Genomic tools and cDNA derived markers for butterflies
The Lepidoptera have long been used as examples in the study of evolution, but some questions remain difficult to resolve due to a lack of molecular genetic data. However, as technology improves, genomic tools are becoming increasingly available to tackle unanswered evolutionary questions. Here we h...
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| Veröffentlicht in: | Molecular ecology 2005-08, Vol.14 (9), p.2883-2897 |
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| creator | Papanicolaou, A Joron, M McMillan, W.O Blaxter, M.L Jiggins, C.D |
| description | The Lepidoptera have long been used as examples in the study of evolution, but some questions remain difficult to resolve due to a lack of molecular genetic data. However, as technology improves, genomic tools are becoming increasingly available to tackle unanswered evolutionary questions. Here we have used expressed sequence tags (ESTs) to develop genetic markers for two Müllerian mimic species, Heliconius melpomene and Heliconius erato. In total 1363 ESTs were generated, representing 330 gene objects in H. melpomene and 431 in H. erato. User‐friendly bioinformatic tools were used to construct a nonredundant database of these putative genes (available at http://www.heliconius.org), and annotate them with blast similarity searches, InterPro matches and Gene Ontology terms. This database will be continually updated with EST sequences for the Papilionideae as they become publicly available, providing a tool for gene finding in the butterflies. Alignments of the Heliconius sequences with putative homologues derived from Bombyx mori or other public data sets were used to identify conserved PCR priming sites, and develop 55 markers that can be amplified from genomic DNA in both H. erato and H. melpomene. These markers will be used for comparative linkage mapping in Heliconius and will have applications in other phylogenetic and genomic studies in the Lepidoptera. |
| doi_str_mv | 10.1111/j.1365-294X.2005.02609.x |
| format | Article |
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However, as technology improves, genomic tools are becoming increasingly available to tackle unanswered evolutionary questions. Here we have used expressed sequence tags (ESTs) to develop genetic markers for two Müllerian mimic species, Heliconius melpomene and Heliconius erato. In total 1363 ESTs were generated, representing 330 gene objects in H. melpomene and 431 in H. erato. User‐friendly bioinformatic tools were used to construct a nonredundant database of these putative genes (available at http://www.heliconius.org), and annotate them with blast similarity searches, InterPro matches and Gene Ontology terms. This database will be continually updated with EST sequences for the Papilionideae as they become publicly available, providing a tool for gene finding in the butterflies. Alignments of the Heliconius sequences with putative homologues derived from Bombyx mori or other public data sets were used to identify conserved PCR priming sites, and develop 55 markers that can be amplified from genomic DNA in both H. erato and H. melpomene. 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However, as technology improves, genomic tools are becoming increasingly available to tackle unanswered evolutionary questions. Here we have used expressed sequence tags (ESTs) to develop genetic markers for two Müllerian mimic species, Heliconius melpomene and Heliconius erato. In total 1363 ESTs were generated, representing 330 gene objects in H. melpomene and 431 in H. erato. User‐friendly bioinformatic tools were used to construct a nonredundant database of these putative genes (available at http://www.heliconius.org), and annotate them with blast similarity searches, InterPro matches and Gene Ontology terms. This database will be continually updated with EST sequences for the Papilionideae as they become publicly available, providing a tool for gene finding in the butterflies. Alignments of the Heliconius sequences with putative homologues derived from Bombyx mori or other public data sets were used to identify conserved PCR priming sites, and develop 55 markers that can be amplified from genomic DNA in both H. erato and H. melpomene. These markers will be used for comparative linkage mapping in Heliconius and will have applications in other phylogenetic and genomic studies in the Lepidoptera.</description><subject>Adaptation, Biological - genetics</subject><subject>Animals</subject><subject>bioinformatics</subject><subject>Bombyx mori</subject><subject>Butterflies & moths</subject><subject>Butterflies - genetics</subject><subject>cDNA libraries</subject><subject>cDNA library</subject><subject>Chromosome Mapping</subject><subject>colour pattern evolution</subject><subject>complementary DNA</subject><subject>Computational Biology - methods</subject><subject>Databases, Genetic</subject><subject>DNA Primers</subject><subject>ecological genomics</subject><subject>Evolution, Molecular</subject><subject>Expressed Sequence Tags</subject><subject>Gene Library</subject><subject>Genetic linkage</subject><subject>Genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>Genomics</subject><subject>Genomics - methods</subject><subject>Heliconius</subject><subject>Heliconius erato</subject><subject>Heliconius melpomene</subject><subject>Lepidoptera</subject><subject>marker development</subject><subject>mimicry</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>Nymphalidae</subject><subject>Quantitative genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><issn>0962-1083</issn><issn>1365-294X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2P0zAQhi0EYruFvwARB24JY7ux4wOHVVkKol0kYAFxGeVjjFLSerGTpfvvcUi1SFwWX2zZzzuy5zFjCYeMx_Fim3Gp8lSYxddMAOQZCAUmO9xjs9uD-2wGRomUQyFP2GkIWwAuRZ4_ZCdcQUQKNWNqRXu3a-ukd64LSblvkvrVxVnSkG-vqUl2pf9BPiTW-aQa-p687VoKj9gDW3aBHh_nObt8ff5p-SZdv1-9XZ6t0zovwKRVzQ3pSpQ1KSOsjHdrGlEJrcg0JDQJIW2tLYgq5yCVrZQtBNRxQ5SVJTlnz6e6V979HCj0uGtDTV1X7skNAVUBCkCrO0GupQYdWzFnz_4Bt27w-_gIFByUMdzwO6EFNxChYoJq70LwZPHKt7FfN8gBR024xdEGjjZw1IR_NOEhRp8c6w_Vjpq_waOXCLycgF9tRzf_XRg358txFfPplG9DT4fbfJSJKnYixy8XKxQfPm_Wm3dL_Bb5pxNvS4fld98GvPwo4n8BDlzpRS5_A3vztv0</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Papanicolaou, A</creator><creator>Joron, M</creator><creator>McMillan, W.O</creator><creator>Blaxter, M.L</creator><creator>Jiggins, C.D</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>Genomic tools and cDNA derived markers for butterflies</title><author>Papanicolaou, A ; Joron, M ; McMillan, W.O ; Blaxter, M.L ; Jiggins, C.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5809-bc19e7b2ace692f3365dd2b276e9de27e223fc7f02b51036fb6f820cf022abfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adaptation, Biological - genetics</topic><topic>Animals</topic><topic>bioinformatics</topic><topic>Bombyx mori</topic><topic>Butterflies & moths</topic><topic>Butterflies - genetics</topic><topic>cDNA libraries</topic><topic>cDNA library</topic><topic>Chromosome Mapping</topic><topic>colour pattern evolution</topic><topic>complementary DNA</topic><topic>Computational Biology - methods</topic><topic>Databases, Genetic</topic><topic>DNA Primers</topic><topic>ecological genomics</topic><topic>Evolution, Molecular</topic><topic>Expressed Sequence Tags</topic><topic>Gene Library</topic><topic>Genetic linkage</topic><topic>Genetic markers</topic><topic>Genetic Markers - genetics</topic><topic>Genomics</topic><topic>Genomics - methods</topic><topic>Heliconius</topic><topic>Heliconius erato</topic><topic>Heliconius melpomene</topic><topic>Lepidoptera</topic><topic>marker development</topic><topic>mimicry</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Nymphalidae</topic><topic>Quantitative genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papanicolaou, A</creatorcontrib><creatorcontrib>Joron, M</creatorcontrib><creatorcontrib>McMillan, W.O</creatorcontrib><creatorcontrib>Blaxter, M.L</creatorcontrib><creatorcontrib>Jiggins, C.D</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papanicolaou, A</au><au>Joron, M</au><au>McMillan, W.O</au><au>Blaxter, M.L</au><au>Jiggins, C.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic tools and cDNA derived markers for butterflies</atitle><jtitle>Molecular ecology</jtitle><addtitle>Mol Ecol</addtitle><date>2005-08</date><risdate>2005</risdate><volume>14</volume><issue>9</issue><spage>2883</spage><epage>2897</epage><pages>2883-2897</pages><issn>0962-1083</issn><eissn>1365-294X</eissn><abstract>The Lepidoptera have long been used as examples in the study of evolution, but some questions remain difficult to resolve due to a lack of molecular genetic data. However, as technology improves, genomic tools are becoming increasingly available to tackle unanswered evolutionary questions. Here we have used expressed sequence tags (ESTs) to develop genetic markers for two Müllerian mimic species, Heliconius melpomene and Heliconius erato. In total 1363 ESTs were generated, representing 330 gene objects in H. melpomene and 431 in H. erato. User‐friendly bioinformatic tools were used to construct a nonredundant database of these putative genes (available at http://www.heliconius.org), and annotate them with blast similarity searches, InterPro matches and Gene Ontology terms. This database will be continually updated with EST sequences for the Papilionideae as they become publicly available, providing a tool for gene finding in the butterflies. Alignments of the Heliconius sequences with putative homologues derived from Bombyx mori or other public data sets were used to identify conserved PCR priming sites, and develop 55 markers that can be amplified from genomic DNA in both H. erato and H. melpomene. These markers will be used for comparative linkage mapping in Heliconius and will have applications in other phylogenetic and genomic studies in the Lepidoptera.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16029486</pmid><doi>10.1111/j.1365-294X.2005.02609.x</doi><tpages>15</tpages></addata></record> |
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| ispartof | Molecular ecology, 2005-08, Vol.14 (9), p.2883-2897 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adaptation, Biological - genetics Animals bioinformatics Bombyx mori Butterflies & moths Butterflies - genetics cDNA libraries cDNA library Chromosome Mapping colour pattern evolution complementary DNA Computational Biology - methods Databases, Genetic DNA Primers ecological genomics Evolution, Molecular Expressed Sequence Tags Gene Library Genetic linkage Genetic markers Genetic Markers - genetics Genomics Genomics - methods Heliconius Heliconius erato Heliconius melpomene Lepidoptera marker development mimicry molecular sequence data nucleotide sequences Nymphalidae Quantitative genetics Sequence Analysis, DNA Species Specificity |
| title | Genomic tools and cDNA derived markers for butterflies |
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