The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro
Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2005-08, Vol.45 (2), p.183-189 |
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| creator | van Nuenen, Marleen H.M.C. de Ligt, Rianne A.F. Doornbos, Robert P. van der Woude, Janneke C.J. Kuipers, Ernst J. Venema, Koen |
| description | Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate,
iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and
iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired. |
| doi_str_mv | 10.1016/j.femsim.2005.03.010 |
| format | Article |
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iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and
iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1016/j.femsim.2005.03.010</identifier><identifier>PMID: 15939578</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Ammonium ; Ammonium Chloride - metabolism ; Ammonium Chloride - pharmacology ; Biological and medical sciences ; Butyrates - metabolism ; Butyrates - pharmacology ; Caco-2 Cells ; Colon ; Cytokines ; Epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - immunology ; Epithelial Cells - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Immune response ; Immunity, Mucosal - drug effects ; In Vitro Techniques ; Inflammatory Bowel Diseases - etiology ; Inflammatory Bowel Diseases - immunology ; Inflammatory Bowel Diseases - microbiology ; Interleukin 10 ; Interleukin-10 - secretion ; Intestinal epithelial cells ; Intestine ; Intestines - drug effects ; Intestines - immunology ; Intestines - microbiology ; Macrophages ; Macrophages - drug effects ; Macrophages - immunology ; Macrophages - microbiology ; Metabolites ; Microbial metabolites ; Microbiology ; Microorganisms ; Mucosal immunity ; Pentanoic Acids - metabolism ; Pentanoic Acids - pharmacology ; Tumor Necrosis Factor-alpha - secretion ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Tumors ; Tumour necrosis factor-α ; U937 Cells</subject><ispartof>FEMS immunology and medical microbiology, 2005-08, Vol.45 (2), p.183-189</ispartof><rights>2005 Federation of European Microbiological Societies</rights><rights>2005 Federation of European Microbiological Societies. 2005</rights><rights>2005 INIST-CNRS</rights><rights>2005 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5843-3050c869a5019697bb490ae19380672e501e63c89f3cd44e6573a584309172963</citedby><cites>FETCH-LOGICAL-c5843-3050c869a5019697bb490ae19380672e501e63c89f3cd44e6573a584309172963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.femsim.2005.03.010$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.femsim.2005.03.010$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17006727$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15939578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Nuenen, Marleen H.M.C.</creatorcontrib><creatorcontrib>de Ligt, Rianne A.F.</creatorcontrib><creatorcontrib>Doornbos, Robert P.</creatorcontrib><creatorcontrib>van der Woude, Janneke C.J.</creatorcontrib><creatorcontrib>Kuipers, Ernst J.</creatorcontrib><creatorcontrib>Venema, Koen</creatorcontrib><title>The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate,
iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and
iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.</description><subject>Ammonium</subject><subject>Ammonium Chloride - metabolism</subject><subject>Ammonium Chloride - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Butyrates - metabolism</subject><subject>Butyrates - pharmacology</subject><subject>Caco-2 Cells</subject><subject>Colon</subject><subject>Cytokines</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - immunology</subject><subject>Epithelial Cells - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunity, Mucosal - drug effects</subject><subject>In Vitro Techniques</subject><subject>Inflammatory Bowel Diseases - etiology</subject><subject>Inflammatory Bowel Diseases - immunology</subject><subject>Inflammatory Bowel Diseases - microbiology</subject><subject>Interleukin 10</subject><subject>Interleukin-10 - secretion</subject><subject>Intestinal epithelial cells</subject><subject>Intestine</subject><subject>Intestines - drug effects</subject><subject>Intestines - immunology</subject><subject>Intestines - microbiology</subject><subject>Macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Macrophages - microbiology</subject><subject>Metabolites</subject><subject>Microbial metabolites</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mucosal immunity</subject><subject>Pentanoic Acids - metabolism</subject><subject>Pentanoic Acids - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - secretion</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Tumors</subject><subject>Tumour necrosis factor-α</subject><subject>U937 Cells</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNklFr1TAUx4Mo7jr9BiIF0bd2J03SJC-CDKeDiS8TfAtpeurNpW2uSTvZtzelFwY-6J5Ckt_v5CT_EPKaQkWBNheHqscx-bGqAUQFrAIKT8iOCsnLRosfT8kOdK1KVXN-Rl6kdAAArgGekzMqNNNCqh3pbvdY-KkfFpwcFqEvRu9iaL0dihFn24bBz5iKMBX7ZbRTZvN09lPex6Of9zisqMNhSIWdumK0WT_u7c8s-am483MML8mz3g4JX53Gc_L96tPt5Zfy5tvn68uPN6UTirOSgQCnGm0FUN1o2ba5XYtUMwWNrDEvY8Oc0j1zHefYCMnsaoKmstYNOyfvt7rHGH4tuU0z-rS2ZicMSzKNAg1Cqf-CVDLBKfAMvv0LPIQl5ssnUzOQVAgtV4pvVL56ShF7c4x-tPHeUDBrWOZgtrDMGpYBZnJYWXtzKr60I3YP0imdDLw7ATY5O_TRTs6nB07C-i4yc2rjfvsB7x91uLm6_koVy-rFpobl-NiuP2wG5iTvPEaTnF8_T-cjutl0wf-7wB_WS9OK</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>van Nuenen, Marleen H.M.C.</creator><creator>de Ligt, Rianne A.F.</creator><creator>Doornbos, Robert P.</creator><creator>van der Woude, Janneke C.J.</creator><creator>Kuipers, Ernst J.</creator><creator>Venema, Koen</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro</title><author>van Nuenen, Marleen H.M.C. ; de Ligt, Rianne A.F. ; Doornbos, Robert P. ; van der Woude, Janneke C.J. ; Kuipers, Ernst J. ; Venema, Koen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5843-3050c869a5019697bb490ae19380672e501e63c89f3cd44e6573a584309172963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Ammonium</topic><topic>Ammonium Chloride - metabolism</topic><topic>Ammonium Chloride - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Butyrates - metabolism</topic><topic>Butyrates - pharmacology</topic><topic>Caco-2 Cells</topic><topic>Colon</topic><topic>Cytokines</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - immunology</topic><topic>Epithelial Cells - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immunity, Mucosal - drug effects</topic><topic>In Vitro Techniques</topic><topic>Inflammatory Bowel Diseases - etiology</topic><topic>Inflammatory Bowel Diseases - immunology</topic><topic>Inflammatory Bowel Diseases - microbiology</topic><topic>Interleukin 10</topic><topic>Interleukin-10 - secretion</topic><topic>Intestinal epithelial cells</topic><topic>Intestine</topic><topic>Intestines - drug effects</topic><topic>Intestines - immunology</topic><topic>Intestines - microbiology</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Macrophages - microbiology</topic><topic>Metabolites</topic><topic>Microbial metabolites</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Mucosal immunity</topic><topic>Pentanoic Acids - metabolism</topic><topic>Pentanoic Acids - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - secretion</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Tumors</topic><topic>Tumour necrosis factor-α</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Nuenen, Marleen H.M.C.</creatorcontrib><creatorcontrib>de Ligt, Rianne A.F.</creatorcontrib><creatorcontrib>Doornbos, Robert P.</creatorcontrib><creatorcontrib>van der Woude, Janneke C.J.</creatorcontrib><creatorcontrib>Kuipers, Ernst J.</creatorcontrib><creatorcontrib>Venema, Koen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Nuenen, Marleen H.M.C.</au><au>de Ligt, Rianne A.F.</au><au>Doornbos, Robert P.</au><au>van der Woude, Janneke C.J.</au><au>Kuipers, Ernst J.</au><au>Venema, Koen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2005-08</date><risdate>2005</risdate><volume>45</volume><issue>2</issue><spage>183</spage><epage>189</epage><pages>183-189</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate,
iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and
iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>15939578</pmid><doi>10.1016/j.femsim.2005.03.010</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0928-8244 |
| ispartof | FEMS immunology and medical microbiology, 2005-08, Vol.45 (2), p.183-189 |
| issn | 0928-8244 1574-695X 2049-632X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68090588 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Ammonium Ammonium Chloride - metabolism Ammonium Chloride - pharmacology Biological and medical sciences Butyrates - metabolism Butyrates - pharmacology Caco-2 Cells Colon Cytokines Epithelial cells Epithelial Cells - drug effects Epithelial Cells - immunology Epithelial Cells - microbiology Fundamental and applied biological sciences. Psychology Humans Immune response Immunity, Mucosal - drug effects In Vitro Techniques Inflammatory Bowel Diseases - etiology Inflammatory Bowel Diseases - immunology Inflammatory Bowel Diseases - microbiology Interleukin 10 Interleukin-10 - secretion Intestinal epithelial cells Intestine Intestines - drug effects Intestines - immunology Intestines - microbiology Macrophages Macrophages - drug effects Macrophages - immunology Macrophages - microbiology Metabolites Microbial metabolites Microbiology Microorganisms Mucosal immunity Pentanoic Acids - metabolism Pentanoic Acids - pharmacology Tumor Necrosis Factor-alpha - secretion Tumor necrosis factor-TNF Tumor necrosis factor-α Tumors Tumour necrosis factor-α U937 Cells |
| title | The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro |
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