alpha-Tocopherol affects neuronal plasticity in adult rat dentate gyrus: the possible role of PKCdelta
Hippocampus dentate gyrus (DG) is characterized by neuronal plasticity processes in adulthood, and polysialylation of NCAM promotes neuronal plasticity. In previous investigations we found that alpha-tocopherol increased the PSA-NCAM-positive granule cell number in adult rat DG, suggesting that alph...
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| Veröffentlicht in: | Journal of neurobiology 2006-07, Vol.66 (8), p.793-810 |
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| creator | Ferri, Paola Cecchini, Tiziana Ambrogini, Patrizia Betti, Michele Cuppini, Riccardo Del Grande, Paolo Ciaroni, Sandra |
| description | Hippocampus dentate gyrus (DG) is characterized by neuronal plasticity processes in adulthood, and polysialylation of NCAM promotes neuronal plasticity. In previous investigations we found that alpha-tocopherol increased the PSA-NCAM-positive granule cell number in adult rat DG, suggesting that alpha-tocopherol may enhance neuronal plasticity. To verify this hypothesis, in the present study, structural remodeling in adult rat DG was investigated under alpha-tocopherol supplementation conditions. PSA-NCAM expression was evaluated by Western blotting, evaluation of PSA-NCAM-positive granule cell density, and morphometric analysis of PSA-NCAM-positive processes. In addition, the optical density of synaptophysin immunoreactivity and the synaptic profile density, examined by electron microscopy, were evaluated. Moreover, considering that PSA-NCAM expression has been found to be related to PKCdelta activity and alpha-tocopherol has been shown to inhibit PKC activity in vitro, Western blotting and immunohistochemistry followed by densitometry were used to analyze PKC. Our results demonstrated that an increase in PSA-NCAM expression and optical density of DG molecular layer synaptophysin immunoreactivity occurred in alpha-tocopherol-treated rats. Electron microscopy analysis showed that the increase in synaptophysin expression was related to an increase in synaptic profile density. In addition, Western blotting revealed a decrease in phospho-PKC Pan and phospho-PKCdelta, demonstrating that alpha-tocopherol is also able to inhibit PKC activity in vivo. Likewise, immunoreactivity for the active form of PKCdelta was lower in alpha-tocopherol-treated rats than in controls, while no changes were found in PKCdelta expression. These results demonstrate that alpha-tocopherol is an exogenous factor affecting neuronal plasticity in adult rat DG, possibly through PKCdelta inhibition. |
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In previous investigations we found that alpha-tocopherol increased the PSA-NCAM-positive granule cell number in adult rat DG, suggesting that alpha-tocopherol may enhance neuronal plasticity. To verify this hypothesis, in the present study, structural remodeling in adult rat DG was investigated under alpha-tocopherol supplementation conditions. PSA-NCAM expression was evaluated by Western blotting, evaluation of PSA-NCAM-positive granule cell density, and morphometric analysis of PSA-NCAM-positive processes. In addition, the optical density of synaptophysin immunoreactivity and the synaptic profile density, examined by electron microscopy, were evaluated. Moreover, considering that PSA-NCAM expression has been found to be related to PKCdelta activity and alpha-tocopherol has been shown to inhibit PKC activity in vitro, Western blotting and immunohistochemistry followed by densitometry were used to analyze PKC. Our results demonstrated that an increase in PSA-NCAM expression and optical density of DG molecular layer synaptophysin immunoreactivity occurred in alpha-tocopherol-treated rats. Electron microscopy analysis showed that the increase in synaptophysin expression was related to an increase in synaptic profile density. In addition, Western blotting revealed a decrease in phospho-PKC Pan and phospho-PKCdelta, demonstrating that alpha-tocopherol is also able to inhibit PKC activity in vivo. Likewise, immunoreactivity for the active form of PKCdelta was lower in alpha-tocopherol-treated rats than in controls, while no changes were found in PKCdelta expression. These results demonstrate that alpha-tocopherol is an exogenous factor affecting neuronal plasticity in adult rat DG, possibly through PKCdelta inhibition.</description><identifier>ISSN: 0022-3034</identifier><identifier>PMID: 16673395</identifier><language>eng</language><publisher>United States</publisher><subject>alpha-Tocopherol - pharmacology ; Animals ; Antioxidants - pharmacology ; Dendrites - metabolism ; Dendrites - ultrastructure ; Dentate Gyrus - drug effects ; Dentate Gyrus - enzymology ; Dentate Gyrus - ultrastructure ; Enzyme Inhibitors - pharmacology ; Immunohistochemistry ; Male ; Microscopy, Electron, Transmission ; Neural Cell Adhesion Molecule L1 - drug effects ; Neural Cell Adhesion Molecule L1 - metabolism ; Neuronal Plasticity - drug effects ; Neuronal Plasticity - physiology ; Presynaptic Terminals - drug effects ; Presynaptic Terminals - enzymology ; Presynaptic Terminals - ultrastructure ; Protein Kinase C-delta - antagonists & inhibitors ; Protein Kinase C-delta - metabolism ; Rats ; Rats, Sprague-Dawley ; Sialic Acids - metabolism ; Synaptic Membranes - drug effects ; Synaptic Membranes - enzymology ; Synaptic Membranes - ultrastructure ; Synaptophysin - drug effects ; Synaptophysin - metabolism ; Up-Regulation - drug effects ; Up-Regulation - physiology</subject><ispartof>Journal of neurobiology, 2006-07, Vol.66 (8), p.793-810</ispartof><rights>Copyright 2006 Wiley Periodicals, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16673395$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferri, Paola</creatorcontrib><creatorcontrib>Cecchini, Tiziana</creatorcontrib><creatorcontrib>Ambrogini, Patrizia</creatorcontrib><creatorcontrib>Betti, Michele</creatorcontrib><creatorcontrib>Cuppini, Riccardo</creatorcontrib><creatorcontrib>Del Grande, Paolo</creatorcontrib><creatorcontrib>Ciaroni, Sandra</creatorcontrib><title>alpha-Tocopherol affects neuronal plasticity in adult rat dentate gyrus: the possible role of PKCdelta</title><title>Journal of neurobiology</title><addtitle>J Neurobiol</addtitle><description>Hippocampus dentate gyrus (DG) is characterized by neuronal plasticity processes in adulthood, and polysialylation of NCAM promotes neuronal plasticity. In previous investigations we found that alpha-tocopherol increased the PSA-NCAM-positive granule cell number in adult rat DG, suggesting that alpha-tocopherol may enhance neuronal plasticity. To verify this hypothesis, in the present study, structural remodeling in adult rat DG was investigated under alpha-tocopherol supplementation conditions. PSA-NCAM expression was evaluated by Western blotting, evaluation of PSA-NCAM-positive granule cell density, and morphometric analysis of PSA-NCAM-positive processes. In addition, the optical density of synaptophysin immunoreactivity and the synaptic profile density, examined by electron microscopy, were evaluated. Moreover, considering that PSA-NCAM expression has been found to be related to PKCdelta activity and alpha-tocopherol has been shown to inhibit PKC activity in vitro, Western blotting and immunohistochemistry followed by densitometry were used to analyze PKC. Our results demonstrated that an increase in PSA-NCAM expression and optical density of DG molecular layer synaptophysin immunoreactivity occurred in alpha-tocopherol-treated rats. Electron microscopy analysis showed that the increase in synaptophysin expression was related to an increase in synaptic profile density. In addition, Western blotting revealed a decrease in phospho-PKC Pan and phospho-PKCdelta, demonstrating that alpha-tocopherol is also able to inhibit PKC activity in vivo. Likewise, immunoreactivity for the active form of PKCdelta was lower in alpha-tocopherol-treated rats than in controls, while no changes were found in PKCdelta expression. These results demonstrate that alpha-tocopherol is an exogenous factor affecting neuronal plasticity in adult rat DG, possibly through PKCdelta inhibition.</description><subject>alpha-Tocopherol - pharmacology</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Dendrites - metabolism</subject><subject>Dendrites - ultrastructure</subject><subject>Dentate Gyrus - drug effects</subject><subject>Dentate Gyrus - enzymology</subject><subject>Dentate Gyrus - ultrastructure</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Neural Cell Adhesion Molecule L1 - drug effects</subject><subject>Neural Cell Adhesion Molecule L1 - metabolism</subject><subject>Neuronal Plasticity - drug effects</subject><subject>Neuronal Plasticity - physiology</subject><subject>Presynaptic Terminals - drug effects</subject><subject>Presynaptic Terminals - enzymology</subject><subject>Presynaptic Terminals - ultrastructure</subject><subject>Protein Kinase C-delta - antagonists & inhibitors</subject><subject>Protein Kinase C-delta - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sialic Acids - metabolism</subject><subject>Synaptic Membranes - drug effects</subject><subject>Synaptic Membranes - enzymology</subject><subject>Synaptic Membranes - ultrastructure</subject><subject>Synaptophysin - drug effects</subject><subject>Synaptophysin - metabolism</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><issn>0022-3034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kL1OwzAURj2AaCm8AvLEFsmJ7SRmQxV_ohIM2aNr-5oGubGxnaFvTyXK9C1H50jfBVkz1jQVZ1ysyHXO34wxpWRzRVZ123acK7kmDnzcQzUEE-IeU_AUnENTMp1xSWEGT6OHXCYzlSOdZgp28YUmKNTiXKAg_TqmJT_QskcaQ86T9khPIqTB0c_3rUVf4IZcnkoZb8-7IcPz07B9rXYfL2_bx10VpZAVaiH6lgnbdsrUwsjOWW064ZSG2grNUAJzunFaoVQge6y1Be7AGOwt7_iG3P9pYwo_C-YyHqZs0HuYMSx5bHvWskb2J_DuDC76gHaMaTpAOo7_x_BfTl9gzg</recordid><startdate>200607</startdate><enddate>200607</enddate><creator>Ferri, Paola</creator><creator>Cecchini, Tiziana</creator><creator>Ambrogini, Patrizia</creator><creator>Betti, Michele</creator><creator>Cuppini, Riccardo</creator><creator>Del Grande, Paolo</creator><creator>Ciaroni, Sandra</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200607</creationdate><title>alpha-Tocopherol affects neuronal plasticity in adult rat dentate gyrus: the possible role of PKCdelta</title><author>Ferri, Paola ; Cecchini, Tiziana ; Ambrogini, Patrizia ; Betti, Michele ; Cuppini, Riccardo ; Del Grande, Paolo ; Ciaroni, Sandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p545-eb448604d679c14c57fdbc74f9ba1d4b0e5a0fb2fb9e59a58e1bda3facce8d373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>alpha-Tocopherol - pharmacology</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Dendrites - metabolism</topic><topic>Dendrites - ultrastructure</topic><topic>Dentate Gyrus - drug effects</topic><topic>Dentate Gyrus - enzymology</topic><topic>Dentate Gyrus - ultrastructure</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Neural Cell Adhesion Molecule L1 - drug effects</topic><topic>Neural Cell Adhesion Molecule L1 - metabolism</topic><topic>Neuronal Plasticity - drug effects</topic><topic>Neuronal Plasticity - physiology</topic><topic>Presynaptic Terminals - drug effects</topic><topic>Presynaptic Terminals - enzymology</topic><topic>Presynaptic Terminals - ultrastructure</topic><topic>Protein Kinase C-delta - antagonists & inhibitors</topic><topic>Protein Kinase C-delta - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sialic Acids - metabolism</topic><topic>Synaptic Membranes - drug effects</topic><topic>Synaptic Membranes - enzymology</topic><topic>Synaptic Membranes - ultrastructure</topic><topic>Synaptophysin - drug effects</topic><topic>Synaptophysin - metabolism</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Ferri, Paola</creatorcontrib><creatorcontrib>Cecchini, Tiziana</creatorcontrib><creatorcontrib>Ambrogini, Patrizia</creatorcontrib><creatorcontrib>Betti, Michele</creatorcontrib><creatorcontrib>Cuppini, Riccardo</creatorcontrib><creatorcontrib>Del Grande, Paolo</creatorcontrib><creatorcontrib>Ciaroni, Sandra</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferri, Paola</au><au>Cecchini, Tiziana</au><au>Ambrogini, Patrizia</au><au>Betti, Michele</au><au>Cuppini, Riccardo</au><au>Del Grande, Paolo</au><au>Ciaroni, Sandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>alpha-Tocopherol affects neuronal plasticity in adult rat dentate gyrus: the possible role of PKCdelta</atitle><jtitle>Journal of neurobiology</jtitle><addtitle>J Neurobiol</addtitle><date>2006-07</date><risdate>2006</risdate><volume>66</volume><issue>8</issue><spage>793</spage><epage>810</epage><pages>793-810</pages><issn>0022-3034</issn><abstract>Hippocampus dentate gyrus (DG) is characterized by neuronal plasticity processes in adulthood, and polysialylation of NCAM promotes neuronal plasticity. In previous investigations we found that alpha-tocopherol increased the PSA-NCAM-positive granule cell number in adult rat DG, suggesting that alpha-tocopherol may enhance neuronal plasticity. To verify this hypothesis, in the present study, structural remodeling in adult rat DG was investigated under alpha-tocopherol supplementation conditions. PSA-NCAM expression was evaluated by Western blotting, evaluation of PSA-NCAM-positive granule cell density, and morphometric analysis of PSA-NCAM-positive processes. In addition, the optical density of synaptophysin immunoreactivity and the synaptic profile density, examined by electron microscopy, were evaluated. Moreover, considering that PSA-NCAM expression has been found to be related to PKCdelta activity and alpha-tocopherol has been shown to inhibit PKC activity in vitro, Western blotting and immunohistochemistry followed by densitometry were used to analyze PKC. Our results demonstrated that an increase in PSA-NCAM expression and optical density of DG molecular layer synaptophysin immunoreactivity occurred in alpha-tocopherol-treated rats. Electron microscopy analysis showed that the increase in synaptophysin expression was related to an increase in synaptic profile density. In addition, Western blotting revealed a decrease in phospho-PKC Pan and phospho-PKCdelta, demonstrating that alpha-tocopherol is also able to inhibit PKC activity in vivo. Likewise, immunoreactivity for the active form of PKCdelta was lower in alpha-tocopherol-treated rats than in controls, while no changes were found in PKCdelta expression. These results demonstrate that alpha-tocopherol is an exogenous factor affecting neuronal plasticity in adult rat DG, possibly through PKCdelta inhibition.</abstract><cop>United States</cop><pmid>16673395</pmid><tpages>18</tpages></addata></record> |
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| subjects | alpha-Tocopherol - pharmacology Animals Antioxidants - pharmacology Dendrites - metabolism Dendrites - ultrastructure Dentate Gyrus - drug effects Dentate Gyrus - enzymology Dentate Gyrus - ultrastructure Enzyme Inhibitors - pharmacology Immunohistochemistry Male Microscopy, Electron, Transmission Neural Cell Adhesion Molecule L1 - drug effects Neural Cell Adhesion Molecule L1 - metabolism Neuronal Plasticity - drug effects Neuronal Plasticity - physiology Presynaptic Terminals - drug effects Presynaptic Terminals - enzymology Presynaptic Terminals - ultrastructure Protein Kinase C-delta - antagonists & inhibitors Protein Kinase C-delta - metabolism Rats Rats, Sprague-Dawley Sialic Acids - metabolism Synaptic Membranes - drug effects Synaptic Membranes - enzymology Synaptic Membranes - ultrastructure Synaptophysin - drug effects Synaptophysin - metabolism Up-Regulation - drug effects Up-Regulation - physiology |
| title | alpha-Tocopherol affects neuronal plasticity in adult rat dentate gyrus: the possible role of PKCdelta |
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