Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs
The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal. To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and trea...
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| Veröffentlicht in: | Spine (Philadelphia, Pa. 1976) Pa. 1976), 2006-06, Vol.31 (14), p.1547-1554 |
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| container_title | Spine (Philadelphia, Pa. 1976) |
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| creator | ANTONIOU, John MWALE, Fackson DEMERS, Caroline N BEAUDOIN, Gilles GOSWAMI, Tapas A, Max ALINI, Mauro |
| description | The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal.
To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration.
Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal.
The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 degrees C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters.
Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment.
Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR. |
| doi_str_mv | 10.1097/01.brs.0000221995.77177.9d |
| format | Article |
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To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration.
Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal.
The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 degrees C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters.
Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment.
Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.</description><identifier>ISSN: 0362-2436</identifier><identifier>EISSN: 1528-1159</identifier><identifier>DOI: 10.1097/01.brs.0000221995.77177.9d</identifier><identifier>PMID: 16778686</identifier><identifier>CODEN: SPINDD</identifier><language>eng</language><publisher>Philadelphia, PA: Lippincott</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cerebrospinal fluid. Meninges. Spinal cord ; Collagen - metabolism ; Collagenases - pharmacology ; Drug toxicity and drugs side effects treatment ; Hyaluronic Acid - metabolism ; Hyaluronoglucosaminidase - drug effects ; Intervertebral Disc - drug effects ; Intervertebral Disc - metabolism ; Intervertebral Disc - pathology ; Intervertebral Disc Chemolysis ; Investigative techniques, diagnostic techniques (general aspects) ; Magnetic Resonance Imaging ; Medical sciences ; Nervous system ; Nervous system (semeiology, syndromes) ; Neurology ; Pharmacology. Drug treatments ; Protein Denaturation ; Proteoglycans - metabolism ; Radiodiagnosis. Nmr imagery. Nmr spectrometry ; Toxicity: nervous system and muscle ; Trypsin - pharmacology</subject><ispartof>Spine (Philadelphia, Pa. 1976), 2006-06, Vol.31 (14), p.1547-1554</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-94ee14b22ec37002865aabe69e30c0108a769b898bf400efcd1a42533d45739a3</citedby><cites>FETCH-LOGICAL-c376t-94ee14b22ec37002865aabe69e30c0108a769b898bf400efcd1a42533d45739a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17884736$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16778686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ANTONIOU, John</creatorcontrib><creatorcontrib>MWALE, Fackson</creatorcontrib><creatorcontrib>DEMERS, Caroline N</creatorcontrib><creatorcontrib>BEAUDOIN, Gilles</creatorcontrib><creatorcontrib>GOSWAMI, Tapas</creatorcontrib><creatorcontrib>A, Max</creatorcontrib><creatorcontrib>ALINI, Mauro</creatorcontrib><title>Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs</title><title>Spine (Philadelphia, Pa. 1976)</title><addtitle>Spine (Phila Pa 1976)</addtitle><description>The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal.
To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration.
Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal.
The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 degrees C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters.
Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment.
Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cerebrospinal fluid. Meninges. Spinal cord</subject><subject>Collagen - metabolism</subject><subject>Collagenases - pharmacology</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Hyaluronic Acid - metabolism</subject><subject>Hyaluronoglucosaminidase - drug effects</subject><subject>Intervertebral Disc - drug effects</subject><subject>Intervertebral Disc - metabolism</subject><subject>Intervertebral Disc - pathology</subject><subject>Intervertebral Disc Chemolysis</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Magnetic Resonance Imaging</subject><subject>Medical sciences</subject><subject>Nervous system</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Denaturation</subject><subject>Proteoglycans - metabolism</subject><subject>Radiodiagnosis. Nmr imagery. Nmr spectrometry</subject><subject>Toxicity: nervous system and muscle</subject><subject>Trypsin - pharmacology</subject><issn>0362-2436</issn><issn>1528-1159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFuLFDEQRoMo7rj6FyQI-tZtLt25-CaLN1gQQZ9DOqkeIz3pMUkvjPjjrXUHJi-VfJxKUYeQV5z1nFn9lvF-KrVneITg1o691lzr3sZHZMdHYTrOR_uY7JhUohODVFfkWa2_kFeS26fkiiutjTJqR_5-23xuqfmW7oAe_D5DS4EWqGv2OQBNmKW8p-tMIf85HRAMfllONOW4BYg0wr74iPGa76H2E2jewgJbpcdtOa4VL5in3KDcQWkwFb_QmGqoz8mT2S8VXpzrNfnx8cP3m8_d7ddPX27e33ZBatU6OwDwYRIC8I07GzV6P4GyIFlgnBmvlZ2MNdM8MAZziNwPYpQyDqOW1str8ubh32NZf29QmzvgeFgWn2HdqlOGGS2ZQPDdAxjKWmuB2R0LCignx5m7d-8Yd-jeXdy7_-6djdj88jxlmw4QL61n2Qi8PgO-osO5oOBUL5w2ZtBSyX9iCJFt</recordid><startdate>20060615</startdate><enddate>20060615</enddate><creator>ANTONIOU, John</creator><creator>MWALE, Fackson</creator><creator>DEMERS, Caroline N</creator><creator>BEAUDOIN, Gilles</creator><creator>GOSWAMI, Tapas</creator><creator>A, Max</creator><creator>ALINI, Mauro</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060615</creationdate><title>Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs</title><author>ANTONIOU, John ; MWALE, Fackson ; DEMERS, Caroline N ; BEAUDOIN, Gilles ; GOSWAMI, Tapas ; A, Max ; ALINI, Mauro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-94ee14b22ec37002865aabe69e30c0108a769b898bf400efcd1a42533d45739a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cerebrospinal fluid. Meninges. Spinal cord</topic><topic>Collagen - metabolism</topic><topic>Collagenases - pharmacology</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Hyaluronic Acid - metabolism</topic><topic>Hyaluronoglucosaminidase - drug effects</topic><topic>Intervertebral Disc - drug effects</topic><topic>Intervertebral Disc - metabolism</topic><topic>Intervertebral Disc - pathology</topic><topic>Intervertebral Disc Chemolysis</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Magnetic Resonance Imaging</topic><topic>Medical sciences</topic><topic>Nervous system</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Denaturation</topic><topic>Proteoglycans - metabolism</topic><topic>Radiodiagnosis. Nmr imagery. Nmr spectrometry</topic><topic>Toxicity: nervous system and muscle</topic><topic>Trypsin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ANTONIOU, John</creatorcontrib><creatorcontrib>MWALE, Fackson</creatorcontrib><creatorcontrib>DEMERS, Caroline N</creatorcontrib><creatorcontrib>BEAUDOIN, Gilles</creatorcontrib><creatorcontrib>GOSWAMI, Tapas</creatorcontrib><creatorcontrib>A, Max</creatorcontrib><creatorcontrib>ALINI, Mauro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ANTONIOU, John</au><au>MWALE, Fackson</au><au>DEMERS, Caroline N</au><au>BEAUDOIN, Gilles</au><au>GOSWAMI, Tapas</au><au>A, Max</au><au>ALINI, Mauro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs</atitle><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle><addtitle>Spine (Phila Pa 1976)</addtitle><date>2006-06-15</date><risdate>2006</risdate><volume>31</volume><issue>14</issue><spage>1547</spage><epage>1554</epage><pages>1547-1554</pages><issn>0362-2436</issn><eissn>1528-1159</eissn><coden>SPINDD</coden><abstract>The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal.
To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration.
Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal.
The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 degrees C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters.
Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment.
Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>16778686</pmid><doi>10.1097/01.brs.0000221995.77177.9d</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Biological and medical sciences Cattle Cerebrospinal fluid. Meninges. Spinal cord Collagen - metabolism Collagenases - pharmacology Drug toxicity and drugs side effects treatment Hyaluronic Acid - metabolism Hyaluronoglucosaminidase - drug effects Intervertebral Disc - drug effects Intervertebral Disc - metabolism Intervertebral Disc - pathology Intervertebral Disc Chemolysis Investigative techniques, diagnostic techniques (general aspects) Magnetic Resonance Imaging Medical sciences Nervous system Nervous system (semeiology, syndromes) Neurology Pharmacology. Drug treatments Protein Denaturation Proteoglycans - metabolism Radiodiagnosis. Nmr imagery. Nmr spectrometry Toxicity: nervous system and muscle Trypsin - pharmacology |
| title | Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs |
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