Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein
Human cytomegalovirus (HCMV) is a β-herpes virus that persists in a latent state in immunocompetent individuals. Both CD4 + and CD8 + T lymphocytes have been reported to be present at a high frequency in HCMV-seropositive individuals and are involved in the control of infection. How such frequencies...
Gespeichert in:
| Veröffentlicht in: | Viral immunology 2005-06, Vol.18 (2), p.391-396 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 396 |
|---|---|
| container_issue | 2 |
| container_start_page | 391 |
| container_title | Viral immunology |
| container_volume | 18 |
| creator | Le Roy, Emmanuelle Davignon, Jean-Luc |
| description | Human cytomegalovirus (HCMV) is a β-herpes virus that persists in a latent state in immunocompetent
individuals. Both CD4
+
and CD8
+
T lymphocytes have been reported to be present at a high
frequency in HCMV-seropositive individuals and are involved in the control of infection. How such
frequencies are maintained is not completely understood. We have observed that the canonical HLADR8
epitope of the immediate early 1 protein (IE1) contained in the IE1 (156-175) sequence shares
homologies with an IE1 sequence contained in part in the previously reported HLA-DR3 epitope,
IE1 (91-110). We thus wondered whether such homology in a single protein would translate into
recognition of the IE1 homolog sequence by HLA-DR8-restricted CD4
+
cells in addition to the
canonical epitope. We found that established HLA-DR8-restricted T cell clones are also able to
cross-recognize the IE1 (91-110) peptide, as well as a shorter 14-mer, IE1 (91-104). Moreover, the
homolog peptide IE1 (91-110) was able to generate, from a seropositive blood donor, new IE1-specific,
HLA-DR8-restricted CD4
+
T cell clones that were also cross-reactive. Those findings may
provide clues to the formation and regulation of the T-cell repertoire and memory. |
| doi_str_mv | 10.1089/vim.2005.18.391 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68065362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68065362</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-b02ed7e5d3135215e5881addb64ba881cc9df4888ad3646111ccf79dd435e3883</originalsourceid><addsrcrecordid>eNpdkU1r3DAQhkVJaTZpz70FkUMvQRvJsrTysbj5gkBDmp6FLI0TBdvaSvLC9tdXyy4p5KRheOZFMw9CXxldMqqay40flxWlYsnUkjfsA1owIVZENSt5hBZUqYqoqhbH6CSlV0qpkop_QsdMUi4awRZoczuPZsLtNocRns0QNj7Oifxag_W9t7j9UV_gJ9LCMOB2CBMk_Ag2PE_-L-A2hpTIIxib_QbwA6yzd4W4jmHE-QXw3TiC8yYDvjJx2GKGH2LI4KfP6GNvhgRfDu8p-n199dTekvufN3ft93tiuaSZdLQCtwLhOOOiYgKEUsw418m6M6W0tnF9rZQyjstaMlY6_apxruYCuFL8FH3b565j-DNDynr0yZZlzARhTloqKgWXVQHP34GvYY5T-ZuuWCNqSiUv0OUesrvFI_R6Hf1o4lYzqnc-dPGhdz40U7r4KBNnh9i5K6f4zx8EFIDsgV3bTNPgoYOY38D3gf8AjEiVyw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>219540063</pqid></control><display><type>article</type><title>Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>Le Roy, Emmanuelle ; Davignon, Jean-Luc</creator><creatorcontrib>Le Roy, Emmanuelle ; Davignon, Jean-Luc</creatorcontrib><description>Human cytomegalovirus (HCMV) is a β-herpes virus that persists in a latent state in immunocompetent
individuals. Both CD4
+
and CD8
+
T lymphocytes have been reported to be present at a high
frequency in HCMV-seropositive individuals and are involved in the control of infection. How such
frequencies are maintained is not completely understood. We have observed that the canonical HLADR8
epitope of the immediate early 1 protein (IE1) contained in the IE1 (156-175) sequence shares
homologies with an IE1 sequence contained in part in the previously reported HLA-DR3 epitope,
IE1 (91-110). We thus wondered whether such homology in a single protein would translate into
recognition of the IE1 homolog sequence by HLA-DR8-restricted CD4
+
cells in addition to the
canonical epitope. We found that established HLA-DR8-restricted T cell clones are also able to
cross-recognize the IE1 (91-110) peptide, as well as a shorter 14-mer, IE1 (91-104). Moreover, the
homolog peptide IE1 (91-110) was able to generate, from a seropositive blood donor, new IE1-specific,
HLA-DR8-restricted CD4
+
T cell clones that were also cross-reactive. Those findings may
provide clues to the formation and regulation of the T-cell repertoire and memory.</description><identifier>ISSN: 0882-8245</identifier><identifier>EISSN: 1557-8976</identifier><identifier>DOI: 10.1089/vim.2005.18.391</identifier><identifier>PMID: 16035951</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Amino Acid Sequence ; CD4-Positive T-Lymphocytes - immunology ; Clone Cells - immunology ; Cross Reactions ; Cytomegalovirus - immunology ; Cytomegalovirus - metabolism ; HLA-DR Antigens - metabolism ; HLA-DR Serological Subtypes ; Humans ; Immediate-Early Proteins - chemistry ; Immediate-Early Proteins - immunology ; Lymphocyte Activation ; Molecular Sequence Data ; Original Papers ; Peptides - chemical synthesis ; Peptides - chemistry ; Peptides - immunology ; Receptors, Interleukin-2 ; Viral Proteins - chemistry ; Viral Proteins - immunology</subject><ispartof>Viral immunology, 2005-06, Vol.18 (2), p.391-396</ispartof><rights>2005, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2005, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-b02ed7e5d3135215e5881addb64ba881cc9df4888ad3646111ccf79dd435e3883</citedby><cites>FETCH-LOGICAL-c360t-b02ed7e5d3135215e5881addb64ba881cc9df4888ad3646111ccf79dd435e3883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.liebertpub.com/doi/epdf/10.1089/vim.2005.18.391$$EPDF$$P50$$Gmaryannliebert$$H</linktopdf><linktohtml>$$Uhttps://www.liebertpub.com/doi/full/10.1089/vim.2005.18.391$$EHTML$$P50$$Gmaryannliebert$$H</linktohtml><link.rule.ids>314,780,784,3040,21722,27923,27924,55290,55302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16035951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Roy, Emmanuelle</creatorcontrib><creatorcontrib>Davignon, Jean-Luc</creatorcontrib><title>Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein</title><title>Viral immunology</title><addtitle>Viral Immunol</addtitle><description>Human cytomegalovirus (HCMV) is a β-herpes virus that persists in a latent state in immunocompetent
individuals. Both CD4
+
and CD8
+
T lymphocytes have been reported to be present at a high
frequency in HCMV-seropositive individuals and are involved in the control of infection. How such
frequencies are maintained is not completely understood. We have observed that the canonical HLADR8
epitope of the immediate early 1 protein (IE1) contained in the IE1 (156-175) sequence shares
homologies with an IE1 sequence contained in part in the previously reported HLA-DR3 epitope,
IE1 (91-110). We thus wondered whether such homology in a single protein would translate into
recognition of the IE1 homolog sequence by HLA-DR8-restricted CD4
+
cells in addition to the
canonical epitope. We found that established HLA-DR8-restricted T cell clones are also able to
cross-recognize the IE1 (91-110) peptide, as well as a shorter 14-mer, IE1 (91-104). Moreover, the
homolog peptide IE1 (91-110) was able to generate, from a seropositive blood donor, new IE1-specific,
HLA-DR8-restricted CD4
+
T cell clones that were also cross-reactive. Those findings may
provide clues to the formation and regulation of the T-cell repertoire and memory.</description><subject>Amino Acid Sequence</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>Clone Cells - immunology</subject><subject>Cross Reactions</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytomegalovirus - metabolism</subject><subject>HLA-DR Antigens - metabolism</subject><subject>HLA-DR Serological Subtypes</subject><subject>Humans</subject><subject>Immediate-Early Proteins - chemistry</subject><subject>Immediate-Early Proteins - immunology</subject><subject>Lymphocyte Activation</subject><subject>Molecular Sequence Data</subject><subject>Original Papers</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - chemistry</subject><subject>Peptides - immunology</subject><subject>Receptors, Interleukin-2</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - immunology</subject><issn>0882-8245</issn><issn>1557-8976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1r3DAQhkVJaTZpz70FkUMvQRvJsrTysbj5gkBDmp6FLI0TBdvaSvLC9tdXyy4p5KRheOZFMw9CXxldMqqay40flxWlYsnUkjfsA1owIVZENSt5hBZUqYqoqhbH6CSlV0qpkop_QsdMUi4awRZoczuPZsLtNocRns0QNj7Oifxag_W9t7j9UV_gJ9LCMOB2CBMk_Ag2PE_-L-A2hpTIIxib_QbwA6yzd4W4jmHE-QXw3TiC8yYDvjJx2GKGH2LI4KfP6GNvhgRfDu8p-n199dTekvufN3ft93tiuaSZdLQCtwLhOOOiYgKEUsw418m6M6W0tnF9rZQyjstaMlY6_apxruYCuFL8FH3b565j-DNDynr0yZZlzARhTloqKgWXVQHP34GvYY5T-ZuuWCNqSiUv0OUesrvFI_R6Hf1o4lYzqnc-dPGhdz40U7r4KBNnh9i5K6f4zx8EFIDsgV3bTNPgoYOY38D3gf8AjEiVyw</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Le Roy, Emmanuelle</creator><creator>Davignon, Jean-Luc</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein</title><author>Le Roy, Emmanuelle ; Davignon, Jean-Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-b02ed7e5d3135215e5881addb64ba881cc9df4888ad3646111ccf79dd435e3883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>Clone Cells - immunology</topic><topic>Cross Reactions</topic><topic>Cytomegalovirus - immunology</topic><topic>Cytomegalovirus - metabolism</topic><topic>HLA-DR Antigens - metabolism</topic><topic>HLA-DR Serological Subtypes</topic><topic>Humans</topic><topic>Immediate-Early Proteins - chemistry</topic><topic>Immediate-Early Proteins - immunology</topic><topic>Lymphocyte Activation</topic><topic>Molecular Sequence Data</topic><topic>Original Papers</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - chemistry</topic><topic>Peptides - immunology</topic><topic>Receptors, Interleukin-2</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Le Roy, Emmanuelle</creatorcontrib><creatorcontrib>Davignon, Jean-Luc</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Viral immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Le Roy, Emmanuelle</au><au>Davignon, Jean-Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein</atitle><jtitle>Viral immunology</jtitle><addtitle>Viral Immunol</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>18</volume><issue>2</issue><spage>391</spage><epage>396</epage><pages>391-396</pages><issn>0882-8245</issn><eissn>1557-8976</eissn><abstract>Human cytomegalovirus (HCMV) is a β-herpes virus that persists in a latent state in immunocompetent
individuals. Both CD4
+
and CD8
+
T lymphocytes have been reported to be present at a high
frequency in HCMV-seropositive individuals and are involved in the control of infection. How such
frequencies are maintained is not completely understood. We have observed that the canonical HLADR8
epitope of the immediate early 1 protein (IE1) contained in the IE1 (156-175) sequence shares
homologies with an IE1 sequence contained in part in the previously reported HLA-DR3 epitope,
IE1 (91-110). We thus wondered whether such homology in a single protein would translate into
recognition of the IE1 homolog sequence by HLA-DR8-restricted CD4
+
cells in addition to the
canonical epitope. We found that established HLA-DR8-restricted T cell clones are also able to
cross-recognize the IE1 (91-110) peptide, as well as a shorter 14-mer, IE1 (91-104). Moreover, the
homolog peptide IE1 (91-110) was able to generate, from a seropositive blood donor, new IE1-specific,
HLA-DR8-restricted CD4
+
T cell clones that were also cross-reactive. Those findings may
provide clues to the formation and regulation of the T-cell repertoire and memory.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>16035951</pmid><doi>10.1089/vim.2005.18.391</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-8245 |
| ispartof | Viral immunology, 2005-06, Vol.18 (2), p.391-396 |
| issn | 0882-8245 1557-8976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68065362 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Amino Acid Sequence CD4-Positive T-Lymphocytes - immunology Clone Cells - immunology Cross Reactions Cytomegalovirus - immunology Cytomegalovirus - metabolism HLA-DR Antigens - metabolism HLA-DR Serological Subtypes Humans Immediate-Early Proteins - chemistry Immediate-Early Proteins - immunology Lymphocyte Activation Molecular Sequence Data Original Papers Peptides - chemical synthesis Peptides - chemistry Peptides - immunology Receptors, Interleukin-2 Viral Proteins - chemistry Viral Proteins - immunology |
| title | Human Cytomegalovirus-Specific CD4+ T-Cell Clones Recognize Cross-Reactive Peptides From the Immediate Early 1 Protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T17%3A43%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20Cytomegalovirus-Specific%20CD4+%20T-Cell%20Clones%20Recognize%20Cross-Reactive%20Peptides%20From%20the%20Immediate%20Early%201%20Protein&rft.jtitle=Viral%20immunology&rft.au=Le%20Roy,%20Emmanuelle&rft.date=2005-06-01&rft.volume=18&rft.issue=2&rft.spage=391&rft.epage=396&rft.pages=391-396&rft.issn=0882-8245&rft.eissn=1557-8976&rft_id=info:doi/10.1089/vim.2005.18.391&rft_dat=%3Cproquest_cross%3E68065362%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=219540063&rft_id=info:pmid/16035951&rfr_iscdi=true |