Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout
The alpha subunit of the eighth complement component (C8) is a single-chain plasma glycoprotein which functions in the cytolytic process mediated by the complement system through a sequence of polymerization reactions with other terminal components. We have previously isolated and characterized the...
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| Veröffentlicht in: | Molecular Immunology 2006-07, Vol.43 (14), p.2188-2194 |
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| creator | Papanastasiou, Anastasios D. Zarkadis, Ioannis K. |
| description | The alpha subunit of the eighth complement component (C8) is a single-chain plasma glycoprotein which functions in the cytolytic process mediated by the complement system through a sequence of polymerization reactions with other terminal components. We have previously isolated and characterized the C8β and C8γ subunits of the eighth complement component in rainbow trout (
Oncorhynchus mykiss). Here, we report the primary sequence, the tissue expression profile, the domain architecture and the phylogenetic analysis of the trout C8α gene. The deduced amino acid sequence of the trout C8α gene exhibits 44 and 43% identity with human and frog orthologs, respectively. The domain architecture of the trout C8α resembles that of mammalian orthologs, and the cysteine backbone shows a high degree of conservation. The trout C8α shows a similar expression profile with that of trout C8β and C8γ, pointing to the liver as the main source of the C8 genes expression. Although the presence of a fully developed lytic pathway of complement system is expected in teleost, this is the first report of the C8α gene in an organism other than mammalian. |
| doi_str_mv | 10.1016/j.molimm.2006.01.004 |
| format | Article |
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Oncorhynchus mykiss). Here, we report the primary sequence, the tissue expression profile, the domain architecture and the phylogenetic analysis of the trout C8α gene. The deduced amino acid sequence of the trout C8α gene exhibits 44 and 43% identity with human and frog orthologs, respectively. The domain architecture of the trout C8α resembles that of mammalian orthologs, and the cysteine backbone shows a high degree of conservation. The trout C8α shows a similar expression profile with that of trout C8β and C8γ, pointing to the liver as the main source of the C8 genes expression. Although the presence of a fully developed lytic pathway of complement system is expected in teleost, this is the first report of the C8α gene in an organism other than mammalian.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.molimm.2006.01.004</identifier><identifier>PMID: 16483657</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Anura ; C8α ; Cloning, Molecular ; Comparative immunology ; Complement C8 - chemistry ; Complement C8 - genetics ; Complement lytic pathway ; Evolution ; Gene Dosage ; Molecular Sequence Data ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; Oncorhynchus mykiss - immunology ; Organ Specificity ; Phylogeny ; Protein Subunits - chemistry ; Protein Subunits - genetics ; Rainbow trout ; Sequence Alignment ; Teleostei</subject><ispartof>Molecular Immunology, 2006-07, Vol.43 (14), p.2188-2194</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-7a24289df85257970ebb8b33b71a4459ae3346a532e93e66511fc5f57a3ba6e83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.molimm.2006.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16483657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Papanastasiou, Anastasios D.</creatorcontrib><creatorcontrib>Zarkadis, Ioannis K.</creatorcontrib><title>Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout</title><title>Molecular Immunology</title><addtitle>Mol Immunol</addtitle><description>The alpha subunit of the eighth complement component (C8) is a single-chain plasma glycoprotein which functions in the cytolytic process mediated by the complement system through a sequence of polymerization reactions with other terminal components. We have previously isolated and characterized the C8β and C8γ subunits of the eighth complement component in rainbow trout (
Oncorhynchus mykiss). Here, we report the primary sequence, the tissue expression profile, the domain architecture and the phylogenetic analysis of the trout C8α gene. The deduced amino acid sequence of the trout C8α gene exhibits 44 and 43% identity with human and frog orthologs, respectively. The domain architecture of the trout C8α resembles that of mammalian orthologs, and the cysteine backbone shows a high degree of conservation. The trout C8α shows a similar expression profile with that of trout C8β and C8γ, pointing to the liver as the main source of the C8 genes expression. Although the presence of a fully developed lytic pathway of complement system is expected in teleost, this is the first report of the C8α gene in an organism other than mammalian.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Anura</subject><subject>C8α</subject><subject>Cloning, Molecular</subject><subject>Comparative immunology</subject><subject>Complement C8 - chemistry</subject><subject>Complement C8 - genetics</subject><subject>Complement lytic pathway</subject><subject>Evolution</subject><subject>Gene Dosage</subject><subject>Molecular Sequence Data</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Oncorhynchus mykiss - immunology</subject><subject>Organ Specificity</subject><subject>Phylogeny</subject><subject>Protein Subunits - chemistry</subject><subject>Protein Subunits - genetics</subject><subject>Rainbow trout</subject><subject>Sequence Alignment</subject><subject>Teleostei</subject><issn>0161-5890</issn><issn>1872-9142</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2r1DAQhoMonj1H_4FIrkQvWpPmq70RZPELDnij1yFtp9ssaVKT9Mj-e7Puind6NcPwzAy8D0IvKKkpofLtsV6Cs8tSN4TImtCaEP4I7WirmqqjvHmMdgWjlWg7coNuUzqSAhIpnqIbKnnLpFA7FPYueOsP2PgRr_PJhQN4yHYoA-NOySYcJpxnwMats8Fp6zdv858h2MOcZzyEZXWwgM-_2-DP3et9-wZbj6Oxvg8_cY5hy8_Qk8m4BM-v9Q59__jh2_5zdf_105f9-_tq4ELlSpmGN203Tq1ohOoUgb5ve8Z6RQ3nojPAGJdGsAY6BlIKSqdBTEIZ1hsJLbtDry531xh-bJCyXmwawDnjIWxJy7YkwTj9L0gV7Uh5UEB-AYcYUoow6TXaxcSTpkSfjeijvhjRZyOaUF2MlLWX1_tbv8D4d-mqoADvLgCUOB4sRJ0GC36A0UYYsh6D_feHX7jpntk</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Papanastasiou, Anastasios D.</creator><creator>Zarkadis, Ioannis K.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060701</creationdate><title>Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout</title><author>Papanastasiou, Anastasios D. ; Zarkadis, Ioannis K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-7a24289df85257970ebb8b33b71a4459ae3346a532e93e66511fc5f57a3ba6e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Anura</topic><topic>C8α</topic><topic>Cloning, Molecular</topic><topic>Comparative immunology</topic><topic>Complement C8 - chemistry</topic><topic>Complement C8 - genetics</topic><topic>Complement lytic pathway</topic><topic>Evolution</topic><topic>Gene Dosage</topic><topic>Molecular Sequence Data</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Oncorhynchus mykiss - immunology</topic><topic>Organ Specificity</topic><topic>Phylogeny</topic><topic>Protein Subunits - chemistry</topic><topic>Protein Subunits - genetics</topic><topic>Rainbow trout</topic><topic>Sequence Alignment</topic><topic>Teleostei</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papanastasiou, Anastasios D.</creatorcontrib><creatorcontrib>Zarkadis, Ioannis K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papanastasiou, Anastasios D.</au><au>Zarkadis, Ioannis K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout</atitle><jtitle>Molecular Immunology</jtitle><addtitle>Mol Immunol</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>43</volume><issue>14</issue><spage>2188</spage><epage>2194</epage><pages>2188-2194</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><eissn>1365-2567</eissn><abstract>The alpha subunit of the eighth complement component (C8) is a single-chain plasma glycoprotein which functions in the cytolytic process mediated by the complement system through a sequence of polymerization reactions with other terminal components. We have previously isolated and characterized the C8β and C8γ subunits of the eighth complement component in rainbow trout (
Oncorhynchus mykiss). Here, we report the primary sequence, the tissue expression profile, the domain architecture and the phylogenetic analysis of the trout C8α gene. The deduced amino acid sequence of the trout C8α gene exhibits 44 and 43% identity with human and frog orthologs, respectively. The domain architecture of the trout C8α resembles that of mammalian orthologs, and the cysteine backbone shows a high degree of conservation. The trout C8α shows a similar expression profile with that of trout C8β and C8γ, pointing to the liver as the main source of the C8 genes expression. Although the presence of a fully developed lytic pathway of complement system is expected in teleost, this is the first report of the C8α gene in an organism other than mammalian.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16483657</pmid><doi>10.1016/j.molimm.2006.01.004</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Amino Acid Sequence Animals Anura C8α Cloning, Molecular Comparative immunology Complement C8 - chemistry Complement C8 - genetics Complement lytic pathway Evolution Gene Dosage Molecular Sequence Data Oncorhynchus mykiss Oncorhynchus mykiss - genetics Oncorhynchus mykiss - immunology Organ Specificity Phylogeny Protein Subunits - chemistry Protein Subunits - genetics Rainbow trout Sequence Alignment Teleostei |
| title | Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout |
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