Localization and ontogeny of aquaporin-1 and -4 expression in iris and ciliary epithelial cells in rats
The precise localization of aquaporin (AQP)1 and AQP4 was studied in iris and ciliary epithelial cells, in both mature and developing rats, to elucidate the molecular mechanisms underlying aqueous humor balance. Anterior segments of eyes dissected from embryonic day (E)13, E15, E18, and E20, postnat...
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| Veröffentlicht in: | Cell and tissue research 2006-07, Vol.325 (1), p.101-109 |
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| description | The precise localization of aquaporin (AQP)1 and AQP4 was studied in iris and ciliary epithelial cells, in both mature and developing rats, to elucidate the molecular mechanisms underlying aqueous humor balance. Anterior segments of eyes dissected from embryonic day (E)13, E15, E18, and E20, postnatal day (P)0, P7, and P14, and postnatal week 8 rats were subjected to immunofluorescence analysis with AQP isoform-specific antibodies. In adult rat eye, AQP1 was localized to the apical and basolateral plasma membranes of iris epithelial cell layers and of anterior ciliary non-pigmented epithelial (NPE) cells. Conversely, AQP4 was localized to the basolateral plasma membrane of NPE cells in ciliary epithelium and the posterior iris. Developmentally, AQP1 was detected as early as E15 in immature iris and ciliary epithelial cells, and expression persisted throughout development up to adulthood. In contrast, AQP4 was first observed at P7 in the developing pars plicata, and the AQP4-positive area gradually spread to cover the entire pars plicata as development proceeded. These findings indicate that both AQP1 and AQP4 contribute to aqueous humor secretion in the rat eye, thereby maintaining proper intraocular pressure. Moreover, AQP appears to play a major role in aqueous humor secretion in early eye development. This study thus provides a basis for understanding the molecular mechanisms of aqueous humor secretion in pathological and physiological conditions. |
| doi_str_mv | 10.1007/s00441-005-0122-z |
| format | Article |
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Anterior segments of eyes dissected from embryonic day (E)13, E15, E18, and E20, postnatal day (P)0, P7, and P14, and postnatal week 8 rats were subjected to immunofluorescence analysis with AQP isoform-specific antibodies. In adult rat eye, AQP1 was localized to the apical and basolateral plasma membranes of iris epithelial cell layers and of anterior ciliary non-pigmented epithelial (NPE) cells. Conversely, AQP4 was localized to the basolateral plasma membrane of NPE cells in ciliary epithelium and the posterior iris. Developmentally, AQP1 was detected as early as E15 in immature iris and ciliary epithelial cells, and expression persisted throughout development up to adulthood. In contrast, AQP4 was first observed at P7 in the developing pars plicata, and the AQP4-positive area gradually spread to cover the entire pars plicata as development proceeded. These findings indicate that both AQP1 and AQP4 contribute to aqueous humor secretion in the rat eye, thereby maintaining proper intraocular pressure. Moreover, AQP appears to play a major role in aqueous humor secretion in early eye development. This study thus provides a basis for understanding the molecular mechanisms of aqueous humor secretion in pathological and physiological conditions.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-005-0122-z</identifier><identifier>PMID: 16525835</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Aging ; Animals ; Aquaporin 1 - metabolism ; Aquaporin 4 - metabolism ; Ciliary Body - cytology ; Ciliary Body - embryology ; Ciliary Body - metabolism ; Ciliary Body - physiology ; Epithelial Cells - metabolism ; Epithelial Cells - physiology ; Female ; Fluorescein-5-isothiocyanate ; Fluorescent Antibody Technique, Indirect ; Fluorescent Dyes ; Immunohistochemistry ; Iris - cytology ; Iris - embryology ; Iris - metabolism ; Iris - physiology ; Pregnancy ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Cell and tissue research, 2006-07, Vol.325 (1), p.101-109</ispartof><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-edc8249e1139bc6809a93ea8fc57028ffb73651fbc60e2e7b52ef571e4dd17b23</citedby><cites>FETCH-LOGICAL-c392t-edc8249e1139bc6809a93ea8fc57028ffb73651fbc60e2e7b52ef571e4dd17b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16525835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamaguchi, Yasuko</creatorcontrib><creatorcontrib>Watanabe, Takashi</creatorcontrib><creatorcontrib>Hirakata, Akito</creatorcontrib><creatorcontrib>Hida, Tetsuo</creatorcontrib><title>Localization and ontogeny of aquaporin-1 and -4 expression in iris and ciliary epithelial cells in rats</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>The precise localization of aquaporin (AQP)1 and AQP4 was studied in iris and ciliary epithelial cells, in both mature and developing rats, to elucidate the molecular mechanisms underlying aqueous humor balance. Anterior segments of eyes dissected from embryonic day (E)13, E15, E18, and E20, postnatal day (P)0, P7, and P14, and postnatal week 8 rats were subjected to immunofluorescence analysis with AQP isoform-specific antibodies. In adult rat eye, AQP1 was localized to the apical and basolateral plasma membranes of iris epithelial cell layers and of anterior ciliary non-pigmented epithelial (NPE) cells. Conversely, AQP4 was localized to the basolateral plasma membrane of NPE cells in ciliary epithelium and the posterior iris. Developmentally, AQP1 was detected as early as E15 in immature iris and ciliary epithelial cells, and expression persisted throughout development up to adulthood. In contrast, AQP4 was first observed at P7 in the developing pars plicata, and the AQP4-positive area gradually spread to cover the entire pars plicata as development proceeded. These findings indicate that both AQP1 and AQP4 contribute to aqueous humor secretion in the rat eye, thereby maintaining proper intraocular pressure. Moreover, AQP appears to play a major role in aqueous humor secretion in early eye development. This study thus provides a basis for understanding the molecular mechanisms of aqueous humor secretion in pathological and physiological conditions.</description><subject>Aging</subject><subject>Animals</subject><subject>Aquaporin 1 - metabolism</subject><subject>Aquaporin 4 - metabolism</subject><subject>Ciliary Body - cytology</subject><subject>Ciliary Body - embryology</subject><subject>Ciliary Body - metabolism</subject><subject>Ciliary Body - physiology</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - physiology</subject><subject>Female</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fluorescent Dyes</subject><subject>Immunohistochemistry</subject><subject>Iris - cytology</subject><subject>Iris - embryology</subject><subject>Iris - metabolism</subject><subject>Iris - physiology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkU9r3DAQxUVJabZJP0AuweTQm9rRf_tYlqQtLPTSQG5CtsdbBa_llWxo9tNH3l0IBAQjeL95zMwj5IbBNwZgvicAKRkFUBQY5_TwgayYFJxCacoLsgIBnBqtny7J55SeAZjUuvpELplWXJVCrch2ExrX-4ObfBgKN7RFGKawxeGlCF3h9rMbQ_QDZUeNygL_jxFTWmifX_TpqDS-9y6-FDj66R_mf1802PdpgaKb0jX52Lk-4ZdzvSKPD_d_17_o5s_P3-sfG9qIik8U26bkskLGRFU3uoTKVQJd2TXKAC-7rjZCK9ZlDZCjqRXHThmGsm2Zqbm4Il9PvmMM-xnTZHc-LZO4AcOcbLY02miVwbt34HOY45Bns5wJo6RWMkPsBDUxpBSxs2P0u7ynZWCXCOwpApsjsEsE9pB7bs_Gc73D9q3jfHPxCrSggjA</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Yamaguchi, Yasuko</creator><creator>Watanabe, Takashi</creator><creator>Hirakata, Akito</creator><creator>Hida, Tetsuo</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Localization and ontogeny of aquaporin-1 and -4 expression in iris and ciliary epithelial cells in rats</title><author>Yamaguchi, Yasuko ; Watanabe, Takashi ; Hirakata, Akito ; Hida, Tetsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-edc8249e1139bc6809a93ea8fc57028ffb73651fbc60e2e7b52ef571e4dd17b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Aquaporin 1 - metabolism</topic><topic>Aquaporin 4 - metabolism</topic><topic>Ciliary Body - cytology</topic><topic>Ciliary Body - embryology</topic><topic>Ciliary Body - metabolism</topic><topic>Ciliary Body - physiology</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - physiology</topic><topic>Female</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fluorescent Dyes</topic><topic>Immunohistochemistry</topic><topic>Iris - cytology</topic><topic>Iris - embryology</topic><topic>Iris - metabolism</topic><topic>Iris - physiology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamaguchi, Yasuko</creatorcontrib><creatorcontrib>Watanabe, Takashi</creatorcontrib><creatorcontrib>Hirakata, Akito</creatorcontrib><creatorcontrib>Hida, Tetsuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamaguchi, Yasuko</au><au>Watanabe, Takashi</au><au>Hirakata, Akito</au><au>Hida, Tetsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization and ontogeny of aquaporin-1 and -4 expression in iris and ciliary epithelial cells in rats</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>325</volume><issue>1</issue><spage>101</spage><epage>109</epage><pages>101-109</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>The precise localization of aquaporin (AQP)1 and AQP4 was studied in iris and ciliary epithelial cells, in both mature and developing rats, to elucidate the molecular mechanisms underlying aqueous humor balance. Anterior segments of eyes dissected from embryonic day (E)13, E15, E18, and E20, postnatal day (P)0, P7, and P14, and postnatal week 8 rats were subjected to immunofluorescence analysis with AQP isoform-specific antibodies. In adult rat eye, AQP1 was localized to the apical and basolateral plasma membranes of iris epithelial cell layers and of anterior ciliary non-pigmented epithelial (NPE) cells. Conversely, AQP4 was localized to the basolateral plasma membrane of NPE cells in ciliary epithelium and the posterior iris. Developmentally, AQP1 was detected as early as E15 in immature iris and ciliary epithelial cells, and expression persisted throughout development up to adulthood. In contrast, AQP4 was first observed at P7 in the developing pars plicata, and the AQP4-positive area gradually spread to cover the entire pars plicata as development proceeded. These findings indicate that both AQP1 and AQP4 contribute to aqueous humor secretion in the rat eye, thereby maintaining proper intraocular pressure. Moreover, AQP appears to play a major role in aqueous humor secretion in early eye development. This study thus provides a basis for understanding the molecular mechanisms of aqueous humor secretion in pathological and physiological conditions.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>16525835</pmid><doi>10.1007/s00441-005-0122-z</doi><tpages>9</tpages></addata></record> |
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| subjects | Aging Animals Aquaporin 1 - metabolism Aquaporin 4 - metabolism Ciliary Body - cytology Ciliary Body - embryology Ciliary Body - metabolism Ciliary Body - physiology Epithelial Cells - metabolism Epithelial Cells - physiology Female Fluorescein-5-isothiocyanate Fluorescent Antibody Technique, Indirect Fluorescent Dyes Immunohistochemistry Iris - cytology Iris - embryology Iris - metabolism Iris - physiology Pregnancy Rats Rats, Sprague-Dawley |
| title | Localization and ontogeny of aquaporin-1 and -4 expression in iris and ciliary epithelial cells in rats |
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