Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates
Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 10...
Gespeichert in:
| Veröffentlicht in: | International journal for parasitology 2006-06, Vol.36 (7), p.849-858 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 858 |
|---|---|
| container_issue | 7 |
| container_start_page | 849 |
| container_title | International journal for parasitology |
| container_volume | 36 |
| creator | van der Giessen, J.W.B. de Vries, A. Roos, M. Wielinga, Peter Kortbeek, L.M. Mank, T.G. |
| description | Giardia duodenalis (syn.
Giardia lamblia,
Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of
G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100
Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different
G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the
G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (
gdh) genes. Genotyping results showed that
G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these
Giardia and those available in GenBank. Using
gdh DNA sequence analysis, human and animal Assemblage A and B
Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host–species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor. |
| doi_str_mv | 10.1016/j.ijpara.2006.03.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68097653</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0020751906000816</els_id><sourcerecordid>68097653</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-5c5aadc661b3a674d3450c2959ae903af9edce61f0271c52d2bbc819307814673</originalsourceid><addsrcrecordid>eNqF0UFv0zAUB3ALMbEy-AYIcoFbwrMd2wmHSdOAbtIkDrCz9eo4ras0DraD1G-Pq1QaJ3awLFm_92y_PyHvKFQUqPy8r9x-woAVA5AV8AqAviAr2qi2BMrFS7ICYFAqQdtL8jrGfQaC1_Urckmlyi0kXxG3tqNPx8mN28L3xdph6BwWbiy-zsnsigmTs2OKBY5dXu6AQ_xS3BTT7jj4rR1tciaf43CMLp467OYDjv_owkU_YLLxDbnoc7F9e96vyOP3b79u78qHH-v725uH0gihUimMQOyMlHTDUaq647UAw1rRom2BY9_azlhJe2CKGsE6ttmYhrYcVENrqfgV-bT0nYL_PduY9MFFY4cBR-vnqGUDrZKCPwupYhRqJTOsF2iCjzHYXk8hfy0cNQV9ykLv9ZKFPmWhges86lz2_tx_3hxs91R0Hn4GH88Ao8GhDzgaF5-capqTzO7D4nr0Grchm8efLN8AFBpBa5bF9SJsHuwfZ4OOJsdmbOeCNUl33v3_rX8BXDyyRA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17210476</pqid></control><display><type>article</type><title>Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>van der Giessen, J.W.B. ; de Vries, A. ; Roos, M. ; Wielinga, Peter ; Kortbeek, L.M. ; Mank, T.G.</creator><creatorcontrib>van der Giessen, J.W.B. ; de Vries, A. ; Roos, M. ; Wielinga, Peter ; Kortbeek, L.M. ; Mank, T.G.</creatorcontrib><description>Giardia duodenalis (syn.
Giardia lamblia,
Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of
G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100
Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different
G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the
G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (
gdh) genes. Genotyping results showed that
G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these
Giardia and those available in GenBank. Using
gdh DNA sequence analysis, human and animal Assemblage A and B
Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host–species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2006.03.001</identifier><identifier>PMID: 16701663</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>18S rRNA ; Animals ; Base Sequence ; Biological and medical sciences ; Databases, Nucleic Acid ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Protozoan ; genetic variation ; Genotype ; Giardia ; Giardia duodenalis ; Giardia intestinalis ; Giardia lamblia ; Giardia lamblia - classification ; Giardia lamblia - genetics ; Giardia lamblia - isolation & purification ; giardiasis ; Giardiasis - parasitology ; Giardiasis - transmission ; Giardiasis - veterinary ; Glutamate dehydrogenase ; Glutamate Dehydrogenase - genetics ; glutamate dehydrogenase gene ; host strains ; human strains ; Humans ; Life cycle. Host-agent relationship. Pathogenesis ; mammalian strains ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequences ; Phylogenetic analysis ; Phylogeny ; polymerase chain reaction ; Protozoa ; restriction fragment length polymorphism ; ribosomal DNA ; RNA, Ribosomal, 18S - genetics ; sequence homology ; Species Specificity ; strain differences ; zoonoses ; Zoonoses - transmission ; Zoonotic disease</subject><ispartof>International journal for parasitology, 2006-06, Vol.36 (7), p.849-858</ispartof><rights>2006 Australian Society for Parasitology Inc</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-5c5aadc661b3a674d3450c2959ae903af9edce61f0271c52d2bbc819307814673</citedby><cites>FETCH-LOGICAL-c557t-5c5aadc661b3a674d3450c2959ae903af9edce61f0271c52d2bbc819307814673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020751906000816$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17887016$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16701663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Giessen, J.W.B.</creatorcontrib><creatorcontrib>de Vries, A.</creatorcontrib><creatorcontrib>Roos, M.</creatorcontrib><creatorcontrib>Wielinga, Peter</creatorcontrib><creatorcontrib>Kortbeek, L.M.</creatorcontrib><creatorcontrib>Mank, T.G.</creatorcontrib><title>Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Giardia duodenalis (syn.
Giardia lamblia,
Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of
G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100
Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different
G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the
G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (
gdh) genes. Genotyping results showed that
G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these
Giardia and those available in GenBank. Using
gdh DNA sequence analysis, human and animal Assemblage A and B
Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host–species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor.</description><subject>18S rRNA</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Databases, Nucleic Acid</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Protozoan</subject><subject>genetic variation</subject><subject>Genotype</subject><subject>Giardia</subject><subject>Giardia duodenalis</subject><subject>Giardia intestinalis</subject><subject>Giardia lamblia</subject><subject>Giardia lamblia - classification</subject><subject>Giardia lamblia - genetics</subject><subject>Giardia lamblia - isolation & purification</subject><subject>giardiasis</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - transmission</subject><subject>Giardiasis - veterinary</subject><subject>Glutamate dehydrogenase</subject><subject>Glutamate Dehydrogenase - genetics</subject><subject>glutamate dehydrogenase gene</subject><subject>host strains</subject><subject>human strains</subject><subject>Humans</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>mammalian strains</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Protozoa</subject><subject>restriction fragment length polymorphism</subject><subject>ribosomal DNA</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>sequence homology</subject><subject>Species Specificity</subject><subject>strain differences</subject><subject>zoonoses</subject><subject>Zoonoses - transmission</subject><subject>Zoonotic disease</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFv0zAUB3ALMbEy-AYIcoFbwrMd2wmHSdOAbtIkDrCz9eo4ras0DraD1G-Pq1QaJ3awLFm_92y_PyHvKFQUqPy8r9x-woAVA5AV8AqAviAr2qi2BMrFS7ICYFAqQdtL8jrGfQaC1_Urckmlyi0kXxG3tqNPx8mN28L3xdph6BwWbiy-zsnsigmTs2OKBY5dXu6AQ_xS3BTT7jj4rR1tciaf43CMLp467OYDjv_owkU_YLLxDbnoc7F9e96vyOP3b79u78qHH-v725uH0gihUimMQOyMlHTDUaq647UAw1rRom2BY9_azlhJe2CKGsE6ttmYhrYcVENrqfgV-bT0nYL_PduY9MFFY4cBR-vnqGUDrZKCPwupYhRqJTOsF2iCjzHYXk8hfy0cNQV9ykLv9ZKFPmWhges86lz2_tx_3hxs91R0Hn4GH88Ao8GhDzgaF5-capqTzO7D4nr0Grchm8efLN8AFBpBa5bF9SJsHuwfZ4OOJsdmbOeCNUl33v3_rX8BXDyyRA</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>van der Giessen, J.W.B.</creator><creator>de Vries, A.</creator><creator>Roos, M.</creator><creator>Wielinga, Peter</creator><creator>Kortbeek, L.M.</creator><creator>Mank, T.G.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates</title><author>van der Giessen, J.W.B. ; de Vries, A. ; Roos, M. ; Wielinga, Peter ; Kortbeek, L.M. ; Mank, T.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-5c5aadc661b3a674d3450c2959ae903af9edce61f0271c52d2bbc819307814673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>18S rRNA</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Databases, Nucleic Acid</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Protozoan</topic><topic>genetic variation</topic><topic>Genotype</topic><topic>Giardia</topic><topic>Giardia duodenalis</topic><topic>Giardia intestinalis</topic><topic>Giardia lamblia</topic><topic>Giardia lamblia - classification</topic><topic>Giardia lamblia - genetics</topic><topic>Giardia lamblia - isolation & purification</topic><topic>giardiasis</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - transmission</topic><topic>Giardiasis - veterinary</topic><topic>Glutamate dehydrogenase</topic><topic>Glutamate Dehydrogenase - genetics</topic><topic>glutamate dehydrogenase gene</topic><topic>host strains</topic><topic>human strains</topic><topic>Humans</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>mammalian strains</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Protozoa</topic><topic>restriction fragment length polymorphism</topic><topic>ribosomal DNA</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>sequence homology</topic><topic>Species Specificity</topic><topic>strain differences</topic><topic>zoonoses</topic><topic>Zoonoses - transmission</topic><topic>Zoonotic disease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Giessen, J.W.B.</creatorcontrib><creatorcontrib>de Vries, A.</creatorcontrib><creatorcontrib>Roos, M.</creatorcontrib><creatorcontrib>Wielinga, Peter</creatorcontrib><creatorcontrib>Kortbeek, L.M.</creatorcontrib><creatorcontrib>Mank, T.G.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Giessen, J.W.B.</au><au>de Vries, A.</au><au>Roos, M.</au><au>Wielinga, Peter</au><au>Kortbeek, L.M.</au><au>Mank, T.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>36</volume><issue>7</issue><spage>849</spage><epage>858</epage><pages>849-858</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Giardia duodenalis (syn.
Giardia lamblia,
Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of
G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100
Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different
G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the
G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (
gdh) genes. Genotyping results showed that
G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these
Giardia and those available in GenBank. Using
gdh DNA sequence analysis, human and animal Assemblage A and B
Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host–species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16701663</pmid><doi>10.1016/j.ijpara.2006.03.001</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-7519 |
| ispartof | International journal for parasitology, 2006-06, Vol.36 (7), p.849-858 |
| issn | 0020-7519 1879-0135 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68097653 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 18S rRNA Animals Base Sequence Biological and medical sciences Databases, Nucleic Acid DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology Genes, Protozoan genetic variation Genotype Giardia Giardia duodenalis Giardia intestinalis Giardia lamblia Giardia lamblia - classification Giardia lamblia - genetics Giardia lamblia - isolation & purification giardiasis Giardiasis - parasitology Giardiasis - transmission Giardiasis - veterinary Glutamate dehydrogenase Glutamate Dehydrogenase - genetics glutamate dehydrogenase gene host strains human strains Humans Life cycle. Host-agent relationship. Pathogenesis mammalian strains Molecular genetics Molecular Sequence Data nucleotide sequences Phylogenetic analysis Phylogeny polymerase chain reaction Protozoa restriction fragment length polymorphism ribosomal DNA RNA, Ribosomal, 18S - genetics sequence homology Species Specificity strain differences zoonoses Zoonoses - transmission Zoonotic disease |
| title | Genotyping of Giardia in Dutch patients and animals: A phylogenetic analysis of human and animal isolates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A34%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotyping%20of%20Giardia%20in%20Dutch%20patients%20and%20animals:%20A%20phylogenetic%20analysis%20of%20human%20and%20animal%20isolates&rft.jtitle=International%20journal%20for%20parasitology&rft.au=van%20der%20Giessen,%20J.W.B.&rft.date=2006-06-01&rft.volume=36&rft.issue=7&rft.spage=849&rft.epage=858&rft.pages=849-858&rft.issn=0020-7519&rft.eissn=1879-0135&rft.coden=IJPYBT&rft_id=info:doi/10.1016/j.ijpara.2006.03.001&rft_dat=%3Cproquest_cross%3E68097653%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17210476&rft_id=info:pmid/16701663&rft_els_id=S0020751906000816&rfr_iscdi=true |