Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature
This research project tested the hypothesis that cold-equilibrated (∼0°C) human erythrocytes in vitro in the presence of an ultrasound contrast agent (Albunex ®) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37°C) human erythrocytes in vitro because of a temper...
Gespeichert in:
| Veröffentlicht in: | Ultrasound in medicine & biology 2006-06, Vol.32 (6), p.893-904 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 904 |
|---|---|
| container_issue | 6 |
| container_start_page | 893 |
| container_title | Ultrasound in medicine & biology |
| container_volume | 32 |
| creator | Miller, Morton W. Church, Charles C. Labuda, Cecille Mazza, Salvatore Raymond, Jason |
| description | This research project tested the hypothesis that cold-equilibrated (∼0°C) human erythrocytes
in vitro in the presence of an ultrasound contrast agent (Albunex
®) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37°C) human erythrocytes
in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 μs pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P
−) and (2) comparable viscosities of the 0 and 37°C blood plasmas, the cold (∼0°C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37°C) erythrocytes. The data supported the hypothesis. (E-mail:
Morton_Miller@urmc.rochester.edu) |
| doi_str_mv | 10.1016/j.ultrasmedbio.2006.02.1423 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68098476</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0301562906015055</els_id><sourcerecordid>68098476</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-45ff3b1c47b4c2b41c7e840d49e79297a5c45f6e2cf2f224af7a014249b4cb4b3</originalsourceid><addsrcrecordid>eNqNkUFrHCEYhqUkJJs0f6EIhd5moo6jY3NqQpoEArkk0Js4zmfqMqNbnVnIv6_LLqS35iTo8_nyPS9CXympKaHicl0v45xMnmDofawZIaImrKacNZ_QinZSVUzRX0doRRpCq1YwdYrOcl4TQqRo5Ak6pUJ2LaF0heDaxzG-emtGbMKAIWx9imGCMJcbZ-wcU8bGObCzD694Hx2XMFQ-DIuFAf-GKY5v2WfsA976OUX8Hbc1foZpA8nMS4LP6NiZMcPF4TxHLz9vn2_uq8enu4ebH4-V5ZTNFW-da3pquey5ZT2nVkLHycAVSMWUNK0tiABmHXOMceOkIWVvrgrf8745R9_2_25S_LNAnvXks4VxNAHikrXoiOp4kfA_kCrOhRJdAa_2oE0x5wROb5KfTHrTlOhdHXqt_61D7-rQhOldHWX6yyFm6cv7--zBfwFu9wAUK1sPSWfrIRStPhXjeoj-Q0F_AaNvpPM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19446968</pqid></control><display><type>article</type><title>Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Miller, Morton W. ; Church, Charles C. ; Labuda, Cecille ; Mazza, Salvatore ; Raymond, Jason</creator><creatorcontrib>Miller, Morton W. ; Church, Charles C. ; Labuda, Cecille ; Mazza, Salvatore ; Raymond, Jason</creatorcontrib><description>This research project tested the hypothesis that cold-equilibrated (∼0°C) human erythrocytes
in vitro in the presence of an ultrasound contrast agent (Albunex
®) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37°C) human erythrocytes
in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 μs pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P
−) and (2) comparable viscosities of the 0 and 37°C blood plasmas, the cold (∼0°C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37°C) erythrocytes. The data supported the hypothesis. (E-mail:
Morton_Miller@urmc.rochester.edu)</description><identifier>ISSN: 0301-5629</identifier><identifier>EISSN: 1879-291X</identifier><identifier>DOI: 10.1016/j.ultrasmedbio.2006.02.1423</identifier><identifier>PMID: 16785011</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adult ; Blood in vitro ; Blood Viscosity ; Erythrocyte Membrane - physiology ; Hemolysis ; Humans ; In Vitro Techniques ; Oxygen - blood ; Partial Pressure ; pO 2 levels ; Reciprocity calibration ; Temperature ; Transducers ; Ultrasonography ; Ultrasound ; Viscosity</subject><ispartof>Ultrasound in medicine & biology, 2006-06, Vol.32 (6), p.893-904</ispartof><rights>2006 World Federation for Ultrasound in Medicine & Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-45ff3b1c47b4c2b41c7e840d49e79297a5c45f6e2cf2f224af7a014249b4cb4b3</citedby><cites>FETCH-LOGICAL-c412t-45ff3b1c47b4c2b41c7e840d49e79297a5c45f6e2cf2f224af7a014249b4cb4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0301562906015055$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16785011$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, Morton W.</creatorcontrib><creatorcontrib>Church, Charles C.</creatorcontrib><creatorcontrib>Labuda, Cecille</creatorcontrib><creatorcontrib>Mazza, Salvatore</creatorcontrib><creatorcontrib>Raymond, Jason</creatorcontrib><title>Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature</title><title>Ultrasound in medicine & biology</title><addtitle>Ultrasound Med Biol</addtitle><description>This research project tested the hypothesis that cold-equilibrated (∼0°C) human erythrocytes
in vitro in the presence of an ultrasound contrast agent (Albunex
®) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37°C) human erythrocytes
in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 μs pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P
−) and (2) comparable viscosities of the 0 and 37°C blood plasmas, the cold (∼0°C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37°C) erythrocytes. The data supported the hypothesis. (E-mail:
Morton_Miller@urmc.rochester.edu)</description><subject>Adult</subject><subject>Blood in vitro</subject><subject>Blood Viscosity</subject><subject>Erythrocyte Membrane - physiology</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Oxygen - blood</subject><subject>Partial Pressure</subject><subject>pO 2 levels</subject><subject>Reciprocity calibration</subject><subject>Temperature</subject><subject>Transducers</subject><subject>Ultrasonography</subject><subject>Ultrasound</subject><subject>Viscosity</subject><issn>0301-5629</issn><issn>1879-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFrHCEYhqUkJJs0f6EIhd5moo6jY3NqQpoEArkk0Js4zmfqMqNbnVnIv6_LLqS35iTo8_nyPS9CXympKaHicl0v45xMnmDofawZIaImrKacNZ_QinZSVUzRX0doRRpCq1YwdYrOcl4TQqRo5Ak6pUJ2LaF0heDaxzG-emtGbMKAIWx9imGCMJcbZ-wcU8bGObCzD694Hx2XMFQ-DIuFAf-GKY5v2WfsA976OUX8Hbc1foZpA8nMS4LP6NiZMcPF4TxHLz9vn2_uq8enu4ebH4-V5ZTNFW-da3pquey5ZT2nVkLHycAVSMWUNK0tiABmHXOMceOkIWVvrgrf8745R9_2_25S_LNAnvXks4VxNAHikrXoiOp4kfA_kCrOhRJdAa_2oE0x5wROb5KfTHrTlOhdHXqt_61D7-rQhOldHWX6yyFm6cv7--zBfwFu9wAUK1sPSWfrIRStPhXjeoj-Q0F_AaNvpPM</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Miller, Morton W.</creator><creator>Church, Charles C.</creator><creator>Labuda, Cecille</creator><creator>Mazza, Salvatore</creator><creator>Raymond, Jason</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature</title><author>Miller, Morton W. ; Church, Charles C. ; Labuda, Cecille ; Mazza, Salvatore ; Raymond, Jason</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-45ff3b1c47b4c2b41c7e840d49e79297a5c45f6e2cf2f224af7a014249b4cb4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Blood in vitro</topic><topic>Blood Viscosity</topic><topic>Erythrocyte Membrane - physiology</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Oxygen - blood</topic><topic>Partial Pressure</topic><topic>pO 2 levels</topic><topic>Reciprocity calibration</topic><topic>Temperature</topic><topic>Transducers</topic><topic>Ultrasonography</topic><topic>Ultrasound</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Morton W.</creatorcontrib><creatorcontrib>Church, Charles C.</creatorcontrib><creatorcontrib>Labuda, Cecille</creatorcontrib><creatorcontrib>Mazza, Salvatore</creatorcontrib><creatorcontrib>Raymond, Jason</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Ultrasound in medicine & biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Morton W.</au><au>Church, Charles C.</au><au>Labuda, Cecille</au><au>Mazza, Salvatore</au><au>Raymond, Jason</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature</atitle><jtitle>Ultrasound in medicine & biology</jtitle><addtitle>Ultrasound Med Biol</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>32</volume><issue>6</issue><spage>893</spage><epage>904</epage><pages>893-904</pages><issn>0301-5629</issn><eissn>1879-291X</eissn><abstract>This research project tested the hypothesis that cold-equilibrated (∼0°C) human erythrocytes
in vitro in the presence of an ultrasound contrast agent (Albunex
®) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37°C) human erythrocytes
in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 μs pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P
−) and (2) comparable viscosities of the 0 and 37°C blood plasmas, the cold (∼0°C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37°C) erythrocytes. The data supported the hypothesis. (E-mail:
Morton_Miller@urmc.rochester.edu)</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>16785011</pmid><doi>10.1016/j.ultrasmedbio.2006.02.1423</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-5629 |
| ispartof | Ultrasound in medicine & biology, 2006-06, Vol.32 (6), p.893-904 |
| issn | 0301-5629 1879-291X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68098476 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Blood in vitro Blood Viscosity Erythrocyte Membrane - physiology Hemolysis Humans In Vitro Techniques Oxygen - blood Partial Pressure pO 2 levels Reciprocity calibration Temperature Transducers Ultrasonography Ultrasound Viscosity |
| title | Biological and environmental factors affecting ultrasound-induced hemolysis in vitro : 5. Temperature |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T11%3A55%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biological%20and%20environmental%20factors%20affecting%20ultrasound-induced%20hemolysis%20in%20vitro%20:%205.%20Temperature&rft.jtitle=Ultrasound%20in%20medicine%20&%20biology&rft.au=Miller,%20Morton%20W.&rft.date=2006-06-01&rft.volume=32&rft.issue=6&rft.spage=893&rft.epage=904&rft.pages=893-904&rft.issn=0301-5629&rft.eissn=1879-291X&rft_id=info:doi/10.1016/j.ultrasmedbio.2006.02.1423&rft_dat=%3Cproquest_cross%3E68098476%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19446968&rft_id=info:pmid/16785011&rft_els_id=S0301562906015055&rfr_iscdi=true |