GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless
The fru4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2005-08, Vol.42 (4), p.236-246 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Dornan, Anthony J. Gailey, Donald A. Goodwin, Stephen F. |
| description | The fru4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru‐specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3‐ and P4‐derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru‐expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities. genesis 42:236–246, 2005. © 2005 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/gene.20143 |
| format | Article |
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This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru‐specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3‐ and P4‐derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru‐expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities. genesis 42:236–246, 2005. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/gene.20143</identifier><identifier>PMID: 16028231</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alleles ; Animals ; central and peripheral nervous system ; Courtship ; Drosophila ; Drosophila melanogaster - genetics ; Drosophila melanogaster - growth & development ; Drosophila Proteins - biosynthesis ; Drosophila Proteins - genetics ; Enhancer Elements, Genetic ; Female ; Fluorescent Antibody Technique ; Fushi Tarazu Transcription Factors ; GAL4-UAS system ; gene and enhancer traps ; Gene Expression Regulation, Developmental ; Gene Targeting - methods ; Genes, Insect ; Homozygote ; Male ; male courtship behavior ; Microscopy, Confocal ; muscle of Lawrence ; Mutation ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - genetics ; Sex Determination Processes ; targeted transposition ; Trans-Activators - genetics ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcription, Genetic</subject><ispartof>Genesis (New York, N.Y. : 2000), 2005-08, Vol.42 (4), p.236-246</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><rights>Copyright 2005 Wiley-Liss, Inc</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fgene.20143$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fgene.20143$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16028231$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dornan, Anthony J.</creatorcontrib><creatorcontrib>Gailey, Donald A.</creatorcontrib><creatorcontrib>Goodwin, Stephen F.</creatorcontrib><title>GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>The fru4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru‐specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3‐ and P4‐derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru‐expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities. genesis 42:236–246, 2005. © 2005 Wiley‐Liss, Inc.</description><subject>Alleles</subject><subject>Animals</subject><subject>central and peripheral nervous system</subject><subject>Courtship</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - growth & development</subject><subject>Drosophila Proteins - biosynthesis</subject><subject>Drosophila Proteins - genetics</subject><subject>Enhancer Elements, Genetic</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Fushi Tarazu Transcription Factors</subject><subject>GAL4-UAS system</subject><subject>gene and enhancer traps</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Targeting - methods</subject><subject>Genes, Insect</subject><subject>Homozygote</subject><subject>Male</subject><subject>male courtship behavior</subject><subject>Microscopy, Confocal</subject><subject>muscle of Lawrence</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Sex Determination Processes</subject><subject>targeted transposition</subject><subject>Trans-Activators - genetics</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPwzAQhC0E4n3hByCfuAV2_UpyRKUUpAIXENwsp920hjQptivg39NSHkdOO9J8syvtMHaEcIoA4mxCLZ0KQCU32C5qYbLSFE-bP1qrpx22F-MzAOhCiG22gwZEISTusrvB-VBxaqeuHVHgKbg5Ty5MKPl2wruapynxi9DFbj71jeOR3vmYEoWZb13yXctX13kdFj41FOMB26pdE-nwe-6zh8v-fe8qG94Nrnvnw8xLATKrnKnQOXQFylyWWmhNdV6OyBSqLpVEEEBK49JTBgFFYSoa6QrQQY5jJffZyXrvPHSvC4rJznwcUdO4lrpFtKaAHBTIf0HMtSykwCV4_A0uqhmN7Tz4mQsf9udXSwDXwJtv6OPPB7tqwa7-YL9asIP-bf9LLTPZOuNjovffjAsv1uQy1_bxdmBLfaPVfSltT34CdN6HQg</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Dornan, Anthony J.</creator><creator>Gailey, Donald A.</creator><creator>Goodwin, Stephen F.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless</title><author>Dornan, Anthony J. ; Gailey, Donald A. ; Goodwin, Stephen F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3203-ba6b1aa1a8137395255ef79ce684f9431020e45173946101286bec5b01a071d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>central and peripheral nervous system</topic><topic>Courtship</topic><topic>Drosophila</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila Proteins - biosynthesis</topic><topic>Drosophila Proteins - genetics</topic><topic>Enhancer Elements, Genetic</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Fushi Tarazu Transcription Factors</topic><topic>GAL4-UAS system</topic><topic>gene and enhancer traps</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Targeting - methods</topic><topic>Genes, Insect</topic><topic>Homozygote</topic><topic>Male</topic><topic>male courtship behavior</topic><topic>Microscopy, Confocal</topic><topic>muscle of Lawrence</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Sex Determination Processes</topic><topic>targeted transposition</topic><topic>Trans-Activators - genetics</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dornan, Anthony J.</creatorcontrib><creatorcontrib>Gailey, Donald A.</creatorcontrib><creatorcontrib>Goodwin, Stephen F.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dornan, Anthony J.</au><au>Gailey, Donald A.</au><au>Goodwin, Stephen F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2005-08</date><risdate>2005</risdate><volume>42</volume><issue>4</issue><spage>236</spage><epage>246</epage><pages>236-246</pages><issn>1526-954X</issn><eissn>1526-968X</eissn><abstract>The fru4 allele of the sex determination gene fruitless is induced by insertion of a P[lacZ,ry+] enhancer trap element. This insert also acts to disrupt expression of the fru P1 promoter derived male‐specific proteins, consequently impairing male courtship behavior. fru4 maps less than 2 kb upstream of the fru P3 promoter, whose function is essential for viability. We replaced this insert with a GAL4 element, P[GAL4,w+], recovering two lines with insertions in opposite orientations at the locus, one of which demonstrated fru‐specific mutant phenotypes. Reporter expression of these lines recapitulated that of P3‐ and P4‐derived proteins which, when correlated with a developmental and tissue specific survey of fru promoters' activities, uncovered a previously unsuspected complexity of fru regulation. These novel fru alleles provide the tools for manipulation of fru‐expressing cells, allowing the consequent effects to be related back to specific fru functions and the regulatory units controlling these activities. genesis 42:236–246, 2005. © 2005 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16028231</pmid><doi>10.1002/gene.20143</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alleles Animals central and peripheral nervous system Courtship Drosophila Drosophila melanogaster - genetics Drosophila melanogaster - growth & development Drosophila Proteins - biosynthesis Drosophila Proteins - genetics Enhancer Elements, Genetic Female Fluorescent Antibody Technique Fushi Tarazu Transcription Factors GAL4-UAS system gene and enhancer traps Gene Expression Regulation, Developmental Gene Targeting - methods Genes, Insect Homozygote Male male courtship behavior Microscopy, Confocal muscle of Lawrence Mutation Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins - genetics Sex Determination Processes targeted transposition Trans-Activators - genetics Transcription Factors - biosynthesis Transcription Factors - genetics Transcription, Genetic |
| title | GAL4 enhancer trap targeting of the Drosophila sex determination gene fruitless |
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