Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain
In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells f...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular neuroscience 2006-05, Vol.32 (1), p.187-198 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 198 |
|---|---|
| container_issue | 1 |
| container_start_page | 187 |
| container_title | Molecular and cellular neuroscience |
| container_volume | 32 |
| creator | Seidenfaden, Ralph Desoeuvre, Angélique Bosio, Andreas Virard, Isabelle Cremer, Harold |
| description | In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5
+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment. |
| doi_str_mv | 10.1016/j.mcn.2006.04.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68090926</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1044743106000753</els_id><sourcerecordid>17264407</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-e76119f6508b88c247d12074bd9a651bec04f841592d8fb68ba39b21c63885243</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EoqXwAbggn7gljP_EscUJVbRFqtRDgQMXK3EmxavEXmxnJb49Xu1K3OA0bzS_eYf3CHnLoGXA1Iddu7rQcgDVgmwBxDNyycB0jRG8f37UUja9FOyCvMp5BwAdN-IluWCqFyA7dUn87eKHhboYDpiyj4HGmT5-_9FMmPwBp3pZV19KVQG3FEOF9wndlnJMmQ5zwUTRlbj3jj6luvvwRH0okZafSIdpWwod0-DDa_JiHpaMb87ziny7-fz1-q65f7j9cv3pvnFC89Jgrxgzs-pAj1o7LvuJcejlOJlBdWxEB3LWknWGT3oelR4HYUbOnBJad1yKK_L-5LtP8deGudjVZ4fLMgSMW7ZKgwHD1X9B1nMlJfQVZCfQpZhzwtnuk1-H9NsysMci7M7WIuyxCAvS1iLqz7uz-TauOP39OCdfgY8nAGsWB4_JZucxOJx8jbfYKfp_2P8BzcGZIQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17264407</pqid></control><display><type>article</type><title>Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Seidenfaden, Ralph ; Desoeuvre, Angélique ; Bosio, Andreas ; Virard, Isabelle ; Cremer, Harold</creator><creatorcontrib>Seidenfaden, Ralph ; Desoeuvre, Angélique ; Bosio, Andreas ; Virard, Isabelle ; Cremer, Harold</creatorcontrib><description>In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5
+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1016/j.mcn.2006.04.003</identifier><identifier>PMID: 16730456</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; Astrocytes - cytology ; Astrocytes - physiology ; Biomarkers - metabolism ; Brain Tissue Transplantation - methods ; Cell Differentiation - physiology ; Cell Lineage - physiology ; Cell Separation ; Cell Survival - physiology ; Glutamate Decarboxylase - metabolism ; Interneurons - cytology ; Interneurons - metabolism ; Isoenzymes - metabolism ; Lateral Ventricles - cytology ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microtubule-Associated Proteins - metabolism ; Neural Cell Adhesion Molecule L1 - metabolism ; Neuroglia - cytology ; Neuroglia - physiology ; Neurons - cytology ; Neurons - physiology ; Neuropeptides - metabolism ; Oligodendroglia - cytology ; Oligodendroglia - physiology ; Sialic Acids - metabolism ; Stem Cell Transplantation ; Stem Cells - cytology ; Stem Cells - physiology ; Tubulin - metabolism</subject><ispartof>Molecular and cellular neuroscience, 2006-05, Vol.32 (1), p.187-198</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-e76119f6508b88c247d12074bd9a651bec04f841592d8fb68ba39b21c63885243</citedby><cites>FETCH-LOGICAL-c382t-e76119f6508b88c247d12074bd9a651bec04f841592d8fb68ba39b21c63885243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1044743106000753$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16730456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seidenfaden, Ralph</creatorcontrib><creatorcontrib>Desoeuvre, Angélique</creatorcontrib><creatorcontrib>Bosio, Andreas</creatorcontrib><creatorcontrib>Virard, Isabelle</creatorcontrib><creatorcontrib>Cremer, Harold</creatorcontrib><title>Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5
+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - physiology</subject><subject>Biomarkers - metabolism</subject><subject>Brain Tissue Transplantation - methods</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage - physiology</subject><subject>Cell Separation</subject><subject>Cell Survival - physiology</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Interneurons - cytology</subject><subject>Interneurons - metabolism</subject><subject>Isoenzymes - metabolism</subject><subject>Lateral Ventricles - cytology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Neural Cell Adhesion Molecule L1 - metabolism</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - physiology</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Neuropeptides - metabolism</subject><subject>Oligodendroglia - cytology</subject><subject>Oligodendroglia - physiology</subject><subject>Sialic Acids - metabolism</subject><subject>Stem Cell Transplantation</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - physiology</subject><subject>Tubulin - metabolism</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EoqXwAbggn7gljP_EscUJVbRFqtRDgQMXK3EmxavEXmxnJb49Xu1K3OA0bzS_eYf3CHnLoGXA1Iddu7rQcgDVgmwBxDNyycB0jRG8f37UUja9FOyCvMp5BwAdN-IluWCqFyA7dUn87eKHhboYDpiyj4HGmT5-_9FMmPwBp3pZV19KVQG3FEOF9wndlnJMmQ5zwUTRlbj3jj6luvvwRH0okZafSIdpWwod0-DDa_JiHpaMb87ziny7-fz1-q65f7j9cv3pvnFC89Jgrxgzs-pAj1o7LvuJcejlOJlBdWxEB3LWknWGT3oelR4HYUbOnBJad1yKK_L-5LtP8deGudjVZ4fLMgSMW7ZKgwHD1X9B1nMlJfQVZCfQpZhzwtnuk1-H9NsysMci7M7WIuyxCAvS1iLqz7uz-TauOP39OCdfgY8nAGsWB4_JZucxOJx8jbfYKfp_2P8BzcGZIQ</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Seidenfaden, Ralph</creator><creator>Desoeuvre, Angélique</creator><creator>Bosio, Andreas</creator><creator>Virard, Isabelle</creator><creator>Cremer, Harold</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain</title><author>Seidenfaden, Ralph ; Desoeuvre, Angélique ; Bosio, Andreas ; Virard, Isabelle ; Cremer, Harold</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-e76119f6508b88c247d12074bd9a651bec04f841592d8fb68ba39b21c63885243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - physiology</topic><topic>Biomarkers - metabolism</topic><topic>Brain Tissue Transplantation - methods</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Lineage - physiology</topic><topic>Cell Separation</topic><topic>Cell Survival - physiology</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Interneurons - cytology</topic><topic>Interneurons - metabolism</topic><topic>Isoenzymes - metabolism</topic><topic>Lateral Ventricles - cytology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Neural Cell Adhesion Molecule L1 - metabolism</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - physiology</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Neuropeptides - metabolism</topic><topic>Oligodendroglia - cytology</topic><topic>Oligodendroglia - physiology</topic><topic>Sialic Acids - metabolism</topic><topic>Stem Cell Transplantation</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - physiology</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seidenfaden, Ralph</creatorcontrib><creatorcontrib>Desoeuvre, Angélique</creatorcontrib><creatorcontrib>Bosio, Andreas</creatorcontrib><creatorcontrib>Virard, Isabelle</creatorcontrib><creatorcontrib>Cremer, Harold</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seidenfaden, Ralph</au><au>Desoeuvre, Angélique</au><au>Bosio, Andreas</au><au>Virard, Isabelle</au><au>Cremer, Harold</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>32</volume><issue>1</issue><spage>187</spage><epage>198</epage><pages>187-198</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>In the adult mouse forebrain, large numbers of neuronal precursors, destined to become GABA- and dopamine-producing interneurons of the olfactory bulb (OB), are generated in the subventricular zone (SVZ). Although this neurogenic system represents a potential reservoir of stem and progenitor cells for brain repair approaches, information about the survival and differentiation of SVZ-derived cells in ectopic brain regions is still fragmentary. We show here that ectopic grafting of SVZ tissue gave rise to two morphologically distinguishable cell types displaying oligodendrocytic or astrocytic characteristics. Since SVZ tissue contains neuronal and glial progenitors, we used magnetic cell sorting to deplete A2B5
+ glial progenitors from the dissociated SVZ and to positively select cells that express PSA-NCAM. This procedure allowed the purification of neuronal precursors expressing TUJ1, DCX and GAD65/67. Transplantation of these cells led again to the generation of the same two glial cell types, showing that committed interneuron precursors undergo glial differentiation outside their normal environment.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16730456</pmid><doi>10.1016/j.mcn.2006.04.003</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1044-7431 |
| ispartof | Molecular and cellular neuroscience, 2006-05, Vol.32 (1), p.187-198 |
| issn | 1044-7431 1095-9327 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68090926 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn Astrocytes - cytology Astrocytes - physiology Biomarkers - metabolism Brain Tissue Transplantation - methods Cell Differentiation - physiology Cell Lineage - physiology Cell Separation Cell Survival - physiology Glutamate Decarboxylase - metabolism Interneurons - cytology Interneurons - metabolism Isoenzymes - metabolism Lateral Ventricles - cytology Mice Mice, Inbred C57BL Mice, Transgenic Microtubule-Associated Proteins - metabolism Neural Cell Adhesion Molecule L1 - metabolism Neuroglia - cytology Neuroglia - physiology Neurons - cytology Neurons - physiology Neuropeptides - metabolism Oligodendroglia - cytology Oligodendroglia - physiology Sialic Acids - metabolism Stem Cell Transplantation Stem Cells - cytology Stem Cells - physiology Tubulin - metabolism |
| title | Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A25%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glial%20conversion%20of%20SVZ-derived%20committed%20neuronal%20precursors%20after%20ectopic%20grafting%20into%20the%20adult%20brain&rft.jtitle=Molecular%20and%20cellular%20neuroscience&rft.au=Seidenfaden,%20Ralph&rft.date=2006-05-01&rft.volume=32&rft.issue=1&rft.spage=187&rft.epage=198&rft.pages=187-198&rft.issn=1044-7431&rft.eissn=1095-9327&rft_id=info:doi/10.1016/j.mcn.2006.04.003&rft_dat=%3Cproquest_cross%3E17264407%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17264407&rft_id=info:pmid/16730456&rft_els_id=S1044743106000753&rfr_iscdi=true |