular biology ; Operon - genetics ; photooxidation ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal transduction ; Synechococcus ; Synechococcus - radiation effects ; transcriptional control ; Up-Regulation - genetics ; Xanthophylls - biosynthesis ; Xanthophylls - chemistry ; Zeaxanthins</subject><ispartof>Plant, cell and environment, 2006-07, Vol.29 (7), p.1349-1356</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5445-6f0f9065add5d20adfbd476192e161f80d38a47cd2371d725cb701d68285f6243</citedby><cites>FETCH-LOGICAL-c5445-6f0f9065add5d20adfbd476192e161f80d38a47cd2371d725cb701d68285f6243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3040.2006.01515.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3040.2006.01515.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27922,27923,45572,45573,46407,46831</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17828497$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17080956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHÄFER, LUTZ</creatorcontrib><creatorcontrib>SANDMANN, MANUELA</creatorcontrib><creatorcontrib>WOITSCH, SONJA</creatorcontrib><creatorcontrib>SANDMANN, GERHARD</creatorcontrib><title>Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>ABSTRACT
Carotenoid biosynthesis is up‐regulated by strong light in the cyanobacterium Synechococcus. By blocking off the pathway at the level of phytoene conversion, light‐dependent accumulation of phytoene was observed. Real‐time PCR studies demonstrated that four genes of the carotenogenic pathway are under transcriptional control. These were the genes encoding phytoene synthase, phytoene desaturase, ζ‐carotene desaturase and β‐carotene hydroxylase. The transcript of the first three follow a similar kinetics, whereas the transcript of β‐carotene hydroxylase increased much faster upon transfer to high light. Promoter activities were determined with transcriptional fusions to chloramphenicol acyltransferase as reporter enzyme. The activity of the promoter of the phytoene desaturase/synthase operon was higher under strong light.</description><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>carotenogenic genes</subject><subject>Carotenoids - biosynthesis</subject><subject>Cell physiology</subject><subject>Cyanophyta</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial - radiation effects</subject><subject>Genes, Bacterial - genetics</subject><subject>Light</subject><subject>light regulation</subject><subject>Molecular and cellular biology</subject><subject>Operon - genetics</subject><subject>photooxidation</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal transduction</subject><subject>Synechococcus</subject><subject>Synechococcus - radiation effects</subject><subject>transcriptional control</subject><subject>Up-Regulation - genetics</subject><subject>Xanthophylls - biosynthesis</subject><subject>Xanthophylls - chemistry</subject><subject>Zeaxanthins</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAQxy1ERZfCKyBf4JYwTvyVAwcUFYpUiUrA2fL6o-tVNl7sRO3eeASekSepw66AWxlZmtH4NzP2_BHCBGpS7O22Ji1nVQsU6gaA10AYYfX9E7T6c_EUrYBQqIToyDl6nvMWoCRE9wydEwESOsZXaOhjTDaMenJ43v_68TO523nQU4gjjh4bneLkxhgsXoeYD-O0cTlkrMvByeV9HLPDU8RDuN1MOE8ll3EY8ZfD6MwmmmjMnPFN34uONi_QmddDdi9P_gJ9-3D5tb-qrj9__NS_v64Mo5RV3IPvgDNtLbMNaOvXlgpOusYRTrwE20pNhbFNK4gVDTNrAcRy2UjmeUPbC_Tm2Hef4vfZ5UntQjZuGPTo4pwVlwRY07WPgmWk5LLtCiiPoEkx5-S82qew0-mgCKhFErVVy-bVsnm1SKJ-S6LuS-mr04x5vXP2b-FJgwK8PgE6Gz34pEcT8j9c-RftROHeHbm7MLjDfz9A3fSXS9Q-AHgYp9c</recordid><startdate>200607</startdate><enddate>200607</enddate><creator>SCHÄFER, LUTZ</creator><creator>SANDMANN, MANUELA</creator><creator>WOITSCH, SONJA</creator><creator>SANDMANN, GERHARD</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200607</creationdate><title>Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942</title><author>SCHÄFER, LUTZ ; SANDMANN, MANUELA ; WOITSCH, SONJA ; SANDMANN, GERHARD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5445-6f0f9065add5d20adfbd476192e161f80d38a47cd2371d725cb701d68285f6243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>carotenogenic genes</topic><topic>Carotenoids - biosynthesis</topic><topic>Cell physiology</topic><topic>Cyanophyta</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial - radiation effects</topic><topic>Genes, Bacterial - genetics</topic><topic>Light</topic><topic>light regulation</topic><topic>Molecular and cellular biology</topic><topic>Operon - genetics</topic><topic>photooxidation</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal transduction</topic><topic>Synechococcus</topic><topic>Synechococcus - radiation effects</topic><topic>transcriptional control</topic><topic>Up-Regulation - genetics</topic><topic>Xanthophylls - biosynthesis</topic><topic>Xanthophylls - chemistry</topic><topic>Zeaxanthins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCHÄFER, LUTZ</creatorcontrib><creatorcontrib>SANDMANN, MANUELA</creatorcontrib><creatorcontrib>WOITSCH, SONJA</creatorcontrib><creatorcontrib>SANDMANN, GERHARD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SCHÄFER, LUTZ</au><au>SANDMANN, MANUELA</au><au>WOITSCH, SONJA</au><au>SANDMANN, GERHARD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2006-07</date><risdate>2006</risdate><volume>29</volume><issue>7</issue><spage>1349</spage><epage>1356</epage><pages>1349-1356</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>ABSTRACT
Carotenoid biosynthesis is up‐regulated by strong light in the cyanobacterium Synechococcus. By blocking off the pathway at the level of phytoene conversion, light‐dependent accumulation of phytoene was observed. Real‐time PCR studies demonstrated that four genes of the carotenogenic pathway are under transcriptional control. These were the genes encoding phytoene synthase, phytoene desaturase, ζ‐carotene desaturase and β‐carotene hydroxylase. The transcript of the first three follow a similar kinetics, whereas the transcript of β‐carotene hydroxylase increased much faster upon transfer to high light. Promoter activities were determined with transcriptional fusions to chloramphenicol acyltransferase as reporter enzyme. The activity of the promoter of the phytoene desaturase/synthase operon was higher under strong light.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17080956</pmid><doi>10.1111/j.1365-3040.2006.01515.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0140-7791 |
| ispartof | Plant, cell and environment, 2006-07, Vol.29 (7), p.1349-1356 |
| issn | 0140-7791 1365-3040 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68105293 |
| source | MEDLINE; Wiley Online Library; Wiley Online Library Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Bacterial Proteins - genetics Biological and medical sciences carotenogenic genes Carotenoids - biosynthesis Cell physiology Cyanophyta Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - radiation effects Genes, Bacterial - genetics Light light regulation Molecular and cellular biology Operon - genetics photooxidation Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Signal transduction Synechococcus Synechococcus - radiation effects transcriptional control Up-Regulation - genetics Xanthophylls - biosynthesis Xanthophylls - chemistry Zeaxanthins |
| title | Coordinate up‐regulation of carotenoid biosynthesis as a response to light stress in Synechococcus PCC7942 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T15%3A54%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Coordinate%20up%E2%80%90regulation%20of%20carotenoid%20biosynthesis%20as%20a%20response%20to%20light%20stress%20in%20Synechococcus%20PCC7942&rft.jtitle=Plant,%20cell%20and%20environment&rft.au=SCH%C3%84FER,%20LUTZ&rft.date=2006-07&rft.volume=29&rft.issue=7&rft.spage=1349&rft.epage=1356&rft.pages=1349-1356&rft.issn=0140-7791&rft.eissn=1365-3040&rft.coden=PLCEDV&rft_id=info:doi/10.1111/j.1365-3040.2006.01515.x&rft_dat=%3Cproquest_cross%3E68105293%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19286839&rft_id=info:pmid/17080956&rfr_iscdi=true |