Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1
Equine cytotoxic T lymphocyte (CTL) responses to equine herpesvirus-1 (EHV-1) are well characterised but little is known about the cytokine response after infection or vaccination. EHV-1 is common in horses and infects lymphocytes in vivo. This virus was used as a model to measure the synthesis of i...
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| Veröffentlicht in: | Vaccine 2005-08, Vol.23 (36), p.4541-4551 |
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| creator | Paillot, R. Daly, J.M. Juillard, V. Minke, J.M. Hannant, D. Kydd, J.H. |
| description | Equine cytotoxic T lymphocyte (CTL) responses to equine herpesvirus-1 (EHV-1) are well characterised but little is known about the cytokine response after infection or vaccination. EHV-1 is common in horses and infects lymphocytes in vivo. This virus was used as a model to measure the synthesis of interferon gamma (IFN-γ) by equine peripheral blood mononuclear cells (PBMC) after in vivo infection and/or in vitro stimulation with EHV-1. Both flow cytometry and ELISPOT assays were used to quantify equine IFN-γ using a mouse anti-bovine IFN-γ monoclonal antibody (clone CC302; shown to cross-react with recombinant equine IFN-γ) and a rabbit anti-canine IFN-γ polyclonal antibody. The percentage of PBMC synthesising IFN-γ after in vitro stimulation with EHV-1 increased with age. In yearlings infected experimentally with EHV-1, PBMC showed two peaks of IFN-γ synthesis, 11 and 56 days after infection. The IFN-γ synthesis was principally associated with CD8
+ cells. The patterns of IFN-γ synthesis detected by intracellular IFN-γ staining or ELISPOT were compared with CTL data and shown to be similar. These methods were also applied successfully to frozen samples of PBMC. Measurement of equine IFN-γ using these simple techniques can now be applied to future studies on protective cellular immune responses following virus infection and/or vaccination of horses. |
| doi_str_mv | 10.1016/j.vaccine.2005.03.048 |
| format | Article |
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+ cells. The patterns of IFN-γ synthesis detected by intracellular IFN-γ staining or ELISPOT were compared with CTL data and shown to be similar. These methods were also applied successfully to frozen samples of PBMC. Measurement of equine IFN-γ using these simple techniques can now be applied to future studies on protective cellular immune responses following virus infection and/or vaccination of horses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2005.03.048</identifier><identifier>PMID: 15913852</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Age Factors ; Animals ; Applied microbiology ; Biological and medical sciences ; Cryopreservation ; Cytokines ; ELISPOT assay ; Equine herpesvirus ; Equine herpesvirus-1 ; Equine IFN gamma ; Fundamental and applied biological sciences. Psychology ; Herpesviridae Infections - immunology ; Herpesviridae Infections - prevention & control ; Herpesviridae Infections - veterinary ; Herpesvirus 1, Equid - immunology ; Horse Diseases - immunology ; Horse Diseases - prevention & control ; Horses ; Immune system ; Interferon-gamma - biosynthesis ; Leukocytes, Mononuclear - immunology ; Lymphocytes ; Lymphocytes - immunology ; Male ; Microbiology ; Miscellaneous ; T-Lymphocytes, Cytotoxic - immunology ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Virology</subject><ispartof>Vaccine, 2005-08, Vol.23 (36), p.4541-4551</ispartof><rights>2005 Elsevier Ltd</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Elsevier Limited Aug 22, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-839c18f5525e1bcc3f3c3b79446eaa299b2c2b1e4b49e77f83b205284278d403</citedby><cites>FETCH-LOGICAL-c452t-839c18f5525e1bcc3f3c3b79446eaa299b2c2b1e4b49e77f83b205284278d403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1558978451?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976,64364,64366,64368,72218</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16955461$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15913852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paillot, R.</creatorcontrib><creatorcontrib>Daly, J.M.</creatorcontrib><creatorcontrib>Juillard, V.</creatorcontrib><creatorcontrib>Minke, J.M.</creatorcontrib><creatorcontrib>Hannant, D.</creatorcontrib><creatorcontrib>Kydd, J.H.</creatorcontrib><title>Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Equine cytotoxic T lymphocyte (CTL) responses to equine herpesvirus-1 (EHV-1) are well characterised but little is known about the cytokine response after infection or vaccination. EHV-1 is common in horses and infects lymphocytes in vivo. This virus was used as a model to measure the synthesis of interferon gamma (IFN-γ) by equine peripheral blood mononuclear cells (PBMC) after in vivo infection and/or in vitro stimulation with EHV-1. Both flow cytometry and ELISPOT assays were used to quantify equine IFN-γ using a mouse anti-bovine IFN-γ monoclonal antibody (clone CC302; shown to cross-react with recombinant equine IFN-γ) and a rabbit anti-canine IFN-γ polyclonal antibody. The percentage of PBMC synthesising IFN-γ after in vitro stimulation with EHV-1 increased with age. In yearlings infected experimentally with EHV-1, PBMC showed two peaks of IFN-γ synthesis, 11 and 56 days after infection. The IFN-γ synthesis was principally associated with CD8
+ cells. The patterns of IFN-γ synthesis detected by intracellular IFN-γ staining or ELISPOT were compared with CTL data and shown to be similar. These methods were also applied successfully to frozen samples of PBMC. Measurement of equine IFN-γ using these simple techniques can now be applied to future studies on protective cellular immune responses following virus infection and/or vaccination of horses.</description><subject>Age Factors</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Cryopreservation</subject><subject>Cytokines</subject><subject>ELISPOT assay</subject><subject>Equine herpesvirus</subject><subject>Equine herpesvirus-1</subject><subject>Equine IFN gamma</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesviridae Infections - immunology</subject><subject>Herpesviridae Infections - prevention & control</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesvirus 1, Equid - immunology</subject><subject>Horse Diseases - immunology</subject><subject>Horse Diseases - prevention & control</subject><subject>Horses</subject><subject>Immune system</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1r3DAQhkVpaLZpf0KLoaQ3u_q05FMpYdsUArmE0puQ5VFXiy1vJHvL_vvIXUOgl5wGZp53GOlB6APBFcGk_rKvjsZaH6CiGIsKswpz9QptiJKspIKo12iDac1LTvDvS_Q2pT3OICPNG3RJREOYEnSD-u3jnJcUPkwQHcQxFH_MMJgincK0g-RTHhX9aTjsRnuaIBXGZXJpHv1xzNWBnXyOmdCdu1McizT5Ye7Nv8FfP-2K7e2vkrxDF870Cd6v9Qo9fN8-3NyWd_c_ft58uystF3QqFWssUU4IKoC01jLHLGtlw3kNxtCmaamlLQHe8gakdIq1FAuqOJWq45hdoc_ntYc4Ps6QJj34ZKHvTYBxTrpWmEtM6YsgkVwKTBbw03_gfpxjyG_QRAjVSMUFyZQ4UzaOKUVw-hD9YOJJE6wXaXqvV2l6kaYx01lazn1ct8_tAN1zarWUgesVMMma3kUTrE_PXN0IwevlgK9nDvLnHj1EnayHYKHzMVvS3ehfOOUJF9i3hw</recordid><startdate>20050822</startdate><enddate>20050822</enddate><creator>Paillot, R.</creator><creator>Daly, J.M.</creator><creator>Juillard, V.</creator><creator>Minke, J.M.</creator><creator>Hannant, D.</creator><creator>Kydd, J.H.</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20050822</creationdate><title>Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1</title><author>Paillot, R. ; Daly, J.M. ; Juillard, V. ; Minke, J.M. ; Hannant, D. ; Kydd, J.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-839c18f5525e1bcc3f3c3b79446eaa299b2c2b1e4b49e77f83b205284278d403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Age Factors</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Cryopreservation</topic><topic>Cytokines</topic><topic>ELISPOT assay</topic><topic>Equine herpesvirus</topic><topic>Equine herpesvirus-1</topic><topic>Equine IFN gamma</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paillot, R.</au><au>Daly, J.M.</au><au>Juillard, V.</au><au>Minke, J.M.</au><au>Hannant, D.</au><au>Kydd, J.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2005-08-22</date><risdate>2005</risdate><volume>23</volume><issue>36</issue><spage>4541</spage><epage>4551</epage><pages>4541-4551</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Equine cytotoxic T lymphocyte (CTL) responses to equine herpesvirus-1 (EHV-1) are well characterised but little is known about the cytokine response after infection or vaccination. EHV-1 is common in horses and infects lymphocytes in vivo. This virus was used as a model to measure the synthesis of interferon gamma (IFN-γ) by equine peripheral blood mononuclear cells (PBMC) after in vivo infection and/or in vitro stimulation with EHV-1. Both flow cytometry and ELISPOT assays were used to quantify equine IFN-γ using a mouse anti-bovine IFN-γ monoclonal antibody (clone CC302; shown to cross-react with recombinant equine IFN-γ) and a rabbit anti-canine IFN-γ polyclonal antibody. The percentage of PBMC synthesising IFN-γ after in vitro stimulation with EHV-1 increased with age. In yearlings infected experimentally with EHV-1, PBMC showed two peaks of IFN-γ synthesis, 11 and 56 days after infection. The IFN-γ synthesis was principally associated with CD8
+ cells. The patterns of IFN-γ synthesis detected by intracellular IFN-γ staining or ELISPOT were compared with CTL data and shown to be similar. These methods were also applied successfully to frozen samples of PBMC. Measurement of equine IFN-γ using these simple techniques can now be applied to future studies on protective cellular immune responses following virus infection and/or vaccination of horses.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15913852</pmid><doi>10.1016/j.vaccine.2005.03.048</doi><tpages>11</tpages></addata></record> |
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| subjects | Age Factors Animals Applied microbiology Biological and medical sciences Cryopreservation Cytokines ELISPOT assay Equine herpesvirus Equine herpesvirus-1 Equine IFN gamma Fundamental and applied biological sciences. Psychology Herpesviridae Infections - immunology Herpesviridae Infections - prevention & control Herpesviridae Infections - veterinary Herpesvirus 1, Equid - immunology Horse Diseases - immunology Horse Diseases - prevention & control Horses Immune system Interferon-gamma - biosynthesis Leukocytes, Mononuclear - immunology Lymphocytes Lymphocytes - immunology Male Microbiology Miscellaneous T-Lymphocytes, Cytotoxic - immunology Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology |
| title | Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1 |
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