Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus
We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of t...
Gespeichert in:
| Veröffentlicht in: | Virus research 2005-09, Vol.112 (1), p.38-41 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 41 |
|---|---|
| container_issue | 1 |
| container_start_page | 38 |
| container_title | Virus research |
| container_volume | 112 |
| creator | Abe, Takumi Miyake, Norifumi Nishijima, Yasuyuki Fujita, Ryousuke Sahara, Ken Asano, Shin-ichiro Bando, Hisanori |
| description | We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant
Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in
Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase. |
| doi_str_mv | 10.1016/j.virusres.2005.03.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68053361</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168170205000857</els_id><sourcerecordid>17533050</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-38c2edb108abe8bb28089ec2993ebbe5a6332a991c08e92ade4a8b0a765c1a3d3</originalsourceid><addsrcrecordid>eNqFkUFr3DAQhUVoSLZp_0LQqTe7I2ktS7eEkLSFQA9NrhGSPGa12NZWshP676vNbulxQSAGfU9veI-QawY1Aya_buvXkJacMNccoKlB1MD0GVkx1fKqXWv-gawKqCrWAr8kH3PeAoAUrbwgl0wC50rrFXm5nzZ28jjiNNPYU2-XIfRDfMNEx5ht8PTdiIrmF92lOMa5vISpnIx-ph6HIZehLwN29C3MG-qsX4b4LvtEzns7ZPx8vK_I88P909336vHntx93t4-VF7qdK6E8x84xUNahco4rUBo911qgc9hYKQS3WjMPCjW3Ha6tcmBb2XhmRSeuyJfDv2XF3wvm2Ywh73ezE8YlG6mgEUKykyBrCwcNFFAeQJ9iLjn3ZpfCaNMfw8DsKzBb868Cs6_AgDClgiK8PjosbsTuv-yYeQFuDgCWQF4DJpN9wNJBF1JJ0XQxnPL4C4_rnVs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17533050</pqid></control><display><type>article</type><title>Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Abe, Takumi ; Miyake, Norifumi ; Nishijima, Yasuyuki ; Fujita, Ryousuke ; Sahara, Ken ; Asano, Shin-ichiro ; Bando, Hisanori</creator><creatorcontrib>Abe, Takumi ; Miyake, Norifumi ; Nishijima, Yasuyuki ; Fujita, Ryousuke ; Sahara, Ken ; Asano, Shin-ichiro ; Bando, Hisanori</creatorcontrib><description>We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant
Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in
Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2005.03.019</identifier><identifier>PMID: 16022899</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>35S promoter ; Animals ; Autographa californica ; Baculovirus ; Base Sequence ; CaMV ; Cauliflower mosaic virus ; Caulimovirus - genetics ; Cells, Cultured ; DNA-Directed RNA Polymerases - genetics ; DNA-Directed RNA Polymerases - metabolism ; Gene Expression Regulation, Viral ; Luciferases - genetics ; Luciferases - metabolism ; Molecular Sequence Data ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - genetics ; Nucleopolyhedrovirus - physiology ; Promoter Regions, Genetic - genetics ; Promoter Regions, Genetic - physiology ; Recombination, Genetic ; Spodoptera - virology ; Spodoptera frugiperda ; Transcription, Genetic ; Viral-induced RNA polymerase</subject><ispartof>Virus research, 2005-09, Vol.112 (1), p.38-41</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-38c2edb108abe8bb28089ec2993ebbe5a6332a991c08e92ade4a8b0a765c1a3d3</citedby><cites>FETCH-LOGICAL-c397t-38c2edb108abe8bb28089ec2993ebbe5a6332a991c08e92ade4a8b0a765c1a3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2005.03.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16022899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, Takumi</creatorcontrib><creatorcontrib>Miyake, Norifumi</creatorcontrib><creatorcontrib>Nishijima, Yasuyuki</creatorcontrib><creatorcontrib>Fujita, Ryousuke</creatorcontrib><creatorcontrib>Sahara, Ken</creatorcontrib><creatorcontrib>Asano, Shin-ichiro</creatorcontrib><creatorcontrib>Bando, Hisanori</creatorcontrib><title>Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant
Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in
Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.</description><subject>35S promoter</subject><subject>Animals</subject><subject>Autographa californica</subject><subject>Baculovirus</subject><subject>Base Sequence</subject><subject>CaMV</subject><subject>Cauliflower mosaic virus</subject><subject>Caulimovirus - genetics</subject><subject>Cells, Cultured</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Gene Expression Regulation, Viral</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Nuclear polyhedrosis virus</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>Nucleopolyhedrovirus - physiology</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Recombination, Genetic</subject><subject>Spodoptera - virology</subject><subject>Spodoptera frugiperda</subject><subject>Transcription, Genetic</subject><subject>Viral-induced RNA polymerase</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVoSLZp_0LQqTe7I2ktS7eEkLSFQA9NrhGSPGa12NZWshP676vNbulxQSAGfU9veI-QawY1Aya_buvXkJacMNccoKlB1MD0GVkx1fKqXWv-gawKqCrWAr8kH3PeAoAUrbwgl0wC50rrFXm5nzZ28jjiNNPYU2-XIfRDfMNEx5ht8PTdiIrmF92lOMa5vISpnIx-ph6HIZehLwN29C3MG-qsX4b4LvtEzns7ZPx8vK_I88P909336vHntx93t4-VF7qdK6E8x84xUNahco4rUBo911qgc9hYKQS3WjMPCjW3Ha6tcmBb2XhmRSeuyJfDv2XF3wvm2Ywh73ezE8YlG6mgEUKykyBrCwcNFFAeQJ9iLjn3ZpfCaNMfw8DsKzBb868Cs6_AgDClgiK8PjosbsTuv-yYeQFuDgCWQF4DJpN9wNJBF1JJ0XQxnPL4C4_rnVs</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Abe, Takumi</creator><creator>Miyake, Norifumi</creator><creator>Nishijima, Yasuyuki</creator><creator>Fujita, Ryousuke</creator><creator>Sahara, Ken</creator><creator>Asano, Shin-ichiro</creator><creator>Bando, Hisanori</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050901</creationdate><title>Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus</title><author>Abe, Takumi ; Miyake, Norifumi ; Nishijima, Yasuyuki ; Fujita, Ryousuke ; Sahara, Ken ; Asano, Shin-ichiro ; Bando, Hisanori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-38c2edb108abe8bb28089ec2993ebbe5a6332a991c08e92ade4a8b0a765c1a3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>35S promoter</topic><topic>Animals</topic><topic>Autographa californica</topic><topic>Baculovirus</topic><topic>Base Sequence</topic><topic>CaMV</topic><topic>Cauliflower mosaic virus</topic><topic>Caulimovirus - genetics</topic><topic>Cells, Cultured</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Gene Expression Regulation, Viral</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Nuclear polyhedrosis virus</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>Nucleopolyhedrovirus - physiology</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Recombination, Genetic</topic><topic>Spodoptera - virology</topic><topic>Spodoptera frugiperda</topic><topic>Transcription, Genetic</topic><topic>Viral-induced RNA polymerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, Takumi</creatorcontrib><creatorcontrib>Miyake, Norifumi</creatorcontrib><creatorcontrib>Nishijima, Yasuyuki</creatorcontrib><creatorcontrib>Fujita, Ryousuke</creatorcontrib><creatorcontrib>Sahara, Ken</creatorcontrib><creatorcontrib>Asano, Shin-ichiro</creatorcontrib><creatorcontrib>Bando, Hisanori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, Takumi</au><au>Miyake, Norifumi</au><au>Nishijima, Yasuyuki</au><au>Fujita, Ryousuke</au><au>Sahara, Ken</au><au>Asano, Shin-ichiro</au><au>Bando, Hisanori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>112</volume><issue>1</issue><spage>38</spage><epage>41</epage><pages>38-41</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant
Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in
Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16022899</pmid><doi>10.1016/j.virusres.2005.03.019</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1702 |
| ispartof | Virus research, 2005-09, Vol.112 (1), p.38-41 |
| issn | 0168-1702 1872-7492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68053361 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 35S promoter Animals Autographa californica Baculovirus Base Sequence CaMV Cauliflower mosaic virus Caulimovirus - genetics Cells, Cultured DNA-Directed RNA Polymerases - genetics DNA-Directed RNA Polymerases - metabolism Gene Expression Regulation, Viral Luciferases - genetics Luciferases - metabolism Molecular Sequence Data Nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Nucleopolyhedrovirus - physiology Promoter Regions, Genetic - genetics Promoter Regions, Genetic - physiology Recombination, Genetic Spodoptera - virology Spodoptera frugiperda Transcription, Genetic Viral-induced RNA polymerase |
| title | Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T17%3A19%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhancement%20of%20cauliflower%20mosaic%20virus%2035S%20promoter%20in%20insect%20cells%20infected%20with%20baculovirus&rft.jtitle=Virus%20research&rft.au=Abe,%20Takumi&rft.date=2005-09-01&rft.volume=112&rft.issue=1&rft.spage=38&rft.epage=41&rft.pages=38-41&rft.issn=0168-1702&rft.eissn=1872-7492&rft_id=info:doi/10.1016/j.virusres.2005.03.019&rft_dat=%3Cproquest_cross%3E17533050%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17533050&rft_id=info:pmid/16022899&rft_els_id=S0168170205000857&rfr_iscdi=true |