A Novel Approach to Analyze Membrane Proteins by Laser Mass Spectrometry: From Protein Subunits to the Integral Complex

A novel laser-based mass spectrometry method termed LILBID (laser-induced liquid bead ion desorption) is applied to analyze large integral membrane protein complexes and their subunits. In this method the ions are IR-laser desorbed from aqueous microdroplets containing the hydrophobic protein comple...

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Veröffentlicht in:	Journal of the American Society for Mass Spectrometry 2007-08, Vol.18 (8), p.1429-1438
Hauptverfasser:	Morgner, Nina, Kleinschroth, Thomas, Barth, Hans-Dieter, Ludwig, Bernd, Brutschy, Bernhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Charge distribution Cytochrome Desorption Electron Transport Complex III - chemistry Electron Transport Complex IV - chemistry Fundamental and applied biological sciences. Psychology General aspects, investigation methods Helices Integrals Iridium Laser applications Lasers Mass spectrometry Mass Spectrometry - methods Membrane Proteins - analysis Membrane Proteins - chemistry Models, Molecular Oligomerization Paracoccus denitrificans - chemistry Paracoccus denitrificans - metabolism Polypeptides Protein Subunits - analysis Protein Subunits - chemistry Proteins Scientific imaging Spectroscopy
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