Expression in Escherichia coli, Purification and Characterization of Thermoanaerobacter tengcongensis Ribosome Recycling Factor

A very promising approach to understanding the mechanism of protein thermostability is to investigate the structure-function relationship of homologous proteins with different thermostabilities. Ribosome recycling factor (RRF), which is an essential factor for protein synthesis in bacteria, may be a...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 2005-07, Vol.138 (1), p.89-94
Hauptverfasser:	Guo, Peng, Zhang, Liqiang, Qi, Zhen, Chen, Runsheng, Jing, Guozhong
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Circular Dichroism Cloning, Molecular Escherichia coli Escherichia coli - chemistry Escherichia coli - genetics expression and characterization Genetic Complementation Test Hot Temperature Molecular Sequence Data Protein Denaturation Recombinant Proteins - chemistry Ribosomal Proteins - chemistry Ribosomal Proteins - isolation & purification ribosome recycling factor Sequence Homology, Amino Acid Thermoanaerobacter - chemistry Thermoanaerobacter - genetics Thermoanaerobacter - metabolism Thermoanaerobacter tengcongensis thermostability
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