Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca)
In addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (OGA), are essential components for elicitation of defence responses. To investigate how modifications of pectin alter defence responses, we expresse...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2008-04, Vol.54 (1), p.43-55 |
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| creator | Osorio, Sonia Castillejo, Cristina Quesada, Miguel A Medina-Escobar, Nieves Brownsey, Geoff J Suau, Rafael Heredia, Antonio Botella, Miguel A Valpuesta, Victoriano |
| description | In addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (OGA), are essential components for elicitation of defence responses. To investigate how modifications of pectin alter defence responses, we expressed the fruit-specific Fragaria x ananassa pectin methyl esterase FaPE1 in the wild strawberry Fragaria vesca. Pectin from transgenic ripe fruits differed from the wild-type with regard to the degree and pattern of methyl esterification, as well as the average size of pectin polymers. Purified oligogalacturonides from the transgenic fruits showed a reduced degree of esterification compared to oligogalacturonides from wild-type fruits. This reduced esterification is necessary to elicit defence responses in strawberry. The transgenic F. vesca lines had constitutively activated pathogen defence responses, resulting in higher resistance to the necrotropic fungus Botrytis cinerea. Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged. |
| doi_str_mv | 10.1111/j.1365-313X.2007.03398.x |
| format | Article |
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To investigate how modifications of pectin alter defence responses, we expressed the fruit-specific Fragaria x ananassa pectin methyl esterase FaPE1 in the wild strawberry Fragaria vesca. Pectin from transgenic ripe fruits differed from the wild-type with regard to the degree and pattern of methyl esterification, as well as the average size of pectin polymers. Purified oligogalacturonides from the transgenic fruits showed a reduced degree of esterification compared to oligogalacturonides from wild-type fruits. This reduced esterification is necessary to elicit defence responses in strawberry. The transgenic F. vesca lines had constitutively activated pathogen defence responses, resulting in higher resistance to the necrotropic fungus Botrytis cinerea. Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313X.2007.03398.x</identifier><identifier>PMID: 18088306</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Botany ; Botrytis - physiology ; Carboxylic Ester Hydrolases - metabolism ; cell wall ; defence ; Enzymes ; esterification ; Flowers & plants ; Fragaria - metabolism ; Fruit - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant - physiology ; oligogalacturonides ; Pathology. Damages, economic importance ; pectin methyl esterase ; pectinesterase ; Pectins - chemistry ; Pectins - metabolism ; Phytopathology. Animal pests. 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To investigate how modifications of pectin alter defence responses, we expressed the fruit-specific Fragaria x ananassa pectin methyl esterase FaPE1 in the wild strawberry Fragaria vesca. Pectin from transgenic ripe fruits differed from the wild-type with regard to the degree and pattern of methyl esterification, as well as the average size of pectin polymers. Purified oligogalacturonides from the transgenic fruits showed a reduced degree of esterification compared to oligogalacturonides from wild-type fruits. This reduced esterification is necessary to elicit defence responses in strawberry. The transgenic F. vesca lines had constitutively activated pathogen defence responses, resulting in higher resistance to the necrotropic fungus Botrytis cinerea. Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged.</description><subject>Biological and medical sciences</subject><subject>Botany</subject><subject>Botrytis - physiology</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>cell wall</subject><subject>defence</subject><subject>Enzymes</subject><subject>esterification</subject><subject>Flowers & plants</subject><subject>Fragaria - metabolism</subject><subject>Fruit - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>oligogalacturonides</subject><subject>Pathology. Damages, economic importance</subject><subject>pectin methyl esterase</subject><subject>pectinesterase</subject><subject>Pectins - chemistry</subject><subject>Pectins - metabolism</subject><subject>Phytopathology. 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Plant and forest protection</subject><subject>Plant Diseases - microbiology</subject><subject>Plant pathology</subject><subject>Plant Proteins - metabolism</subject><subject>Plant viruses and viroids</subject><subject>Plants, Genetically Modified</subject><subject>strawberries</subject><subject>strawberry</subject><subject>Uronic Acids - metabolism</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1TAQhSMEoreFVwALCQSLBDtOHGfRBapaflSJSrQSO2uSjIOvfONbO6HNm_C4OOSqSKzwxpbmO8czc5KEMJqxeN5vM8ZFmXLGv2c5pVVGOa9ldv8o2TwUHicbWguaVgXLj5LjELaUsoqL4mlyxCSVklOxSX5dgR8NWNLhDscfs4XRuIE4TZw1vevBQjtO3g2mw0CameyxHc1AVphgGNFDQMKICcTj7WQ8dkQ7T9Ca1kS0j9YahxZjOezdEKJPNLgztiNh9HDXoPczeXvhoQdvgPzE0MK7Z8kTDTbg88N9ktxcnF-ffUovv378fPbhMm2LWsq0qrGohW6KgklglAGtedOVoinbDmrgmnW0qlmRMykjpXkhQbIaAApeio7yk-TN6rv37naK86idCS1aCwO6KSghGeVCsAi--gfcuskPsTeVM17GBMoFkivUeheCR6323uzAz4pRtUSntmpJSC0JqSU69Sc6dR-lLw7-U7PD7q_wkFUEXh8AiAuy2sPQmvDA5TTPeV4vE52uXFwxzv_dgLq--rK8ov7lqtfgFPQ-_nHzLaeMUyqrQnLOfwMZpsAZ</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Osorio, Sonia</creator><creator>Castillejo, Cristina</creator><creator>Quesada, Miguel A</creator><creator>Medina-Escobar, Nieves</creator><creator>Brownsey, Geoff J</creator><creator>Suau, Rafael</creator><creator>Heredia, Antonio</creator><creator>Botella, Miguel A</creator><creator>Valpuesta, Victoriano</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca)</title><author>Osorio, Sonia ; Castillejo, Cristina ; Quesada, Miguel A ; Medina-Escobar, Nieves ; Brownsey, Geoff J ; Suau, Rafael ; Heredia, Antonio ; Botella, Miguel A ; Valpuesta, Victoriano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4988-79e496fb4418a101a093bd56b5cda9a3f1d079142188fb4f348a819aaa4356d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Botany</topic><topic>Botrytis - physiology</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>cell wall</topic><topic>defence</topic><topic>Enzymes</topic><topic>esterification</topic><topic>Flowers & plants</topic><topic>Fragaria - metabolism</topic><topic>Fruit - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>oligogalacturonides</topic><topic>Pathology. Damages, economic importance</topic><topic>pectin methyl esterase</topic><topic>pectinesterase</topic><topic>Pectins - chemistry</topic><topic>Pectins - metabolism</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases - microbiology</topic><topic>Plant pathology</topic><topic>Plant Proteins - metabolism</topic><topic>Plant viruses and viroids</topic><topic>Plants, Genetically Modified</topic><topic>strawberries</topic><topic>strawberry</topic><topic>Uronic Acids - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osorio, Sonia</creatorcontrib><creatorcontrib>Castillejo, Cristina</creatorcontrib><creatorcontrib>Quesada, Miguel A</creatorcontrib><creatorcontrib>Medina-Escobar, Nieves</creatorcontrib><creatorcontrib>Brownsey, Geoff J</creatorcontrib><creatorcontrib>Suau, Rafael</creatorcontrib><creatorcontrib>Heredia, Antonio</creatorcontrib><creatorcontrib>Botella, Miguel A</creatorcontrib><creatorcontrib>Valpuesta, Victoriano</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osorio, Sonia</au><au>Castillejo, Cristina</au><au>Quesada, Miguel A</au><au>Medina-Escobar, Nieves</au><au>Brownsey, Geoff J</au><au>Suau, Rafael</au><au>Heredia, Antonio</au><au>Botella, Miguel A</au><au>Valpuesta, Victoriano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca)</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2008-04</date><risdate>2008</risdate><volume>54</volume><issue>1</issue><spage>43</spage><epage>55</epage><pages>43-55</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>In addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (OGA), are essential components for elicitation of defence responses. To investigate how modifications of pectin alter defence responses, we expressed the fruit-specific Fragaria x ananassa pectin methyl esterase FaPE1 in the wild strawberry Fragaria vesca. Pectin from transgenic ripe fruits differed from the wild-type with regard to the degree and pattern of methyl esterification, as well as the average size of pectin polymers. Purified oligogalacturonides from the transgenic fruits showed a reduced degree of esterification compared to oligogalacturonides from wild-type fruits. This reduced esterification is necessary to elicit defence responses in strawberry. The transgenic F. vesca lines had constitutively activated pathogen defence responses, resulting in higher resistance to the necrotropic fungus Botrytis cinerea. Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18088306</pmid><doi>10.1111/j.1365-313X.2007.03398.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Botany Botrytis - physiology Carboxylic Ester Hydrolases - metabolism cell wall defence Enzymes esterification Flowers & plants Fragaria - metabolism Fruit - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - physiology oligogalacturonides Pathology. Damages, economic importance pectin methyl esterase pectinesterase Pectins - chemistry Pectins - metabolism Phytopathology. Animal pests. Plant and forest protection Plant Diseases - microbiology Plant pathology Plant Proteins - metabolism Plant viruses and viroids Plants, Genetically Modified strawberries strawberry Uronic Acids - metabolism |
| title | Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca) |
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