Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells
In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell r...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2006-06, Vol.312 (5780), p.1672-1676 |
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| creator | Qi, Hai Egen, Jackson G Huang, Alex Y.C Germain, Ronald N |
| description | In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo. |
| doi_str_mv | 10.1126/science.1125703 |
| format | Article |
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How and where within a lymph node such unprocessed antigens become available for naïve B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1125703</identifier><identifier>PMID: 16778060</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies ; Antigens ; Antigens - immunology ; B lymphocytes ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; Biological and medical sciences ; Calcium - metabolism ; Calcium Signaling ; Cell interactions ; Cell Movement ; Cell Shape ; Coculture Techniques ; Dendritic cells ; Dendritic Cells - immunology ; Endocytosis ; Follicles ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hepatitis E virus ; Human migration ; Immunity ; Immunobiology ; Immunoglobulins ; Immunology ; Lymph nodes ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Lymphatic system ; Lymphocyte Activation ; Lymphocytes ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Muramidase - immunology ; Receptors, Antigen, B-Cell - immunology ; Signal transduction ; T lymphocytes ; T-Lymphocytes - immunology</subject><ispartof>Science (American Association for the Advancement of Science), 2006-06, Vol.312 (5780), p.1672-1676</ispartof><rights>Copyright 2006 American Association for the Advancement of Science</rights><rights>2006 INIST-CNRS</rights><rights>Copyright American Association for the Advancement of Science Jun 16, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-a44195dd9a34408dd83cd5c31c299767d19e6e399bb82a2f3a7d972e920e4d533</citedby><cites>FETCH-LOGICAL-c587t-a44195dd9a34408dd83cd5c31c299767d19e6e399bb82a2f3a7d972e920e4d533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3846503$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3846503$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,2871,2872,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17929632$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16778060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qi, Hai</creatorcontrib><creatorcontrib>Egen, Jackson G</creatorcontrib><creatorcontrib>Huang, Alex Y.C</creatorcontrib><creatorcontrib>Germain, Ronald N</creatorcontrib><title>Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens - immunology</subject><subject>B lymphocytes</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling</subject><subject>Cell interactions</subject><subject>Cell Movement</subject><subject>Cell Shape</subject><subject>Coculture Techniques</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Endocytosis</subject><subject>Follicles</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hepatitis E virus</subject><subject>Human migration</subject><subject>Immunity</subject><subject>Immunobiology</subject><subject>Immunoglobulins</subject><subject>Immunology</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymphatic system</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Muramidase - immunology</subject><subject>Receptors, Antigen, B-Cell - immunology</subject><subject>Signal transduction</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2PEyEYh4nRuHX17MUoMdHbuMA7fB27df1IGj2se55QYCrNdOjCjLH_vdSZuImXngh5Hx7elx8IvaTkA6VMXGUbfG_9acMlgUdoQYnmlWYEHqMFISAqRSS_QM9y3hFSahqeogsqpFREkAW6vfk9JNPGrgt27EzCSzuEX2YIscexxevj_vATf4vO42u88l2X8eaIl_0Qtr6vrr1Jod_ij753KQzBTshz9KQ1XfYv5vUS3X26-bH6Uq2_f_66Wq4ry5UcKlPXVHPntIG6Jso5BdZxC9QyraWQjmovPGi92ShmWAtGOi2ZL8P52nGAS_R-8h5SvB99Hpp9yLZ0YHofx9wIRVQtZX0WBAHAqTxvZJRyKQt5DqSyJKApK-Db_8BdHFNfnqXIgCvg9cl2NUE2xZyTb5tDCnuTjg0lzSnnZs65mXMuJ17P2nGz9-6Bn4MtwLsZMNmark2mtyE_cFIzLeDU36uJ2-Uhpn91ULXgf-95M5VbExuzTUVxd8sIBUKJKl-Nwh8qr8GD</recordid><startdate>20060616</startdate><enddate>20060616</enddate><creator>Qi, Hai</creator><creator>Egen, Jackson G</creator><creator>Huang, Alex Y.C</creator><creator>Germain, Ronald N</creator><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T5</scope><scope>7X8</scope></search><sort><creationdate>20060616</creationdate><title>Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells</title><author>Qi, Hai ; Egen, Jackson G ; Huang, Alex Y.C ; Germain, Ronald N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-a44195dd9a34408dd83cd5c31c299767d19e6e399bb82a2f3a7d972e920e4d533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antigens - immunology</topic><topic>B lymphocytes</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling</topic><topic>Cell interactions</topic><topic>Cell Movement</topic><topic>Cell Shape</topic><topic>Coculture Techniques</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Endocytosis</topic><topic>Follicles</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hepatitis E virus</topic><topic>Human migration</topic><topic>Immunity</topic><topic>Immunobiology</topic><topic>Immunoglobulins</topic><topic>Immunology</topic><topic>Lymph nodes</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymphatic system</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Muramidase - immunology</topic><topic>Receptors, Antigen, B-Cell - immunology</topic><topic>Signal transduction</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qi, Hai</creatorcontrib><creatorcontrib>Egen, Jackson G</creatorcontrib><creatorcontrib>Huang, Alex Y.C</creatorcontrib><creatorcontrib>Germain, Ronald N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qi, Hai</au><au>Egen, Jackson G</au><au>Huang, Alex Y.C</au><au>Germain, Ronald N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2006-06-16</date><risdate>2006</risdate><volume>312</volume><issue>5780</issue><spage>1672</spage><epage>1676</epage><pages>1672-1676</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>In contrast to naïve T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naïve B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>16778060</pmid><doi>10.1126/science.1125703</doi><tpages>5</tpages></addata></record> |
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| ispartof | Science (American Association for the Advancement of Science), 2006-06, Vol.312 (5780), p.1672-1676 |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies Antigens Antigens - immunology B lymphocytes B-Lymphocytes - cytology B-Lymphocytes - immunology Biological and medical sciences Calcium - metabolism Calcium Signaling Cell interactions Cell Movement Cell Shape Coculture Techniques Dendritic cells Dendritic Cells - immunology Endocytosis Follicles Fundamental and applied biological sciences. Psychology Fundamental immunology Hepatitis E virus Human migration Immunity Immunobiology Immunoglobulins Immunology Lymph nodes Lymph Nodes - cytology Lymph Nodes - immunology Lymphatic system Lymphocyte Activation Lymphocytes Mice Mice, Inbred C57BL Mice, Transgenic Muramidase - immunology Receptors, Antigen, B-Cell - immunology Signal transduction T lymphocytes T-Lymphocytes - immunology |
| title | Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells |
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