The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles
To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles. A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2006-03, Vol.23 (3), p.129-136 |
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| container_end_page | 136 |
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| container_issue | 3 |
| container_start_page | 129 |
| container_title | Journal of assisted reproduction and genetics |
| container_volume | 23 |
| creator | Wilding, Martin Singer, Michael Fehr, Peter Haeberlin, Felix Roth, Felix Lachat, Remo Di Matteo, Loredana Capobianco, Clemente Dale, Brian |
| description | To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles.
A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.
Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.
Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures. |
| doi_str_mv | 10.1007/s10815-005-9016-y |
| format | Article |
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A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.
Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.
Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.</description><identifier>ISSN: 1058-0468</identifier><identifier>DOI: 10.1007/s10815-005-9016-y</identifier><identifier>PMID: 16622803</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Adult ; Female ; Fertilization in Vitro - methods ; Humans ; Hydrogen-Ion Concentration ; Intracellular Membranes - metabolism ; Membrane Potentials ; Mitochondria - metabolism ; Oocytes - cytology ; Oocytes - metabolism ; Ovarian Follicle - cytology ; Ovary - cytology ; Pregnancy ; Pregnancy Rate ; Prospective Studies ; Specimen Handling ; Time Factors ; Treatment Outcome</subject><ispartof>Journal of assisted reproduction and genetics, 2006-03, Vol.23 (3), p.129-136</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16622803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilding, Martin</creatorcontrib><creatorcontrib>Singer, Michael</creatorcontrib><creatorcontrib>Fehr, Peter</creatorcontrib><creatorcontrib>Haeberlin, Felix</creatorcontrib><creatorcontrib>Roth, Felix</creatorcontrib><creatorcontrib>Lachat, Remo</creatorcontrib><creatorcontrib>Di Matteo, Loredana</creatorcontrib><creatorcontrib>Capobianco, Clemente</creatorcontrib><creatorcontrib>Dale, Brian</creatorcontrib><title>The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><description>To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles.
A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.
Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.
Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.</description><subject>Adult</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular Membranes - metabolism</subject><subject>Membrane Potentials</subject><subject>Mitochondria - metabolism</subject><subject>Oocytes - cytology</subject><subject>Oocytes - metabolism</subject><subject>Ovarian Follicle - cytology</subject><subject>Ovary - cytology</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Prospective Studies</subject><subject>Specimen Handling</subject><subject>Time Factors</subject><subject>Treatment Outcome</subject><issn>1058-0468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kL1OwzAYRT2AaCk8AAvyxBb4_JPEHlHFn1SJpcxRan8BIycOsY2at4eKMl3p3KM7XEKuGNwygPouMlCsLADKQgOrivmELBmUqgBZqQU5j_ETALTi4owsWFVxrkAsSdx-IMWuQ5No6CjuEw4WLTXZpzzhgZncZ59jEYKZE1IT-tHjHiN1A-2C9-7XbSfa-ewstXlyw_uh-nZpCrTDKTnvYptcGKiZjcd4QU671ke8POaKvD0-bNfPxeb16WV9vylGJnQquJAgtOa2qpmqjTW11qXUUjArQLVKm04gCi6lqUxbylpybStua834rgYuVuTmb3ecwlfGmJreRYPetwOGHJtKgVSgDuL1Ucy7Hm0zTq5vp7n5v0n8AEKcaW4</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Wilding, Martin</creator><creator>Singer, Michael</creator><creator>Fehr, Peter</creator><creator>Haeberlin, Felix</creator><creator>Roth, Felix</creator><creator>Lachat, Remo</creator><creator>Di Matteo, Loredana</creator><creator>Capobianco, Clemente</creator><creator>Dale, Brian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200603</creationdate><title>The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles</title><author>Wilding, Martin ; Singer, Michael ; Fehr, Peter ; Haeberlin, Felix ; Roth, Felix ; Lachat, Remo ; Di Matteo, Loredana ; Capobianco, Clemente ; Dale, Brian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p139t-23403992d67187cdc799549431d308a89cf3ee3244c6ca547429d62d7912b7023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular Membranes - metabolism</topic><topic>Membrane Potentials</topic><topic>Mitochondria - metabolism</topic><topic>Oocytes - cytology</topic><topic>Oocytes - metabolism</topic><topic>Ovarian Follicle - cytology</topic><topic>Ovary - cytology</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Prospective Studies</topic><topic>Specimen Handling</topic><topic>Time Factors</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilding, Martin</creatorcontrib><creatorcontrib>Singer, Michael</creatorcontrib><creatorcontrib>Fehr, Peter</creatorcontrib><creatorcontrib>Haeberlin, Felix</creatorcontrib><creatorcontrib>Roth, Felix</creatorcontrib><creatorcontrib>Lachat, Remo</creatorcontrib><creatorcontrib>Di Matteo, Loredana</creatorcontrib><creatorcontrib>Capobianco, Clemente</creatorcontrib><creatorcontrib>Dale, Brian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilding, Martin</au><au>Singer, Michael</au><au>Fehr, Peter</au><au>Haeberlin, Felix</au><au>Roth, Felix</au><au>Lachat, Remo</au><au>Di Matteo, Loredana</au><au>Capobianco, Clemente</au><au>Dale, Brian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><addtitle>J Assist Reprod Genet</addtitle><date>2006-03</date><risdate>2006</risdate><volume>23</volume><issue>3</issue><spage>129</spage><epage>136</epage><pages>129-136</pages><issn>1058-0468</issn><abstract>To assess the clinical and biological effect of the preincubation of oocytes in follicular fluid prior to IVF and ICSI cycles.
A series of patients were treated by the preincubation of oocytes in the patients' follicular fluid for 3 h after oocyte retrieval followed by processing with standard protocols. Control oocytes were preincubated in normal IVF culture medium. Fluorescence techniques were used to examine oocyte mitochondrial membrane potential.
Fertilisation, pregnancy, and implantation rates were all significantly improved after the preincubation of oocytes in follicular fluid. Further tests suggested that differences in pH between follicular fluid and artificial culture medium may be critical to these differences.
Preincubation of human oocytes in follicular fluid improves the results after IVF. This may be partly due to the use of a non-"physiological" pH in artificial culture media during in vitro fertilisation procedures.</abstract><cop>Netherlands</cop><pmid>16622803</pmid><doi>10.1007/s10815-005-9016-y</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of assisted reproduction and genetics, 2006-03, Vol.23 (3), p.129-136 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | Adult Female Fertilization in Vitro - methods Humans Hydrogen-Ion Concentration Intracellular Membranes - metabolism Membrane Potentials Mitochondria - metabolism Oocytes - cytology Oocytes - metabolism Ovarian Follicle - cytology Ovary - cytology Pregnancy Pregnancy Rate Prospective Studies Specimen Handling Time Factors Treatment Outcome |
| title | The effect of extended culture of cumulus-oocyte complexes in follicular fluid during in vitro fertilisation cycles |
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