Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells
Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (...
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| Veröffentlicht in: | Journal of animal science 2006-07, Vol.84 (7), p.1666-1681 |
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| creator | Hausman, G.J Poulos, S.P Richardson, R.L Barb, C.R Andacht, T Kirk, H.C Mynatt, R.L |
| description | Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth. |
| doi_str_mv | 10.2527/jas.2005-539 |
| format | Article |
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Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.2527/jas.2005-539</identifier><identifier>PMID: 16775050</identifier><language>eng</language><publisher>Savoy, IL: American Society of Animal Science</publisher><subject>adipocytes ; adipose tissue ; Adipose Tissue - metabolism ; animal growth ; Animal productions ; Animals ; Animals, Newborn - metabolism ; apolipoproteins ; Biological and medical sciences ; cell culture ; Cells, Cultured ; complementary DNA ; Culture Media, Conditioned ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; fetus ; Fetus - metabolism ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation, Developmental ; growth factors ; insulin-like growth factor binding proteins ; messenger RNA ; neonates ; Oligonucleotide Array Sequence Analysis ; piglets ; protein secretion ; puberty ; relaxin ; Stromal Cells - metabolism ; Swine - growth & development ; Swine - metabolism ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of animal science, 2006-07, Vol.84 (7), p.1666-1681</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17863545$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16775050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hausman, G.J</creatorcontrib><creatorcontrib>Poulos, S.P</creatorcontrib><creatorcontrib>Richardson, R.L</creatorcontrib><creatorcontrib>Barb, C.R</creatorcontrib><creatorcontrib>Andacht, T</creatorcontrib><creatorcontrib>Kirk, H.C</creatorcontrib><creatorcontrib>Mynatt, R.L</creatorcontrib><title>Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth.</description><subject>adipocytes</subject><subject>adipose tissue</subject><subject>Adipose Tissue - metabolism</subject><subject>animal growth</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Animals, Newborn - metabolism</subject><subject>apolipoproteins</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>Cells, Cultured</subject><subject>complementary DNA</subject><subject>Culture Media, Conditioned</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>fetus</subject><subject>Fetus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>growth factors</subject><subject>insulin-like growth factor binding proteins</subject><subject>messenger RNA</subject><subject>neonates</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>piglets</subject><subject>protein secretion</subject><subject>puberty</subject><subject>relaxin</subject><subject>Stromal Cells - metabolism</subject><subject>Swine - growth & development</subject><subject>Swine - metabolism</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1EAMhkcIRJeWG2fIBW5px57MR46oAopUiUPpeeRMPNupssmSSUD998y2i3rkZNl-9Pq1LcQ7kOeo0V7cUz5HKXWtVftCbECjrhUY9VJspESonQM8EW9yvpcSULf6tTgBY62WWm6Ev-Ew88J9tZ-nhdOYKxr7assj5yqNVeSFhsfSyNNIh2SfthX1aT9lrpaU88qP_bzM046G-jflsA40V4GHIZ-JV5GGzG-P8VTcfv3y8_Kqvv7x7fvl5-s6KtRL3UfbGWyj7RsIjKHREAlMB1qB6sjFDjpoEB3GEDha7LQOzLLBECM0pE7FpyfdssavlfPidykfHFDxvWZvnLTGOfdfECwaLVtTwPdHcO123Pv9nHY0P_h_tyvAxyNQNqYhzjSGlJ8564zSjX6eeJe2d3_SzD6XOw1FFnx5nmu8LaLmMPHDExhp8rSdi9jtDUpQEqSTLUr1FzoZlIs</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Hausman, G.J</creator><creator>Poulos, S.P</creator><creator>Richardson, R.L</creator><creator>Barb, C.R</creator><creator>Andacht, T</creator><creator>Kirk, H.C</creator><creator>Mynatt, R.L</creator><general>American Society of Animal Science</general><general>Am Soc Animal Sci</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells</title><author>Hausman, G.J ; Poulos, S.P ; Richardson, R.L ; Barb, C.R ; Andacht, T ; Kirk, H.C ; Mynatt, R.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f325t-df7b629f7d41ce2c451fa16b15313ba8fb1b142282fccef72b55cee042cff14a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>adipocytes</topic><topic>adipose tissue</topic><topic>Adipose Tissue - metabolism</topic><topic>animal growth</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Animals, Newborn - metabolism</topic><topic>apolipoproteins</topic><topic>Biological and medical sciences</topic><topic>cell culture</topic><topic>Cells, Cultured</topic><topic>complementary DNA</topic><topic>Culture Media, Conditioned</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>fetus</topic><topic>Fetus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>growth factors</topic><topic>insulin-like growth factor binding proteins</topic><topic>messenger RNA</topic><topic>neonates</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>piglets</topic><topic>protein secretion</topic><topic>puberty</topic><topic>relaxin</topic><topic>Stromal Cells - metabolism</topic><topic>Swine - growth & development</topic><topic>Swine - metabolism</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hausman, G.J</creatorcontrib><creatorcontrib>Poulos, S.P</creatorcontrib><creatorcontrib>Richardson, R.L</creatorcontrib><creatorcontrib>Barb, C.R</creatorcontrib><creatorcontrib>Andacht, T</creatorcontrib><creatorcontrib>Kirk, H.C</creatorcontrib><creatorcontrib>Mynatt, R.L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hausman, G.J</au><au>Poulos, S.P</au><au>Richardson, R.L</au><au>Barb, C.R</au><au>Andacht, T</au><au>Kirk, H.C</au><au>Mynatt, R.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>84</volume><issue>7</issue><spage>1666</spage><epage>1681</epage><pages>1666-1681</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>Although microarray and proteomic studies have indicated the expression of unique and unexpected genes and their products in human and rodent adipose tissue, similar studies of meat animal adipose tissue have not been reported. Thus, total RNA was isolated from stromal-vascular (S-V) cell cultures (n = 4; 2 arrays; 2 cultures/array) from 90-d (79% of gestation) fetuses and adipose tissue from 105-d (92% of gestation) fetuses (n = 2) and neonatal (5-d-old) pigs (n = 2). Duplicate adipose tissue microarrays (n = 4) represented RNA samples from a pig and a fetus. Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing more than 600 pig genes involved in growth and reproduction. Microarray studies showed significant expression of 40 genes encoding for known adipose tissue secreted proteins in fetal S-V cell cultures and adipose tissue. Expression of 10 genes encoding secreted proteins not known to be expressed by adipose tissue was also observed in neonatal adipose tissue and fetal S-V cell cultures. Additionally, the agouti gene was detected by reverse transcription-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and fetal and young pig S-V cell culture-conditioned media identified multiple secreted proteins including heparin-like epidermal growth factor-like growth factor and several apolipoproteins. Another adipose tissue secreted protein, plasminogen activator inhibitor-1, was identified by ELISA in S-V cell culture media. A group of 20 adipose tissue secreted proteins were detected or identified using the gene microarray and the proteomic and protein assay approaches including apolipoprotein-A1, apolipoprotein-E, relaxin, brain-derived neurotrophic factor, and IGF binding protein-5. These studies demonstrate, for the first time, the expression of several major secreted proteins in pig adipose tissue that may influence local and central metabolism and growth.</abstract><cop>Savoy, IL</cop><pub>American Society of Animal Science</pub><pmid>16775050</pmid><doi>10.2527/jas.2005-539</doi><tpages>16</tpages></addata></record> |
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| subjects | adipocytes adipose tissue Adipose Tissue - metabolism animal growth Animal productions Animals Animals, Newborn - metabolism apolipoproteins Biological and medical sciences cell culture Cells, Cultured complementary DNA Culture Media, Conditioned Endothelium, Vascular - cytology Endothelium, Vascular - metabolism fetus Fetus - metabolism Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Developmental growth factors insulin-like growth factor binding proteins messenger RNA neonates Oligonucleotide Array Sequence Analysis piglets protein secretion puberty relaxin Stromal Cells - metabolism Swine - growth & development Swine - metabolism Terrestrial animal productions Vertebrates |
| title | Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells |
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