Enhancement of transient gene expression by fed-batch culture of HEK 293 EBNA1 cells in suspension

Enhanced green fluorescence protein (GFP) and erythropoietin (EPO) were used as reporters to assess and improve transient gene expression in HEK 293 EBNA1 cells. The production of EPO only lasted 3 days and reached 18.1 mg/l in suspension cultures in 1 l batch bioreactors. However, GFP expression ex...

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Veröffentlicht in:	Biotechnology letters 2006-06, Vol.28 (11), p.843-848
Hauptverfasser:	Sun, Xiangming, Goh, Peen Ern, Wong, Kathy T. K, Mori, Tetsushi, Yap, Miranda G. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Batch culture Biological and medical sciences Bioreactors Biotechnology Cell culture Cell Culture Techniques - methods Cell Line Culture Media, Serum-Free - metabolism Erythropoietin Erythropoietin - biosynthesis Erythropoietin - genetics Fed batch Fed-batch culture Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - physiology Gene Expression Regulation Genes, Reporter - physiology Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics HEK 293 EBNA1 cells Humans Kidney - cytology Kidney - embryology Polyethylenimine Proteins Recombinant Proteins - biosynthesis Serum-free medium Transfection Transfection - methods Transient gene expression
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