Phosphoproteomic Analysis with a Solid-Phase Capture-Release-Tag Approach

A comprehensive study of global phosphorylation events in biological systems is critical. We report a chemistry-based capture-release-tag method for isolation of complex phospho-Ser/Thr-containing peptides by liquid β-elimination combined with solid-phase Michael addition. The free thiol groups of 6...

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Veröffentlicht in:	Chemistry & biology 2005-07, Vol.12 (7), p.769-777
Hauptverfasser:	Tseng, Huang-Chun, Ovaa, Huib, Wei, Nancy J.C., Ploegh, Hidde, Tsai, Li-Huei
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Binding Sites Cyclin-Dependent Kinase 5 Cyclin-Dependent Kinases - metabolism Cysteamine - chemistry Isotope Labeling - methods Microtubule-Associated Proteins - analysis Microtubule-Associated Proteins - chemistry Molecular Sequence Data Phosphoproteins - analysis Phosphoproteins - metabolism Phosphorylation Phosphoserine - analysis Phosphoserine - chemistry Phosphothreonine - chemistry Phosphothreonine - metabolism Proteomics - methods tau Proteins - chemistry tau Proteins - metabolism
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